Results 21 to 30 of about 240,760 (305)

Density-gradient centrifugation analysis of proteins included in the plasma sEV fraction.

, 2022
The sEV fractions were prepared by ultracentrifugation from mouse plasma and further fractionated by Optiprep density-gradient centrifugation, which were subjected to Western blotting using antibodies against CD63, CD9 and GPIIIa. (EPS)
Akiko Takeda (31405), Hideki Mochizuki (204792), Xiaoman Zhang (1885492), Toshihide Takeuchi (115925), Yoshitaka Nagai (115940)   +4 more
core   +1 more source

An Advanced Protocol for the Quantification of Marine Sediment Viruses via Flow Cytometry

Viruses, 2021
Viruses are highly abundant, diverse, and active components of marine environments. Flow cytometry has helped to increase the understanding of their impact on shaping microbial communities and biogeochemical cycles in the pelagic zone.
Mara Elena Heinrichs, Daniele De Corte, Bert Engelen, Donald Pan   +3 more
doaj   +1 more source

Efficacy of density gradient centrifugation technique (DGC) in enhancing sperm cell DNA quality for assisted reproductive technique

Middle East Fertility Society Journal, 2022
Objective Density gradient centrifugation (DGC) is commonly used for sperm preparation before assisted reproductive technology (ART) procedures. This technique separates superior motile spermatozoa with normal morphology from the total sperm population ...
Assila Hadj Ali, Tesnim Ajina, Maroua Ben Ali, Meriem Mehdi   +3 more
doaj   +1 more source

Isolation of mitochondria from Saccharomyces cerevisiae using magnetic bead affinity purification. [PDF]

PLoS ONE, 2018
Isolated mitochondria are widely used to study the function of the organelle. Typically, mitochondria are prepared using differential centrifugation alone or in conjunction with density gradient ultracentrifugation.
Pin-Chao Liao, Istvan R Boldogh, Stephanie E Siegmund, Zachary Freyberg, Liza A Pon   +4 more
doaj   +1 more source

Pemisahan Spermatozoa Berkromosom X dan Y Kambing Boer dan Aplikasinya Melalui Inseminasi Buatan Untuk Mendapatkan Jenis Kelamin Anak Sesuai Harapan

Jurnal Agripet, 2013
Separation of spermatozoa with x and y chromosome at boer goat and its application by artificial insemination for kid sex purpose ABSTRACT. The purposes of this experiment are to investigate the separation of X and Y spermatozoa by measuring the ...
Dasrul Dasrul, M. Aman Yaman, Zulfan Zulfan   +2 more
doaj   +1 more source

Split Nano Luciferase-based Assay to Measure Assembly of Japanese Encephalitis Virus

Bio-Protocol, 2020
Cells infected with flavivirus release various forms of infectious and non-infectious particles as products and by-products. Comprehensive profiling of the released particles by density gradient centrifugation is informative for understanding viral ...
Simon Goto, Kotaro Ishida, Ryosuke Suzuki, Eiji Morita   +3 more
doaj   +1 more source

Preparation of purified peste des petits ruminants (PPR) virus antigen from a local isolate of Bangladesh

Fundamental and Applied Agriculture, 2019
Peste des petits ruminants (PPR) is the number one killer disease of small ruminants. The disease causes severe losses to small ruminant production and is presently considered as one of the major threats to about 22 million small ruminant population of ...
Ashrafun Naher Luna, Fardina Sultana Sumi, Jahan Ara Begum, Md. Mushfiqur Rahman, Mohammad Rafiqul Islam, Emdadul Haque Chowdhury   +5 more
doaj   +1 more source

Different Methods for Seminal Plasma Removal and Sperm Selection on the Quality and Fertility of Collared Peccary Sperm

Animals, 2023
Methods for seminal plasma (SP) removal and the selection of collared peccary sperm for fertilization were compared. The experiments evaluated the following: the (I) impact of centrifugation for SP removal before swim-up for sperm selection and (II) a ...
Maria V. O. Santos, Andréia M. Silva, Leonardo V. C. Aquino, Lhara R. M. Oliveira, Samara S. J. Moreira, Moacir F. Oliveira, Alexandre R. Silva, Alexsandra F. Pereira   +7 more
doaj   +1 more source

In vitro cultivation of rat bone marrow mesenchymal stem cells and establishment of pEGFP/Ang-1 transfection method

Asian Pacific Journal of Tropical Biomedicine, 2014
Objective: To obtain the bone marrow mesenchymal stem cells (BMSCs), complete phenotypic identification and successfully transfect rat BMSCs by recombinant plasmid pEGFP/Ang-1.
Xiu-Qun Zhang, Long Wang, Shu-Li Zhao, Wei Xu   +3 more
doaj   +1 more source

Sperm Selection and Embryo Development: A Comparison of the Density Gradient Centrifugation and Microfluidic Chip Sperm Preparation Methods in Patients with Astheno-Teratozoospermia

Life, 2021
In recent years, microfluidic chip-based sperm sorting has emerged as an alternative tool to centrifugation-based conventional techniques for in vitro fertilization.
Cagla Guler, Sureyya Melil, Umit Ozekici, Yaprak Donmez Cakil, Belgin Selam, Mehmet Cincik   +5 more
doaj   +1 more source