Results 21 to 30 of about 239,867 (289)

Separation of Stabilized MOPS Gold Nanostars by Density Gradient Centrifugation [PDF]

ACS Omega, 2017
Kavita Chandra, Vished Kumar, Stephanie E. Werner, Teri W. Odom   +3 more
doaj   +2 more sources

#353 : Microfluidic Preparation of Spermatozoa: A Potential Approach in Assisted Reproductive Technology

Fertility & Reproduction, 2023
Background and Aims: While density gradient centrifugation (DGC) is a conventional method in sperm preparation, this method can induce reactive oxygen species (ROS) which cause sperm dysfunction, sperm DNA damage, a recent advancement in sperm processing
Nguyet Minh Ho, Thuy Huong Do, Sy Hung Ho, Manh Ha Nguyen   +3 more
doaj   +1 more source

Variability in Sperm DNA Fragmentation in Men with Mild/Unexplained Subfertility in a Prospective Longitudinal Intrauterine Insemination Trial

Life, 2022
The biological variability of semen and sperm DNA fragmentation (SDF) parameters in a longitudinal intrauterine insemination (IUI) trial over multiple IUI cycles was investigated.
Usha Punjabi, Ella Roelant, Kris Peeters, Ilse Goovaerts, Helga Van Mulders, Diane De Neubourg   +5 more
doaj   +1 more source

The enriched Y-bearing sperm combined with delayed fixed-time artificial insemination for obtaining male Simmental crossbred offspring [PDF]

Veterinary World, 2022
Background and Aim: The production of male calf beef cattle is an agricultural innovation needed to increase the farm's productivity as a provider of meat sources.
Dewa Ketut Meles, Imam Mustofa, Mas'ud Hariadi, Wurlina Wurlina, Suherni Susilowati, Anny Amaliya, Suparto Suparto, Rimayanti Rimayanti   +7 more
doaj   +1 more source

Density gradient centrifugation compromises bone marrow mononuclear cell yield. [PDF]

PLoS ONE, 2012
Bone marrow mononuclear cells (BMNCs) are widely used in regenerative medicine, but recent data suggests that the isolation of BMNCs by commonly used Ficoll-Paque density gradient centrifugation (DGC) causes significant cell loss and influences graft ...
Claudia Pösel, Karoline Möller, Wenke Fröhlich, Isabell Schulz, Johannes Boltze, Daniel-Christoph Wagner   +5 more
doaj   +1 more source

Low-Density Granulocyte Contamination From Peripheral Blood Mononuclear Cells of Patients With Sepsis and How to Remove It – A Technical Report

Frontiers in Immunology, 2021
Elucidating the mechanisms contributing to the dysregulated host response to infection as part of the syndrome is a current challenge in sepsis research. Peripheral blood mononuclear cells are widely used in immunological studies.
Judith Schenz, Manuel Obermaier, Sandra Uhle, Markus Alexander Weigand, Florian Uhle   +4 more
doaj   +1 more source

Density-gradient centrifugation analysis of proteins included in the plasma sEV fraction.

, 2022
The sEV fractions were prepared by ultracentrifugation from mouse plasma and further fractionated by Optiprep density-gradient centrifugation, which were subjected to Western blotting using antibodies against CD63, CD9 and GPIIIa. (EPS)
Akiko Takeda (31405), Hideki Mochizuki (204792), Xiaoman Zhang (1885492), Toshihide Takeuchi (115925), Yoshitaka Nagai (115940)   +4 more
core   +1 more source

Density Gradient Centrifugation of Rubella Virus [PDF]

Journal of Virology, 1968
Rubella virus was centrifuged in sucrose density gradients. One of two densities could be ascribed to the virus, depending upon the suspending medium used. The virus was found at a density of 1.16 g/cm 3 after centrifugation for 18 hr in sucrose gradients prepared in distilled water.
R M, McCombs, W E, Rawls
openaire   +2 more sources

An Advanced Protocol for the Quantification of Marine Sediment Viruses via Flow Cytometry

Viruses, 2021
Viruses are highly abundant, diverse, and active components of marine environments. Flow cytometry has helped to increase the understanding of their impact on shaping microbial communities and biogeochemical cycles in the pelagic zone.
Mara Elena Heinrichs, Daniele De Corte, Bert Engelen, Donald Pan   +3 more
doaj   +1 more source

Efficacy of density gradient centrifugation technique (DGC) in enhancing sperm cell DNA quality for assisted reproductive technique

Middle East Fertility Society Journal, 2022
Objective Density gradient centrifugation (DGC) is commonly used for sperm preparation before assisted reproductive technology (ART) procedures. This technique separates superior motile spermatozoa with normal morphology from the total sperm population ...
Assila Hadj Ali, Tesnim Ajina, Maroua Ben Ali, Meriem Mehdi   +3 more
doaj   +1 more source