Results 21 to 30 of about 245,556 (263)

Different Methods for Seminal Plasma Removal and Sperm Selection on the Quality and Fertility of Collared Peccary Sperm

Animals, 2023
Methods for seminal plasma (SP) removal and the selection of collared peccary sperm for fertilization were compared. The experiments evaluated the following: the (I) impact of centrifugation for SP removal before swim-up for sperm selection and (II) a ...
Maria V. O. Santos, Andréia M. Silva, Leonardo V. C. Aquino, Lhara R. M. Oliveira, Samara S. J. Moreira, Moacir F. Oliveira, Alexandre R. Silva, Alexsandra F. Pereira   +7 more
doaj   +1 more source

Preparation of purified peste des petits ruminants (PPR) virus antigen from a local isolate of Bangladesh

Fundamental and Applied Agriculture, 2019
Peste des petits ruminants (PPR) is the number one killer disease of small ruminants. The disease causes severe losses to small ruminant production and is presently considered as one of the major threats to about 22 million small ruminant population of ...
Ashrafun Naher Luna, Fardina Sultana Sumi, Jahan Ara Begum, Md. Mushfiqur Rahman, Mohammad Rafiqul Islam, Emdadul Haque Chowdhury   +5 more
doaj   +1 more source

Purification of Pox Viruses by Density Gradient Centrifugation [PDF]

Journal of General Microbiology, 1962
SUMMARY: Rabbit pox virus grown in tissue culture cells and vaccinia virus grown on rabbit skin have been purified as follows. The virus was sedimented and washed once by centrifugation to provide a tenfold virus concentrate. This semi-purified material was layered on top of a sucrose density gradient and centrifuged to produce a zone of virus ...
H T, ZWARTOUW, J C, WESTWOOD, G, APPLEYARD   +2 more
openaire   +2 more sources

In vitro cultivation of rat bone marrow mesenchymal stem cells and establishment of pEGFP/Ang-1 transfection method

Asian Pacific Journal of Tropical Biomedicine, 2014
Objective: To obtain the bone marrow mesenchymal stem cells (BMSCs), complete phenotypic identification and successfully transfect rat BMSCs by recombinant plasmid pEGFP/Ang-1.
Xiu-Qun Zhang, Long Wang, Shu-Li Zhao, Wei Xu   +3 more
doaj   +1 more source

Sperm Selection and Embryo Development: A Comparison of the Density Gradient Centrifugation and Microfluidic Chip Sperm Preparation Methods in Patients with Astheno-Teratozoospermia

Life, 2021
In recent years, microfluidic chip-based sperm sorting has emerged as an alternative tool to centrifugation-based conventional techniques for in vitro fertilization.
Cagla Guler, Sureyya Melil, Umit Ozekici, Yaprak Donmez Cakil, Belgin Selam, Mehmet Cincik   +5 more
doaj   +1 more source

Purification of functional mouse skeletal muscle mitochondria using percoll density gradient centrifugation

BMC Research Notes, 2023
Objective Our goal was to isolate purified mitochondria from mouse skeletal muscle using a Percoll density gradient and to assess bioenergetic function and purity via Seahorse Extracellular Flux (XF) Analyses and mass spectrometry.
Rea Victoria P. Anunciado-Koza, Anyonya R. Guntur, Calvin P. Vary, Carlos A. Gartner, Madeleine Nowak, Robert A. Koza   +5 more
doaj   +1 more source

Timing of semen cryopreservation: before or after processing? [PDF]

Revista Brasileira de Ginecologia e Obstetrícia
Objective: Seminal cryopreservation causes significant damage to the sperm; therefore, different methods of cryopreservation have been studied. The aim of the study was to compare the effects of density gradient processing and washing/centrifugation with
Ana Paula de Souza Kussler, Ivan Cunha Bustamante Filho, Elisa Negri, Edison Capp, Helena von Eye Corleta   +4 more
doaj   +1 more source

Elimination of bacteria from semen using a combination of density gradient centrifugation and swim-up

Journal of Men's Health, 2022
Background: To evaluate the efficiency of bacterial clearance of different sperm preparation procedure. Methods: A total of 46 semen samples were collected in this study, each semen sample was divided into four equal parts (0.5 mL each).
Zhiheng Chen, Lingxiao Jiang, Hui Wang, Wumin Quan, Li Yang, Chunquan Ou, Ling Sun   +6 more
doaj   +1 more source

Swim‐up method is superior to density gradient centrifugation for preserving sperm DNA integrity during sperm processing

Reproductive Medicine and Biology
Purpose This study aimed to evaluate the effects of swim‐up and density gradient centrifugation methods on sperm DNA fragmentation. Methods Nineteen normozoospermic patient samples with ≥100 × 106 motile sperms were included in this study.
Kenji Amano, Satoko Oigawa, Koichiro Ichizawa, Yukiko Tokuda, Mami Unagami, Mami Sekiguchi, Mayuko Furui, Kentaro Nakaoka, Ayumu Ito, Rika Hayashi, Yuko Tamaki, Yuko Hayashi, Yusuke Fukuda, Yukiko Katagiri, Masahiko Nakata, Koichi Nagao   +15 more
doaj   +1 more source

Rab14 regulates the transport of human papillomavirus to the trans‐Golgi network for infectious cell entry

FEBS Letters, EarlyView.
This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley   +1 more source