Results 21 to 30 of about 379,070 (309)

Studies on Rat Peritoneal Mast Cells 1. Purification of Mast Cells From Rat Peritoneal Cavity [PDF]

open access: yes, 1982
To get a high recovery rate of mast cells from the rat peritoneal cavity, a new modified method was discussed using BSA density gradient. Three procedures, peritoneal lavage, washing of peritoneal cells and purification by BSA, were tested in this study,
Kimura, Ikuro   +6 more
core   +1 more source

Different Methods for Seminal Plasma Removal and Sperm Selection on the Quality and Fertility of Collared Peccary Sperm

open access: yesAnimals, 2023
Methods for seminal plasma (SP) removal and the selection of collared peccary sperm for fertilization were compared. The experiments evaluated the following: the (I) impact of centrifugation for SP removal before swim-up for sperm selection and (II) a ...
Maria V. O. Santos   +7 more
doaj   +1 more source

Mitochondrial ribosome assembly in Neurospora. Structural analysis of mature and partially assembled ribosomal subunits by equilibrium centrifugation in CsCl gradients [PDF]

open access: yes, 1982
In Neurospora, one protein associated with the mitochondrial small ribosomal subunit (S-5, Mr 52,000) is synthesized intramitochondrially and is assumed to be encoded by mtDNA.
Lambowitz, Alan M., LaPolla, Robert J.
core   +1 more source

Preparation of purified peste des petits ruminants (PPR) virus antigen from a local isolate of Bangladesh

open access: yesFundamental and Applied Agriculture, 2019
Peste des petits ruminants (PPR) is the number one killer disease of small ruminants. The disease causes severe losses to small ruminant production and is presently considered as one of the major threats to about 22 million small ruminant population of ...
Ashrafun Naher Luna   +5 more
doaj   +1 more source

Mitochondrial Translation Products before and after Integration into the Mitochondrial Membrane in Neurospora crassa [PDF]

open access: yes, 1972
# 1. Nascent translation products on mitochondrial ribosomes were selectively labeled in vivo in the presence of cycloheximide with radioactive leucine. They were isolated together with the ribosomes. # 2.
Michel, Rainer, Neupert, Walter
core   +1 more source

Functional and Biogenetical Heterogeneity of the Inner Membrane of Rat-Liver Mitochondria [PDF]

open access: yes, 1971
Rat liver mitochondria were fragmented by a combined technique of swelling, shrinking, and sonication. Fragments of inner membrane were separated by density gradient centrifugation.
Allmann D. W.   +50 more
core   +1 more source

Distribution of chromaffin secretory vesicles, acetylcholinesterase, and lysosomal enzymes in sucrose and percoll gradients [PDF]

open access: yes, 1984
Crude chromaffin secretory vesicles, obtained by differential centrifugation, were further purified on isotonic (Percoll) gradients. The chromaffin vesicle fractions recovered from the gradients contain acetylcholinesterase as well as lysosomal enzymes ...
Bowers   +31 more
core   +1 more source

Transport of Proteins into Mitochondria [PDF]

open access: yes, 1980
The mitochondrial ADP/ATP carrier is an integral transmembrane protein of the inner membrane. It is synthesized on cytoplasmic ribosomes. Kinetic data suggested that this protein is transferred into mitochondria in a posttranslational manner.
Allen E. H.   +41 more
core   +1 more source

Sperm Selection and Embryo Development: A Comparison of the Density Gradient Centrifugation and Microfluidic Chip Sperm Preparation Methods in Patients with Astheno-Teratozoospermia

open access: yesLife, 2021
In recent years, microfluidic chip-based sperm sorting has emerged as an alternative tool to centrifugation-based conventional techniques for in vitro fertilization.
Cagla Guler   +5 more
doaj   +1 more source

Stable isotope probing: Technical considerations when resolving ¹⁵N-labeled RNA in gradients [PDF]

open access: yes, 2010
RNA based stable isotope probing (SIP) facilitates the detection and identification of active members of microbial populations that are involved in the assimilation of an isotopically labeled compound.
Addison, Sarah Louise   +2 more
core   +2 more sources

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