Results 11 to 20 of about 18,335 (296)
Investigating the feasibility of channelrhodopsin variants for nanoscale optogenetics [PDF]
Neurophotonics, 2019 . Optogenetics has revolutionized the study of circuit function in the brain, by allowing activation of specific ensembles of neurons by light. However, this technique has not yet been exploited extensively at the subcellular level.
Markus A. Stahlberg +5 more
semanticscholar +6 more sources
Optogenetic control of medaka behavior with channelrhodopsin
Development, Growth & Differentiation, 2023 Optogenetics enables the manipulation of neural activity with high spatiotemporal resolution in genetically defined neurons. The method is widely used in various model animals in the neuroscience and physiology fields.
Takahide Seki +2 more
semanticscholar +3 more sources
EMBO Molecular Medicine, 2016 Targeting the photosensitive ion channel channelrhodopsin‐2 (ChR2) to the retinal circuitry downstream of photoreceptors holds promise in treating vision loss caused by retinal degeneration. However, the high intensity of blue light necessary to activate
Abhishek Sengupta +12 more
doaj +2 more sources
Photoactivation of Channelrhodopsin [PDF]
Journal of Biological Chemistry, 2007 Channelrhodopsins (ChRs) are light-gated ion channels that control photomovement of microalgae. In optogenetics, ChRs are widely applied for light-triggering action potentials in cells, tissues, and living animals, yet the spectral properties and photocycle of ChR remain obscure.
Oliver P. Ernst +5 more
openalex +5 more sources
Unifying photocycle model for light adaptation and temporal evolution of cation conductance in channelrhodopsin-2 [PDF]
Proceedings of the National Academy of Sciences of the United States of America, 2019 Significance Understanding the mechanisms of photoactivated biological processes facilitates the development of new molecular tools, engineered for specific optogenetic applications, allowing the control of neuronal activity with light.
Jens Kühne +9 more
openalex +2 more sources
Ion Channel Properties of a Cation Channelrhodopsin, Gt_CCR4
Applied Sciences, 2019 We previously reported a cation channelrhodopsin, Gt_CCR4, which is one of the 44 types of microbial rhodopsins from a cryptophyte flagellate, Guillardia theta.
Shunta Shigemura +3 more
doaj +2 more sources
Structural Model of Channelrhodopsin [PDF]
Journal of Biological Chemistry, 2012 Channelrhodopsins (ChRs) are light-gated cation channels that mediate ion transport across membranes in microalgae (vectorial catalysis). ChRs are now widely used for the analysis of neural networks in tissues and living animals with light (optogenetics).
Hiroshi Watanabe +7 more
openalex +6 more sources
Use of Channelrhodopsin for Activation of CNS Neurons [PDF]
Current Protocols in Neuroscience, 2012 AbstractOptogenetics—the use of optically activated proteins to control cell function—allows for control of neurons with an unprecedented degree of spatial, temporal, and neurochemical precision. Three protocols are presented in this unit describing the use of channelrhodopsin‐2 (ChR2), a light‐activated cation channel.
Jonathan P. Britt +2 more
openalex +4 more sources
Intramolecular Proton Transfer in Channelrhodopsins [PDF]
Biophysical Journal, 2013 Channelrhodopsins serve as photoreceptors that control the motility behavior of green flagellate algae and act as light-gated ion channels when heterologously expressed in animal cells. Here, we report direct measurements of proton transfer from the retinylidene Schiff base in several channelrhodopsin variants expressed in HEK293 cells.
Oleg A. Sineshchekov +4 more
openalex +5 more sources
The form and function of channelrhodopsin [PDF]
Science, 2017 From biophysics to neuroscience tools The channelrhodopsins and their distinctive light-activated ion channels have emerged as major tools in modern biological research. Deisseroth and Hegemann review the structural and functional properties of these protein photoreceptors.
K. Deisseroth, P. Hegemann
semanticscholar +4 more sources