Results 11 to 20 of about 3,370 (187)

Monitoring structural modulation of redox-sensitive proteins in cells with MS-CETSA

Redox Biology, 2019
Reactive oxygen species (ROS) induce different cellular stress responses but can also mediate cellular signaling. Augmented levels of ROS are associated with aging, cancer as well as various metabolic and neurological disorders.
Wendi Sun, Lingyun Dai, Han Yu, Brenda Puspita, Tianyun Zhao, Feng Li, Justin L. Tan, Yan Ting Lim, Ming Wei Chen, Radoslaw M. Sobota, Daniel G. Tenen, Nayana Prabhu, Pär Nordlund   +12 more
doaj   +5 more sources

A robust CETSA data analysis automation workflow for routine screening

SLAS Discovery
The Cellular Thermal Shift Assay (CETSA) enables the study of protein-ligand interactions in a cellular context. It provides valuable information on the binding affinity and specificity of both small and large molecule ligands in a relevant physiological
Juan Daniel Florez Weidinger, Moritz Pfreundschuh, Diana Zörb, Ada Yee, Stephan Heyse, Felix Bärenz, Stephan Steigele   +6 more
doaj   +3 more sources

Arecoline as a Novel Scaffold Targeting the ATAD2 Bromodomain for Cell Cycle Modulation [PDF]

Pharmaceutics
Background/Objectives: ATPase family AAA domain-containing protein 2 (ATAD2) is an oncogenic chromatin regulator that amplifies E2F/MYC transcriptional programs, yet direct modulators remain scarce. Arecoline (ARE), the primary alkaloid of the areca nut,
Ting-Syuan Lin, Jingting Wan, Jingjin He, Shidong Cui, Yun Huang, Bojian Zhang, Hsi-Yuan Huang, Kexin Zhu, Jihang Chen, Tao Zhang, Shangfu Li, Liao Hu, Yongfei Wang, Hsien-Da Huang, Ping Tang, Yang-Chi-Dung Lin   +15 more
doaj   +2 more sources

Melatonin targets mitochondrial trifunctional enzyme HADHA to improve lipid metabolism in metabolic dysfunction-associated steatotic liver disease [PDF]

Molecular Biomedicine
Metabolic dysfunction-associated steatotic liver disease (MASLD) is a clinicopathological syndrome characterized by abnormal accumulation of fat within hepatocytes, and there is currently no standardized clinical treatment available.
Yongping Zhu, Yanqing Liu, Rui Liu, Junzhe Zhang, Yuqing Meng, Li Liu, Xin Liu, Dandan Liu, Liwei Gu, Linying Zhong, Xianyu Xu, Ying Li, Jinyan Xu, Lingyun Dai, Shengnan Shen, Jigang Wang   +15 more
doaj   +2 more sources

Quercetin attenuates skin inflammation and fibrosis in systemic sclerosis by targeting the RELA/c-Jun axis to suppress th17 cell responses [PDF]

Frontiers in Immunology
BackgroundAberrant Th17 cell activation and interleukin-17 (IL-17) production drive skin inflammation and progressive fibrosis in systemic sclerosis (SSc).
Xiangzhen Kong, Xiangzhen Kong, Xiangzhen Kong, Hiochon Leong, Min Du, Jingyi Yang, Jingzhen Lai, Ziying Dong, Menghua Hu, Yifei Gu, Yexin Li, Qiaorui Tan, Qiaorui Tan, Haiyan Chu, Qingmei Liu, Yanyun Ma, Weihong Xu, Shanji Li, Shanji Li, Yuxiao Chen, Dongdong Chen, Yuanyuan Chen, Wei Lu, Jie Zhu, Jiucun Wang, Jiucun Wang, Jiucun Wang, Jiucun Wang, Lei Wang   +28 more
doaj   +2 more sources

Deep learning based CETSA feature prediction cross multiple cell lines with latent space representation

Scientific Reports, 2023
Mass spectrometry-coupled cellular thermal shift assay (MS-CETSA), a biophysical principle-based technique that measures the thermal stability of proteins at the proteome level inside the cell, has contributed significantly to the understanding of drug ...
Shenghao Zhao, Xulei Yang, Zeng Zeng, Peisheng Qian, Ziyuan Zhao, Lingyun Dai, Nayana Prabhu, Pär Nordlund, Wai Leong Tam   +8 more
doaj   +3 more sources

Integration of multi-omics and network toxicology reveals TLR4-mediated nephrotoxicity induced by arecoline [PDF]

BMC Pharmacology and Toxicology
Background Arecoline, the primary alkaloid in areca nut, induces renal injury, yet its underlying molecular mechanisms remain poorly understood. This study aimed to identify arecoline’s molecular targets and elucidate the subsequent pathological ...
Jixiang Yuan, Lichen Chen, Xilong Wang, Zhibin Jiang, Dian Jin, Yongheng Bai, Xiaodong Pan, Yifu Li, Feihong Lin, Yong Cai   +9 more
doaj   +2 more sources

Crocin protects against smoke-induced chronic obstructive pulmonary disease by regulating AKT1 [PDF]

Frontiers in Pharmacology
BackgroundChronic obstructive pulmonary disease (COPD) is an inflammatory airway disorder characterized by persistent airflow limitation and pathological features such as airway remodeling.
Yuehong Zhu, Yuehong Zhu, Yuntao Jiang, Yuntao Jiang, Jieping Xu, Jieping Xu, Sicheng Yan, Sicheng Yan, Zhihong Ma, Zhihong Ma   +9 more
doaj   +2 more sources

MonoHER selectively enhances the radiotherapy response in p53 wild-type breast cancer via stabilization of p53 [PDF]

Clinical and Translational Radiation Oncology
Background: Radiotherapy is one of the standard treatments for breast cancer, but its efficacy is limited by tumour radioresistance. Radiosensitizers can improve treatment outcomes. MonoHER, a flavonoid derivative, has shown anticancer potential; however,
Chujie Li, Xiaojun Li, Rianne Biemans, Ming Zhang, Ludwig J. Dubois   +4 more
doaj   +2 more sources

Single-cell multiomics data analysis of potential receptors and therapeutic drugs for epilepsy patients comorbid with depression. [PDF]

PLoS ONE
Depression frequently cooccurs with epilepsy (EP) and has become a focus of clinical management, but effective pharmacological interventions remain limited.
Guiqin Bai, Xuerong Zhou, Cheng Xiong, Xi Kang, Ruiqi Huang, Dazhang Bai, Peilin Zhao, Tao Peng, Cheer Muer, Guohui Jiang, Shushan Zhang   +10 more
doaj   +2 more sources