Results 11 to 20 of about 245,556 (263)
Elucidating the mechanisms contributing to the dysregulated host response to infection as part of the syndrome is a current challenge in sepsis research. Peripheral blood mononuclear cells are widely used in immunological studies.
Judith Schenz +4 more
doaj +1 more source
The enriched Y-bearing sperm combined with delayed fixed-time artificial insemination for obtaining male Simmental crossbred offspring [PDF]
Background and Aim: The production of male calf beef cattle is an agricultural innovation needed to increase the farm's productivity as a provider of meat sources.
Dewa Ketut Meles +7 more
doaj +1 more source
Comparison of Sperm Telomere Length between Two Sperm Selection Procedures: Density Gradient Centrifugation and Zeta Potential [PDF]
Background Telomeres are particular sequences of DNA located at the end of the eukaryotic chromosomes that are essential for genome integrity. Telomere length in spermatozoa differs among males, as well as spermatozoa.
Razieh Ghorbani-sini +6 more
doaj +1 more source
An Advanced Protocol for the Quantification of Marine Sediment Viruses via Flow Cytometry
Viruses are highly abundant, diverse, and active components of marine environments. Flow cytometry has helped to increase the understanding of their impact on shaping microbial communities and biogeochemical cycles in the pelagic zone.
Mara Elena Heinrichs +3 more
doaj +1 more source
Density Gradient Centrifugation of Rubella Virus [PDF]
Rubella virus was centrifuged in sucrose density gradients. One of two densities could be ascribed to the virus, depending upon the suspending medium used. The virus was found at a density of 1.16 g/cm 3 after centrifugation for 18 hr in sucrose gradients prepared in distilled water.
R M, McCombs, W E, Rawls
openaire +2 more sources
Objective Density gradient centrifugation (DGC) is commonly used for sperm preparation before assisted reproductive technology (ART) procedures. This technique separates superior motile spermatozoa with normal morphology from the total sperm population ...
Assila Hadj Ali +3 more
doaj +1 more source
Isolation of mitochondria from Saccharomyces cerevisiae using magnetic bead affinity purification. [PDF]
Isolated mitochondria are widely used to study the function of the organelle. Typically, mitochondria are prepared using differential centrifugation alone or in conjunction with density gradient ultracentrifugation.
Pin-Chao Liao +4 more
doaj +1 more source
Separation of spermatozoa with x and y chromosome at boer goat and its application by artificial insemination for kid sex purpose ABSTRACT. The purposes of this experiment are to investigate the separation of X and Y spermatozoa by measuring the ...
Dasrul Dasrul +2 more
doaj +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane. [PDF]
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Ghosh S, Mukherjee K, Bhattacharyya SN.
europepmc +2 more sources
Split Nano Luciferase-based Assay to Measure Assembly of Japanese Encephalitis Virus
Cells infected with flavivirus release various forms of infectious and non-infectious particles as products and by-products. Comprehensive profiling of the released particles by density gradient centrifugation is informative for understanding viral ...
Simon Goto +3 more
doaj +1 more source

