Results 41 to 50 of about 245,556 (263)

Ligand‐dependent transcriptional heterogeneity in cell cycle gene expression delays G1/S entry

FEBS Letters, EarlyView.
EGF and HRG induce distinct G1/S progression programs in ErbB2‐amplified BT474 breast cancer cells. Despite activating the potent ErbB2–ErbB3 heterodimer, HRG does not accelerate cell‐cycle entry. Instead, EGF promotes earlier restriction‐point passage via ERK–FOS signaling, whereas HRG activates the AKT–MYC axis, driving transcriptional heterogeneity ...
Ririn Rahmala Febri, Ayaka Nagasato‐Ichikawa, Keita Iida, Shuhei Kimura, Akira Imamoto, Mariko Okada   +5 more
wiley   +1 more source

PeranSenyawa Oksigen Reaktif dalam Makanisme KerusakanIntegritas Membran Spermatozoa KerbauLumpur Setelah Sentrifugasi Gradien Densitas Percoll

JKS (Jurnal Kedokteran Syiah Kuala), 2008
Abstrak.  Penelitian  ini  bertujuan  untuk  menganalisa senyawa  oksigen  reaktif  dan  pengaruhnya  terhadap integritas membran plasma spermatozoa kerbau lumpur basil sentrifugasi gradien densitas percoll.
Dasrul Dasrul, Hardjopranjot Hardjopranjot, Mahaputra L, Sudjarwo4 Sudjarwo4   +3 more
doaj  

A density gradient centrifugation method for rapid separation of nanoTiO2 and TiO2 aggregates from microalgal cells in complex mixtures with mercury

MethodsX, 2020
In natural environment, the microorganisms are exposed to complex mixtures of contaminants, including manufactured nanoparticles and their aggregates.
Mengting Li, Vera I. Slaveykova
doaj   +1 more source

Structures of mycobacterial 3‐methylcrotonyl‐CoA carboxylase reveal carrier‐domain translocation between catalytic sites

FEBS Letters, EarlyView.
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav, Bogdan I. Florea, Sebastian Geibel   +2 more
wiley   +1 more source

Density Gradient Centrifugation for Enrichment and Identification of GFP-tagged Chitosomal Microvesicles of Filamentous Fungi

Bio-Protocol, 2015
Density gradient centrifugation has been utilized to characterize the subcellular distribution of physiologically relevant enzymes in yeasts and filamentous fungi (Leal-Morales et al., 1988; Martínez et al., 1989; Kamada et al., 1991).
Jorge Verdín, Eddy Sánchez-León, Rosa Fajardo-Somera, Carlos Morales, Salomón Bartnicki-García, Meritxell Riquelme   +5 more
doaj   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

COMPARISON BETWEEN TWO-LAYER DENSITY GRADIENT AND THREE-LAYER DENSITY GRADIENT TECHNIQUE FOR SPERM PREPARATION AT ASTER FERTILITY CLINIC, DR. HASAN SADIKIN GENERAL HOSPITAL

International Journal of Integrated Health Sciences, 2014
Objective: To compare the efficacy of sperm preparation techniques modified by two layer density gradient technique (80%, 40%). Methods: Sperm preparation was performed by two- and three-layer density gradient technique by centrifugation at 500 rpm ...
Nanang Winarto Astarto, Dian Tjahyadi, Sintya Jatnikasari   +2 more
doaj   +1 more source

Leucine‐rich glioma inactivated 1 (LGI1) is a ganglioside‐binding protein

FEBS Letters, EarlyView.
Neuronal hyperexcitability associated with a decrease/absence of the extracellular protein LGI1 has been suggested to be primarily due to the downregulation of Kv1 channel expression. The molecular mechanisms underlying this decrease have not yet been elucidated.
Kévin Debreux, Christian Lévêque, Fodil Azzaz, Marion Sangiardi, Sarosh R Irani, Michael Seagar, Jacques Fantini, Oussama El Far   +7 more
wiley   +1 more source

Effects of Semen Processing on Sperm Function: Differences between Swim-Up and Density Gradient Centrifugation

The World Journal of Men's Health, 2021
Purpose: Andrology research has evolved notoriously in the latest years, particularly since male factor contribution to couple infertility has been undoubtedly demonstrated.
Gabriela Hernández-Silva, Aideé S. López-Torres, Israel Maldonado-Rosas, Esperanza Mata-Martínez, Fernando Larrea, Víctor Torres-Flores, Claudia L. Treviño, Mayel Chirinos   +7 more
doaj   +1 more source

IMPDH inhibition enhances cytarabine efficacy in SAMHD1‐expressing leukaemia cells via guanine nucleotide depletion

Molecular Oncology, EarlyView.
Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla, Christopher Dirks, Caroline Eiden, Sonja K. Fesenmayer, Femke M. Hormann, Yolande Klootsema, Ingrid Lilienthal, Si Min Zhang, Nikolas Herold, Sean G. Rudd   +9 more
wiley   +1 more source