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The Optimization of Density Gradients for Zonal Centrifugation
Separation Science, 1969Abstract By using established equations and techniques, it is possible to compute sucrose gradients which are optimized to minimize volume expansion of a zone during sedimentation in zonal rotors. These gradients have been tested experimentally, and it has been found that the greatest expansion of the sample zone occurred during the early stage of ...
S. P. Spragg, C. T. Rankin, R. S. Morrod
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An analysis of eukaryotic genomes by density gradient centrifugation
Journal of Molecular Biology, 1976DNA preparations from 25 eukaryotes, ranging from yeast to man, were analyzed in their base composition, sedimentation coefficient, modal and mean buoyant density in CsCl. Four mammalian and two amphibian DNAs were fractionated by preparative Cs 2 SO 4 −Ag + density gradient centrifugation. The CsCl band profile of each fraction was analyzed in terms
Thiery, J, Macaya, G, Bernardi, G
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Isolation of Mammalian Peroxisomes by Density Gradient Centrifugation
2023Sophisticated organelle fractionation strategies were the workhorse of early peroxisome research and led to the characterization of the principal functions of the organelle. However, even in the era of molecular biology and "omics" technologies, they are still of importance to unravel peroxisome-specific proteomes, confirm the localization of still ...
Andreas, Manner, Markus, Islinger
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Density Gradient Centrifugation Studies on Rabies Virus
Journal of Bacteriology, 1966Neurath, A. Robert (The Wistar Institute, Philadelphia, Pa.), Tadeusz J. Wiktor, and Hilary Koprowski . Density gradient centrifugation studies on rabies virus. J. Bacteriol. 92: 102–106.
Tadeusz J. Wiktor +2 more
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Photometric scanning of centrifuged density gradient columns
Analytical Biochemistry, 1963Abstract Results obtained with an apparatus for recording the ultraviolet absorbance at all depths in a centrifuged density gradient column and for collecting consecutive samples are presented. A heavy sucrose solution is forced from a motor-driven syringe into the bottom of a gradient column.
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Density gradient centrifugations of adenovirus-antibody complexes
Archiv f�r die gesamte Virusforschung, 1965Buoyant density sedimentations in CsCl of adenovirus type 5 and virus-antibody complexes has been described. It has been demonstrated that virus which survives a certain dose of antibody is found at densities lower than that of untreated particles suggesting a coating of the virus with antibody.
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Isolation of Endocitic Organelles by Density Gradient Centrifugation
2008Advanced prefractionation strategies, in combination with highly sensitive and accurate mass spectrometers provide powerful means to detect and analyze low abundant proteins on the subcellular and organelle-specific level. Among enrichment techniques, subcellular fractionation has become the most commonly used.
Taras Stasyk +2 more
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The Isolation of Satellite DNA by Density Gradient Centrifugation
2003The term satellite DNA is used for a DNA component that gives a sharp band in a density gradient and can be resolved from the broader main band of DNA in the gradient. The usual gradient material is CsCl in aqueous buffer and the Cs(+) ions form a density gradient in a centrifugal field. DNA in the solution sediments to its isopycnic point. The density
Craig A. Cooney, Harry R. Matthews
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Preparation of polyallomer centrifuge tubes for density gradients
Analytical Biochemistry, 1969Abstract Thin-walled polyallomer centrifuge tubes can easily be prepared to accept linear sucrose gradients, as delivered by conventional gradient formers. They can thus be used with the same facility and achieve the same resolution as cellulose nitrate tubes, for preparative and analytical density gradient centrifugation.
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Isolation of bovine eosinophils by density gradient centrifugation
American Journal of Veterinary Research, 1985SUMMARY A method for the isolation of an enriched population (> 60%) of bovine eosinophils was developed, using density gradient centrifugation. Eosinophils were isolated from peripheral blood by centrifugation on a density gradient medium of sp gr 1.092.
W H, Chambers +3 more
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