Results 71 to 80 of about 378,917 (194)

Distinct forebrain and cerebellar isozymes of type II Ca^(2+)/calmodulin-dependent protein kinase associate differently with the postsynaptic density fraction [PDF]

open access: yes, 1985
Forebrain and cerebellar Type II Ca2+/calmodulin-dependent protein kinases have different subunit compositions. The forebrain holoenzyme, characterized in our laboratory, is a 650-kDa holoenzyme composed of 50-kDa alpha-subunits and 60-kDa beta-subunits ...
Kennedy, Mary B., Miller, Stephen G.
core  

From colloidal dispersions to colloidal pastesthrough solid–liquid separation processes [PDF]

open access: yes, 2005
Solid–liquid separation is an operation that starts with a dispersion of solid particles in a liquid and removes some of the liquid from the particles, producing a concentrated solid paste and a clean liquid phase.
Botet, Robert   +6 more
core   +6 more sources

A laboratory modification to testicular sperm preparation technique improves spermatogenic cell yield

open access: yesAsian Journal of Andrology, 2014
Testicular sperm extraction is a common procedure used to find spermatogenic cells in men with nonobstructive azoospermia. The laboratory processing of biopsied testicular tissues needs to be performed meticulously to acquire a high yield of cells.
Sinan Ozkavukcu   +4 more
doaj   +1 more source

Density Gradient Centrifugation Studies of Rubella Virus

open access: yesExperimental Biology and Medicine, 1966
SummaryRubella virus was grown in three different mammalian kidney cell lines. The resulting virus-containing supernates were concentrated by forced dialysis and were then studied for density on sucrose gradients. All of the virus preparations produced peaked infectivity bands with 99% of the infec-tivity concentrated in a band from 1.040 to 1.120 g ...
openaire   +2 more sources

Deletion mutants of bacteriophage phiX174 [PDF]

open access: yes, 1972
Mutants of bacteriophage phiX174 have been isolated that are less dense than wild-type phiX particles in CsCl. When mutant viral (+) strand DNA and wild-type complementary (-) strand DNA are hybridized, the resulting duplex molecules have single-stranded
Benbow, Robert M.   +2 more
core  

Surface modification of Beckman Ultra-Clear centrifuge tubes for density gradient centrifugation of lipoproteins

open access: yesJournal of Lipid Research, 1982
A simple procedure for coating the interior surface of Beckman Ultra-Clear centrifuge tubes with polyvinyl alcohol is described. The coated tubes are wettable and allow salt solutions to gravity-feed down their sides.
L Holmquist
doaj   +1 more source

Biosynthetic pathway of mitochondrial ATPase subunit 9 in Neurospora crassa [PDF]

open access: yes, 1983
Subunit 9 of mitochondrial ATPase (Su9) is synthesized in reticulocyte lysates programmed with Neurospora poly A-RNA, and in a Neurospora cell free system as a precursor with a higher apparent molecular weight than the mature protein (Mr 16,400 vs.
Hennig, Bernd   +3 more
core   +1 more source

Abridged Ribosome Profiling for Accurate Bacterial Translation Measurements

open access: yesMethods and Protocols
Ribosome profiling, or Ribo-Seq, is a powerful tool for studying translation. It maps the positions of translating ribosomes on mRNAs, providing insights into actively expressed genes. Unlike mass spectrometry, Ribo-Seq is not affected by the same biases
Marc Follmer   +2 more
doaj   +1 more source

CENTRIFUGAÇÃO DE ESPERMATOZOIDES OVINOS EM GRADIENTE DE DENSIDADE CONTÍNUO: EFEITO SOBRE A TAXA DE PRENHEZ E PROPORÇÃO DO SEXO

open access: yesCiência Animal Brasileira, 2015
The objective of this study was to evaluate the efficiency of continuous density gradients of Percoll and OptiPrep for separation of X-bearing sperm, and assess the influence on sperm viability, pregnancy rate and sex ratio.
Max Vitória Resende   +5 more
doaj   +1 more source

Nature of {varphi}X174 Linear DNA from a DNA Ligase-Defective Host [PDF]

open access: yes, 1974
Linear DNAs have been prepared from {varphi}X phage and from {varphi}X RF II (double-stranded circular form of {varphi}X DNA, formed during infection and nicked in one or both strands) molecules derived from infection at the restrictive temperature of ...
Miller, Lois K., Sinsheimer, Robert L.
core  

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