Results 121 to 130 of about 77,168 (243)

New pharmacological prospectives in erectile function [PDF]

, 2009
Erectile dysfunction (ED), defined as the consistent or recurrent inability of a man to attain and/or maintain a penile erection sufficient for sexual activity ), can be caused by a number of different pathophysiological process.
Mitidieri, Emma
core   +1 more source

Critical time window for NO-cGMP-dependent long-term memory formation after one-trial appetitive conditioning

, 2002
The nitric oxide (NO)-cGMP signaling pathway is implicated in an increasing number of experimental models of plasticity. Here, in a behavioral analysis using one-trial appetitive associative conditioning, we show that there is an obligatory requirement ...
Ildiko Kemenes (4460722), George Kemenes (4460416), Richard J Andrew (16061948), Michael O'Shea (146289), Paul R Benjamin (16036673)   +4 more
core  

New biosensors and transgenic mice for multiplex cGMP imaging

British Journal of Pharmacology, EarlyView.
Background and Purpose Cyclic guanosine monophosphate (cGMP) is a versatile second messenger that is important for human (patho‐)physiology and pharmacotherapy. Live‐cell imaging of cGMP with biosensors allows to elucidate its spatiotemporal dynamics in real time under close‐to‐native conditions. However, to monitor two separate cGMP pools or cGMP/cAMP
Markus Wolters, Martin Thunemann, Barbara Birk, Susanne Feil, Viacheslav O. Nikolaev, Moritz Lehners, Robert Feil   +6 more
wiley   +1 more source

PDE4D and PDE3B orchestrate distinct cAMP microdomains in 3T3‐L1 adipocytes

British Journal of Pharmacology, EarlyView.
Basal conditions: •Ins/PDE3B lowers cytoplasmic cAMP (cyt‐cAMP) without affecting plasma membrane cAMP (pm‐cAMP). •Insulin decreases lipid droplet cAMP (LD‐cAMP) independent of PDE3B. •FGF1/PDE4D modestly reduces both cyt‐ and pm‐cAMP, while PDE4D alone can modulate LD‐cAMP. ISO stimulation: •Ins/PDE3B has minimal impact on cyt‐cAMP.
Johannes Krier, David Spähn, David Arturo Juarez Lopez, Judith L. Nono, Judith Seigner, Lisa Jacob, Siegfried Ussar, Robert Lukowski, Andreas L. Birkenfeld, Gencer Sancar   +9 more
wiley   +1 more source

Structure-Guided Optimization of PDE9A Inhibitors Tackling Neuroinflammation: A Combined Computational and In Vitro Study. [PDF]

ChemMedChem
Computational studies allowed the identification of a PDE9A inhibitor. A novel HPLC‐based assay was developed and optimized to measure enzyme function to assess PDE9A inhibition. Furthermore, in vitro tests supported the activity of the compound against neuroinflammation. Oxidative stress and inflammation are key players in central nervous system (CNS)
Ribaudo G, Landucci E, Anyanwu M, Mazzantini C, Giannangeli M, Rosa S, Mainolfi F, Calvani M, Memo M, Pellegrini-Giampietro DE, Gianoncelli A.   +10 more
europepmc   +2 more sources

An intracellular recombinant single‐chain variable antibody fragment as a new class of phosphodiesterase type 5 inhibitors

British Journal of Pharmacology, EarlyView.
Background and Purpose Cyclic guanosine monophosphate (cGMP) is a ubiquitous second messenger involved in human (patho‐)physiology. Phosphodiesterase 5 (PDE5) is a major cGMP hydrolyzing enzyme in many cell types including vascular smooth muscle cells (VSMCs). Several highly selective PDE5 inhibitors are in clinical use. However, there are currently no
Kürsat Kirkgöz, Sophie Sprenger, Olga Schweigert, Sanika Mohagaonkar, Matti Walsdorff, Tanja Zeller, Filip Berisha, Viacheslav O. Nikolaev, Sergei D. Rybalkin   +8 more
wiley   +1 more source

Transgenic Mice for Real Time Visualization of cGMP in Intact Adult Cardiomyocytes.

, 2014
cGMP is an important second messenger which regulates cardiac contractility and protects the heart from hypertrophy. However, due to the lack of real time imaging techniques, specific subcellular mechanisms and spatio-temporal dynamics of cGMP in adult ...
Götz, Konrad R., Nikolaev, Viacheslav O, Steinbrecher, Julia H, Götz, Konrad, Sprenger, Julia, Kuhn, Michaela, Nikolaev, Viacheslav O., Perera, Ruwan K., Steinbrecher, Julia H., Perera, Ruwan K, Balligand, Jean-Luc, Gorelik, Julia, Sprenger, Julia U., Lehnart, Stephan E, Lehnart, Stephan Elmar   +14 more
core   +1 more source

Differential segment‐specific signalling pathways for guanylate cyclase C‐activated anion secretion in murine ileocolon

British Journal of Pharmacology, EarlyView.
Abstract Background and Purpose Guanylate cyclase‐C (GC‐C) is the receptor for endogenous (uro)guanylin peptides, bacterial toxins and pharmacological analogues. Receptor activation leads to intestinal fluid loss, but also activates an antiproliferative pathway and is a promising target in colorectal cancer therapy.
Renjie Xiu, Johannes Reiner, Franz Hofmann, Azam Salari, Ursula Seidler   +4 more
wiley   +1 more source

The GAF Domain of the cGMP-Binding, cGMP-Specific Phosphodiesterase (PDE5) Is a Sensor and a Sink for cGMP

, 2008
We describe here a novel sensor for cGMP based on the GAF domain of the cGMP-binding, cGMP-specific phosphodiesterase 5 (PDE5) using bioluminescence resonance energy transfer (BRET).
Biswas, Kabir Hassan, Visweswariah, Sandhya S., Sopory, Shailaja, Visweswariah, Sandhya S   +3 more
core   +1 more source

Targeting hexokinase 2 to induce breast cancer cell senescence

British Journal of Pharmacology, EarlyView.
Background and Purpose Hexokinase 2 (HK2) is a key enzyme linked to high tumour cell proliferation. Its inhibitors such as 3‐bromopyruvic acid (3‐BP) induce cancer cell death, highlighting HK2 modulation as potential anti‐cancer treatment. However, standard chemotherapies often cause the emergence of senescent cancer cells, which goes along with cell ...
Helmut Bischof, Katarina Cisarova, Sandra Burgstaller, Mia Schwerer, Markus Absenger‐Novak, Philipp J. Jost, Roland Malli, Wolfgang F. Graier, Robert Lukowski   +8 more
wiley   +1 more source