Results 11 to 20 of about 245,556 (263)

Low-Density Granulocyte Contamination From Peripheral Blood Mononuclear Cells of Patients With Sepsis and How to Remove It – A Technical Report

open access: yesFrontiers in Immunology, 2021
Elucidating the mechanisms contributing to the dysregulated host response to infection as part of the syndrome is a current challenge in sepsis research. Peripheral blood mononuclear cells are widely used in immunological studies.
Judith Schenz   +4 more
doaj   +1 more source

The enriched Y-bearing sperm combined with delayed fixed-time artificial insemination for obtaining male Simmental crossbred offspring [PDF]

open access: yesVeterinary World, 2022
Background and Aim: The production of male calf beef cattle is an agricultural innovation needed to increase the farm's productivity as a provider of meat sources.
Dewa Ketut Meles   +7 more
doaj   +1 more source

Comparison of Sperm Telomere Length between Two Sperm Selection Procedures: Density Gradient Centrifugation and Zeta Potential [PDF]

open access: yesInternational Journal of Fertility and Sterility, 2020
Background Telomeres are particular sequences of DNA located at the end of the eukaryotic chromosomes that are essential for genome integrity. Telomere length in spermatozoa differs among males, as well as spermatozoa.
Razieh Ghorbani-sini   +6 more
doaj   +1 more source

An Advanced Protocol for the Quantification of Marine Sediment Viruses via Flow Cytometry

open access: yesViruses, 2021
Viruses are highly abundant, diverse, and active components of marine environments. Flow cytometry has helped to increase the understanding of their impact on shaping microbial communities and biogeochemical cycles in the pelagic zone.
Mara Elena Heinrichs   +3 more
doaj   +1 more source

Density Gradient Centrifugation of Rubella Virus [PDF]

open access: yesJournal of Virology, 1968
Rubella virus was centrifuged in sucrose density gradients. One of two densities could be ascribed to the virus, depending upon the suspending medium used. The virus was found at a density of 1.16 g/cm 3 after centrifugation for 18 hr in sucrose gradients prepared in distilled water.
R M, McCombs, W E, Rawls
openaire   +2 more sources

Efficacy of density gradient centrifugation technique (DGC) in enhancing sperm cell DNA quality for assisted reproductive technique

open access: yesMiddle East Fertility Society Journal, 2022
Objective Density gradient centrifugation (DGC) is commonly used for sperm preparation before assisted reproductive technology (ART) procedures. This technique separates superior motile spermatozoa with normal morphology from the total sperm population ...
Assila Hadj Ali   +3 more
doaj   +1 more source

Isolation of mitochondria from Saccharomyces cerevisiae using magnetic bead affinity purification. [PDF]

open access: yesPLoS ONE, 2018
Isolated mitochondria are widely used to study the function of the organelle. Typically, mitochondria are prepared using differential centrifugation alone or in conjunction with density gradient ultracentrifugation.
Pin-Chao Liao   +4 more
doaj   +1 more source

Pemisahan Spermatozoa Berkromosom X dan Y Kambing Boer dan Aplikasinya Melalui Inseminasi Buatan Untuk Mendapatkan Jenis Kelamin Anak Sesuai Harapan

open access: yesJurnal Agripet, 2013
Separation of spermatozoa with x and y chromosome at boer goat and its application by artificial insemination for kid sex purpose ABSTRACT. The purposes of this experiment are to investigate the separation of X and Y spermatozoa by measuring the ...
Dasrul Dasrul   +2 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane. [PDF]

open access: yesFEBS Open Bio
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Ghosh S, Mukherjee K, Bhattacharyya SN.
europepmc   +2 more sources

Split Nano Luciferase-based Assay to Measure Assembly of Japanese Encephalitis Virus

open access: yesBio-Protocol, 2020
Cells infected with flavivirus release various forms of infectious and non-infectious particles as products and by-products. Comprehensive profiling of the released particles by density gradient centrifugation is informative for understanding viral ...
Simon Goto   +3 more
doaj   +1 more source

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