Results 71 to 80 of about 246,524 (265)

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Avocado oil extraction processes: method for cold-pressed high-quality edible oil production versus traditional production

Journal of Agricultural Engineering, 2015
Nowadays the avocado fruit (Persea americana Mill.) is widely regarded as an important fruit for its nutritional values, as it is rich in vital human nutrients.
Giacomo Costagli, Matteo Betti
doaj   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

A Smart Centrifuge for Automated Sample Processing with Liquid Handling Robot

Journal of Open Hardware
Recent advances in the development of automation hardware, such as liquid handling robots, offer accelerated and less labor-intensive workflows for material synthesis and diagnostic assays.
Stefan Guldin, Yueyang Gao, Jialei Shi, Helge Wurdemann, Andrew Redfearm, Simon Dawes, Alaric Taylor   +6 more
doaj  

Wireless temperature calibration for medical centrifuge

Sensors International
Based on the complexity and metrological requirements of temperature calibration within the rotor of a medical low-temperature centrifuge, a wireless temperature calibration device for medical centrifuges has been designed and developed.
Tianyu Zhu, Ju Lu, Jian Shen, Jie Liu, Xinqing Xiao   +4 more
doaj   +1 more source

Water absorption of food wheat flour – comparison of direct and indirect methods

Croatian Journal of Food Science and Technology, 2023
The study aims to compare three methods used for the evaluation of water absorption of wheat flour: a) a direct method consisting of a standard procedure on a Farinograph-E device and b) two indirect methods, namely NIR spectroscopy and centrifugation ...
Tibor Sedláček, Ivan Švec
doaj  

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Effect of different speed and time of centrifugations on decontamination of Leptospira spp. cultures from rat’s kidney

Jurnal Teknologi Laboratorium
The centrifugal force in the centrifuge is able to change the surface properties of bacterial cells and the inner structure, including DNA, so that it can damage bacterial cells.
Budi Prasetyo, Arif Mulyanto, Kusnanto Mukti Wibowo, Kurniawan, Farida Dwi Handayani   +4 more
doaj   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source