Results 61 to 70 of about 240,760 (305)
FEBS Letters, EarlyView.We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
Bio-Protocol, 2013 Peripheral blood mononuclear cells (PBMCs) are chiefly lymphocytes and monocytes. PBMCs are separated from the whole blood by a density gradient centrifugation method using Ficoll Histopaque.
Santosh Panda, Balachandran Ravindran
doaj +1 more source
, 2014 OBJECTIVE: We evaluated the effectiveness of sperm washing using the discontinuous two-layer density gradient centrifugation method to remove antisperm antibodies attached to the sperm surface.
Esteves,Sandro +3 more
core +1 more source
Molecular Oncology, EarlyView.Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla +9 more
wiley +1 more source
Molecular Oncology, EarlyView.We have established a humanized orthotopic patient‐derived xenograft (Hu‐oPDX) mouse model of high‐grade serous ovarian cancer (HGSOC) that recapitulates human tumor–immune interactions. Using combined anti‐PD‐L1/anti‐CD73 immunotherapy, we demonstrate the model's improved biological relevance and enhanced translational value for preclinical ...
Luka Tandaric +10 more
wiley +1 more source
Effect of Density Gradient Centrifugation on Quality and Recovery Rate of Equine Sperm [PDF]
, 2009 Density gradient centrifugation of sperm is a common assisted-reproduction procedure in humans used to improve semen quality. The technique allows sperm separation based on their isopycnic points.
Edmond, Ann J.
core
CCDC80 suppresses high‐grade serous ovarian cancer migration via negative regulation of B7‐H3
Molecular Oncology, EarlyView.PAX8 is a lineage‐specific master regulator of transcription in high‐grade serous ovarian cancer (HGSC) progression. We show for the first time that PAX8 facilitates proliferation and metastasis by repressing the cell autonomous tumor suppressor CCDC80 and inducing B7‐H3 expression.
Aya Saleh +12 more
wiley +1 more source
Bio-Protocol, 2013 Membrane preparation has been widely used for characterization the membrane proteins. Membrane fractions can be separated by a combination of differential and density-gradient centrifugation techniques (Hodges et al., 1972; Leonard and Vanderwoude, 1976).
Haibing Yang, Angus Murphy
doaj +1 more source
Application of Percoll density gradient centrifugation for separation of Babesia ovatainfected erythrocytes [PDF]
, 2017 application/pdfIn this study, potential of the Percoll density gradient centrifugation method for separation of bovine red bold cells (RBCs) infected with each merozoite type of Babesia ovata was examined.
Tsubokawa, Daigo +8 more
core
Density gradient centrifugation of frozen-thawed equine semen with low motility and fertility.
, 2012 Stallions are not selected for fertility, and breeders can demand the use of frozen semen of high valuable stallions despite a poor quality after thawing.The aim of this study was to verify if density gradient centrifugation of low quality frozenthawed ...
MARI, GAETANO +5 more
core +1 more source