Results 31 to 40 of about 240,760 (305)

Rapid separation of microalgae by density gradient centrifugation in percoll

, 1983
A simple and rapid technique is described for the separation of different microalgae from mixed cultures. The technique relies upon density gradient centrifugation in the silica sol Percoll, thus separation is achieved on the basis of differences in ...
Codd, Geoffrey A., Lanaras, Thomas, Whitelam, Garry C.   +2 more
core   +1 more source

Single-cell mass-density measurements using microchannel gradient centrifugation

, 2022
We present a microchannel-based adaption of density-gradient centrifugation that allows the instantaneous, precise, and repeatable generation of a 1-dimensional mass-density gradient and determination of single-cell densities with integrated mass-density
Soller, Richard, Barnkob, Rune
core  

Purification of functional mouse skeletal muscle mitochondria using percoll density gradient centrifugation

BMC Research Notes, 2023
Objective Our goal was to isolate purified mitochondria from mouse skeletal muscle using a Percoll density gradient and to assess bioenergetic function and purity via Seahorse Extracellular Flux (XF) Analyses and mass spectrometry.
Rea Victoria P. Anunciado-Koza, Anyonya R. Guntur, Calvin P. Vary, Carlos A. Gartner, Madeleine Nowak, Robert A. Koza   +5 more
doaj   +1 more source

Goat Sperm Sexing by Percoll Density Gradient Centrifugation [PDF]

, 2020
วิทยานิพนธ์ (วท.ม. (สัตวศาสตร์))--มหาวิทยาลัยสงขลานครินทร์, 2563The objectives of this study were to investigate the post-thawed semen quality and sex ratio of kids with sperm sexed by Percoll density gradient centrifugation in goat.
วิศิษฐ ทองเที่ยง
core  

Timing of semen cryopreservation: before or after processing? [PDF]

Revista Brasileira de Ginecologia e Obstetrícia
Objective: Seminal cryopreservation causes significant damage to the sperm; therefore, different methods of cryopreservation have been studied. The aim of the study was to compare the effects of density gradient processing and washing/centrifugation with
Ana Paula de Souza Kussler, Ivan Cunha Bustamante Filho, Elisa Negri, Edison Capp, Helena von Eye Corleta   +4 more
doaj   +1 more source

Purification of Pox Viruses by Density Gradient Centrifugation [PDF]

Journal of General Microbiology, 1962
SUMMARY: Rabbit pox virus grown in tissue culture cells and vaccinia virus grown on rabbit skin have been purified as follows. The virus was sedimented and washed once by centrifugation to provide a tenfold virus concentrate. This semi-purified material was layered on top of a sucrose density gradient and centrifuged to produce a zone of virus ...
H T, ZWARTOUW, J C, WESTWOOD, G, APPLEYARD   +2 more
openaire   +2 more sources

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

Treatment of human sperm with serine protease during density gradient centrifugation [PDF]

, 2012
PURPOSE Seminal pathogens can bind specifically or nonspecifically to spermatozoa, rendering semen decontamination procedures ineffective, whereby vertical or horizontal transmission of the infection could occur.
Huyser, Carin, Fourie, Jozef Markus, Loskutoff, N.   +2 more
core   +1 more source

Organ‐specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

FEBS Letters, EarlyView.
We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley   +1 more source

Reduced senescence and retained nuclear DNA integrity in human spermatozoa prepared by density gradient centrifugation

, 2004
Purpose: To investigate whether removal of extraneous cells and immotile spermatozoa from a sperm preparation by density gradient centrifugation could help to maintain normal spermatozoa in a viable state and retain their deoxyribonucleic acid integrity.
D. Sakkas, BIZZARO, Davide, H. Nilsson, MORRELL JM, MANICARDI GC, D. Bizzaro, PV Holmes, O. Moffatt, M. Tomlinson, MANICARDI, Gian Carlo, SAKKAS D, TOMLINSON M, NILSSON H, MOFFATT O, JM Morrell, HOLMES P.V.   +15 more
core   +1 more source