Results 1 to 10 of about 240,760 (305)

Single-cell mass-density measurements using microchannel gradient centrifugation [PDF]

Scientific Reports
We present a microchannel-based adaptation of mass density-gradient centrifugation for the mass density measurement of cells or microparticles. The workflow consists of three basic steps: Microchannel filling, centrifugation, and microscopy. Microchannel
Richard Soller, Per Augustsson, Rune Barnkob   +2 more
doaj   +3 more sources

Mass Cytometry Reveals Comparable Proportions of Leukocyte Subsets and Cell Surface P2X7 or CD39 on Human Peripheral Blood Mononuclear Cell Samples Isolated by Ficoll-Paque or SepMate Tube Density Gradient Centrifugation [PDF]

Cells
P2X7 is an adenosine 5′-triphosphate (ATP)-gated ion channel. CD39 (ectonucleotidase triphosphate diphosphohydrolase-1) hydrolyses ATP to reduce its extracellular concentration to limit P2X7 activation.
Amal Elhage, Bavani Gunasegaran, Natalie Smith, Ross J. Turner, Debbie Watson, Helen M. McGuire, Ronald Sluyter   +6 more
doaj   +2 more sources

Improved progressive motility recovery with a standardized gradient-based approach for semen preparation in IUI [PDF]

Reproductive Biology and Endocrinology
Objective To evaluate the laboratory performance of a standardized gradient-based sperm preparation system designed to improve progressive motility recovery during density gradient centrifugation for intrauterine insemination (IUI). Design Retrospective,
Felix Roth, Felix Haeberlin
doaj   +2 more sources

Abridged Ribosome Profiling for Accurate Bacterial Translation Measurements [PDF]

Methods and Protocols
Ribosome profiling, or Ribo-Seq, is a powerful tool for studying translation. It maps the positions of translating ribosomes on mRNAs, providing insights into actively expressed genes. Unlike mass spectrometry, Ribo-Seq is not affected by the same biases
Marc Follmer, Korbinian Pürckhauer, Klaus Neuhaus   +2 more
doaj   +2 more sources

Sperm selection of cryopreserved milt of catfish (Rhamdia quelen) by density gradient centrifugation with AllGrad® 90%

MethodsX, 2021
Density gradient centrifugation is a technique used to wash or separate samples of cryopreserved milt, mainly in humans and bovines allowing, for example, reducing the concentration of cryoprotectants or choosing the best portion of sperm.
Maritza Pérez-Atehortúa, Andrea Galuppo, Rômulo Batista Rodrigues, Nathalia dos Santos Teixeira, Thaiza Rodrigues de Freitas, Thales de Souza França, Lis Santos Marques, Itamar Cossina Gomes, Danilo Pedro Streit, Jr.   +8 more
doaj   +1 more source

#353 : Microfluidic Preparation of Spermatozoa: A Potential Approach in Assisted Reproductive Technology

Fertility & Reproduction, 2023
Background and Aims: While density gradient centrifugation (DGC) is a conventional method in sperm preparation, this method can induce reactive oxygen species (ROS) which cause sperm dysfunction, sperm DNA damage, a recent advancement in sperm processing
Nguyet Minh Ho, Thuy Huong Do, Sy Hung Ho, Manh Ha Nguyen   +3 more
doaj   +1 more source

Percoll discontinuous density gradient centrifugation method for the fractionation of the subpopulations of Mycobacterium smegmatis and Mycobacterium tuberculosis from in vitro cultures

MethodsX, 2023
Bacterial populations in the in vitro laboratory cultures, environment, and patients contain metabolically different subpopulations that respond differently to stress agents, including antibiotics, and emerge as stress tolerant or resistant strains.
Srinivasan Vijay, Rashmi Ravindran Nair, Deepti Sharan, Kishor Jakkala, Parthasarathi Ajitkumar   +4 more
doaj   +1 more source

Variability in Sperm DNA Fragmentation in Men with Mild/Unexplained Subfertility in a Prospective Longitudinal Intrauterine Insemination Trial

Life, 2022
The biological variability of semen and sperm DNA fragmentation (SDF) parameters in a longitudinal intrauterine insemination (IUI) trial over multiple IUI cycles was investigated.
Usha Punjabi, Ella Roelant, Kris Peeters, Ilse Goovaerts, Helga Van Mulders, Diane De Neubourg   +5 more
doaj   +1 more source

The enriched Y-bearing sperm combined with delayed fixed-time artificial insemination for obtaining male Simmental crossbred offspring [PDF]

Veterinary World, 2022
Background and Aim: The production of male calf beef cattle is an agricultural innovation needed to increase the farm's productivity as a provider of meat sources.
Dewa Ketut Meles, Imam Mustofa, Mas'ud Hariadi, Wurlina Wurlina, Suherni Susilowati, Anny Amaliya, Suparto Suparto, Rimayanti Rimayanti   +7 more
doaj   +1 more source

Density gradient centrifugation compromises bone marrow mononuclear cell yield. [PDF]

PLoS ONE, 2012
Bone marrow mononuclear cells (BMNCs) are widely used in regenerative medicine, but recent data suggests that the isolation of BMNCs by commonly used Ficoll-Paque density gradient centrifugation (DGC) causes significant cell loss and influences graft ...
Claudia Pösel, Karoline Möller, Wenke Fröhlich, Isabell Schulz, Johannes Boltze, Daniel-Christoph Wagner   +5 more
doaj   +1 more source