Results 41 to 50 of about 240,760 (305)

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

IMPACT OF PREPARATION USING CONVENTIONAL AND MODIFIED DENSITY GRADIENT CENTRIFUGATION METHODS ON SPERM CONCENTRATION, MOTILITY AND NUMBER OF NORMAL MOTILE SPERM RECOVERY (NMSR)

Folia Medica Indonesiana, 2017
Some preparation techniques, either conventional or advanced, have been provided. Advanced technique may overcome the limitations of conventional techniques.
Rina Yudiwati, MPBD Pramesti, Agustinus Agustinus, E Pradana, Bambang Purwanto   +4 more
doaj   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Ion-exchange chromatography purification of extracellular vesicles

BioTechniques, 2017
Despite numerous studies, isolating pure preparations of extracellular vesicles (EVs) has proven challenging. Here, we compared ion-exchange chromatography (IEC) to the widely used sucrose density gradient (SDG) centrifugation method for the purification
Maja Kosanović, Bojana Milutinović, Sanja Goč, Ninoslav Mitić, Miroslava Janković   +4 more
doaj   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Red blood cell layers after Percoll density gradient centrifugation.

, 2015
Seven RBC fractions were generated by Percoll density gradient centrifugation. Least dense fraction on the top consisted of youngest RBC; increasing density indicated increasing RBC age.
Daniel Alexander Bizjak (728303), Marijke Grau (284060), Christian Brinkmann (728304), Wilhelm Bloch (45980)   +3 more
core   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

PeranSenyawa Oksigen Reaktif dalam Makanisme KerusakanIntegritas Membran Spermatozoa KerbauLumpur Setelah Sentrifugasi Gradien Densitas Percoll

JKS (Jurnal Kedokteran Syiah Kuala), 2008
Abstrak.  Penelitian  ini  bertujuan  untuk  menganalisa senyawa  oksigen  reaktif  dan  pengaruhnya  terhadap integritas membran plasma spermatozoa kerbau lumpur basil sentrifugasi gradien densitas percoll.
Dasrul Dasrul, Hardjopranjot Hardjopranjot, Mahaputra L, Sudjarwo4 Sudjarwo4   +3 more
doaj  

A density gradient centrifugation method for rapid separation of nanoTiO2 and TiO2 aggregates from microalgal cells in complex mixtures with mercury

MethodsX, 2020
In natural environment, the microorganisms are exposed to complex mixtures of contaminants, including manufactured nanoparticles and their aggregates.
Mengting Li, Vera I. Slaveykova
doaj   +1 more source