The Journal of Physiology, EarlyView.Abstract figure legend Intricate cellular electrical coupling networks in the heart. Various cell types couple the central cardiomyocyte through gap junctional contacts, with the exception of neurons. Whether ephaptic coupling (EpC) occurs in homocellular or heterocellular contexts beyond cardiomyocyte–cardiomyocyte interactions remains unclear ...
Xiaobo Wu +2 more
wiley +1 more source
Long range channelrhodopsin-assisted circuit mapping of inferior colliculus neurons with blue and red-shifted channelrhodopsins [PDF]
Journal of Visualized Experiments, 2019 ABSTRACTWhen investigating neural circuits, a standard limitation of the in vitro patch clamp approach is that axons from multiple sources are often intermixed, making it difficult to isolate inputs from individual sources with electrical stimulation.
Goyer, David, Roberts, Michael T.
openaire +2 more sources
Sparsened neuronal activity in an optogenetically activated olfactory glomerulus [PDF]
, 2018 © The Author(s), 2018. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Scientific Reports 8 (2018): 14955, doi:10.1038/s41598-018-33021-w.Glomeruli are the functional units ...
Bozza, Thomas +6 more
core +2 more sources
Optimal neuromuscular performance requires motor neuron phosphagen kinases
The Journal of Physiology, EarlyView.Abstract figure legend Motor neuron (MN) terminals in fruit flies, mice and humans contain phosphagen kinases, a key enzyme in their energy storage and buffering system. Here we knocked down the primary phosphagen kinase in fruit fly larvae (arginine kinase 1 (ArgK1)) but were surprised to find that MN endurance was unaffected.
Karlis A. Justs +10 more
wiley +1 more source
Principles of Optogenetic Methods and Their Application to Cardiac Experimental Systems
Frontiers in Physiology, 2019 Optogenetic techniques permit studies of excitable tissue through genetically expressed light-gated microbial channels or pumps permitting transmembrane ion movement.
Emily A. Ferenczi +3 more
doaj +1 more source
Identifying specific prefrontal neurons that contribute to autism-associated abnormalities in physiology and social behavior. [PDF]
, 2017 Functional imaging and gene expression studies both implicate the medial prefrontal cortex (mPFC), particularly deep-layer projection neurons, as a potential locus for autism pathology.
Brumback, AC +9 more
core +1 more source
Caenorhabditis elegans as an in vivo model system for human inherited primary arrhythmia syndromes
The Journal of Physiology, EarlyView.Abstract figure legend Most genes involved in inherited primary arrhythmia syndromes (IPAS) are conserved in Caenorhabditis elegans, where genetic manipulation enables functional characterization of variants, identification of regulatory proteins, and in vivo drug testing.
Antoine Delinière +6 more
wiley +1 more source
Light and pH-induced Changes in Structure and Accessibility of Transmembrane Helix B and Its Immediate Environment in Channelrhodopsin-2 [PDF]
, 2016 A variant of the cation channel channelrhodopsin-2 from Chlamydomonas reinhardtii (CrChR2) was selectively labeled at position Cys-79 at the end of the first cytoplasmic loop and the beginning of transmembrane helix B with the fluorescent dye ...
Alexiev, Ulrike +7 more
core +1 more source
Light‐Based Molecular Tools to Precisely Monitor and Operate β‐Adrenoceptors
Medicinal Research Reviews, Volume 46, Issue 2, Page 445-474, March 2026.ABSTRACT β‐Adrenoceptors are important G protein–coupled receptors involved in cardiovascular, metabolic, and neurological regulation. To study their function with high precision, light‐based molecular tools have been developed offering precise spatiotemporal control.
Ignazzitto Maria Tindara +3 more
wiley +1 more source
Frontiers in Neural Circuits, 2013 Proper brain function relies on exquisite balance between excitation and inhibition, where inhibitory circuits play fundamental roles towards sculpting principle neuron output and information processing.
Longwen eHuang +3 more
doaj +1 more source