Results 31 to 40 of about 379,070 (309)

In vitro cultivation of rat bone marrow mesenchymal stem cells and establishment of pEGFP/Ang-1 transfection method

Asian Pacific Journal of Tropical Biomedicine, 2014
Objective: To obtain the bone marrow mesenchymal stem cells (BMSCs), complete phenotypic identification and successfully transfect rat BMSCs by recombinant plasmid pEGFP/Ang-1.
Xiu-Qun Zhang, Long Wang, Shu-Li Zhao, Wei Xu   +3 more
doaj   +1 more source

Distinct Intracellular Compartments Involved in Invariant Chain Degradation and Antigenic Peptide Loading of Major Histocompatibility Complex (MHC) Class II Molecules [PDF]

, 1997
Major histocompatibility complex (MHC) class II molecules are transported to intracellular MHC class II compartments via a transient association with the invariant chain (Ii).
Ferrari, G., Knight, A. M., Watts, C., Pieters, J.   +3 more
core   +1 more source

Motility fractionation of bacteria by centrifugation

, 2013
Centrifugation is a widespread laboratory technique used to separate mixtures into fractions characterized by a specific size, weight or density. We demonstrate that centrifugation can be also used to separate swimming cells having different motility. To
Di Leonardo, Roberto, Lepore, Alessia, Maggi, Claudio, Rizzo, Alessandro, Solari, Jacopo   +4 more
core   +1 more source

Minimal Information About Sample Preparation for Phosphoproteomics [PDF]

, 2009
This guideline describes parameters and conditions involved in phosphopeptide sample preparation. It covers from the description and preparation of the cells and tissues to the fractionation and specific enrichment of phosphopeptides for MS analysis. The
David Ovelleiro, Joaquin Abian, Marina Gay, Montserrat Carrascal   +3 more
core   +2 more sources

Purification of functional mouse skeletal muscle mitochondria using percoll density gradient centrifugation

BMC Research Notes, 2023
Objective Our goal was to isolate purified mitochondria from mouse skeletal muscle using a Percoll density gradient and to assess bioenergetic function and purity via Seahorse Extracellular Flux (XF) Analyses and mass spectrometry.
Rea Victoria P. Anunciado-Koza, Anyonya R. Guntur, Calvin P. Vary, Carlos A. Gartner, Madeleine Nowak, Robert A. Koza   +5 more
doaj   +1 more source

Elimination of bacteria from semen using a combination of density gradient centrifugation and swim-up

Journal of Men's Health, 2022
Background: To evaluate the efficiency of bacterial clearance of different sperm preparation procedure. Methods: A total of 46 semen samples were collected in this study, each semen sample was divided into four equal parts (0.5 mL each).
Zhiheng Chen, Lingxiao Jiang, Hui Wang, Wumin Quan, Li Yang, Chunquan Ou, Ling Sun   +6 more
doaj   +1 more source

Comparative proteomic analysis of spermatozoa isolated by swim-up or density gradient centrifugation [PDF]

, 2015
BACKGROUND: Reports about the morphologic and functional characteristics of spermatozoa prepared by density gradient centrifugation (DC) or swim-up (SU) have produced discordant results.
A Ashrafzadeh, A Hamada, AB Siva, AC Williams, AL Monqaut, BT Storey, C Frapsauce, CW Woschnagg, DC Ding, E Borges Jr, E Chantler, E Goldberg, Elena Giolo, Elisa Giacomini, F Sapienza, F Secciani, G Candiano, G Ricci, G Ricci, Gabriella Zito, Giuseppe Ricci, H Margaryan, J Martinez-Heredia, J Martinez-Heredia, JC Nishihara, JE Welch, K Miki, KA Jaroudi, KL Pixton, L Lefievre, LW Li, M Fraczek, ME Hammadeh, Monica Martinelli, N Tanphaichitr, P Prakash, P Van der Zwalmen, P Vigil, PV Hornbeck, R Sharma, RJ Aitken, Rodolfo C Garcia, RR Henkel, S Beranova-Giorgianni, S De Mateo, S Lopes, S McReynolds, S Saha, SE Kim, SE Ong, Stefania Luppi, T Rabilloud, T-T Liao, TH Hereng, U Paasch, WHO, X Xue   +56 more
core   +2 more sources

Timing of semen cryopreservation: before or after processing? [PDF]

Revista Brasileira de Ginecologia e Obstetrícia
Objective: Seminal cryopreservation causes significant damage to the sperm; therefore, different methods of cryopreservation have been studied. The aim of the study was to compare the effects of density gradient processing and washing/centrifugation with
Ana Paula de Souza Kussler, Ivan Cunha Bustamante Filho, Elisa Negri, Edison Capp, Helena von Eye Corleta   +4 more
doaj   +1 more source

Sorting and transmission electron microscopy analysis of single or double wall carbon nanotubes [PDF]

, 2009
On the basis of the recent progress on the sorting of carbon nanotubes’ structure with respect to their diameter or number of walls, we investigate by transmission electron microscopy the sorting efficiency, with a comparison with optical absorption ...
Arnold, Avouris, Bachilo, Flahaut, Fleurier, Green, Hertel, Jiang, Kim, Lambin, Lauret, Lauret, O'Connell, Rao, Sato   +14 more
core   +2 more sources

Sites of Biosynthesis of Outer and Inner Membrane Proteins of Neurospora crassa Mitochondria [PDF]

, 1970
Outer and inner membranes of Neurospora crassa mitochondria were separated by the combined swelling, shrinking, sonication procedure. Membranes were characterized by electron microscopy and by marker enzyme activities.
Aasen A. J., Arrigoni O., Beattie D. S., Beattie D. S., Beisenherz G., Cassady W. E., Goodwin T. W., Harding R. W., Hawley E. S., Neupert W., Parsons D., Schnaitman G., Schott H.-H., Vogel H. J., Weiss H.   +14 more
core   +1 more source