Hemodynamic responses evoked by neuronal stimulation via channelrhodopsin-2 can be independent of intracortical glutamatergic synaptic transmission. [PDF]
PLoS ONE, 2012 Maintenance of neuronal function depends on the delivery of oxygen and glucose through changes in blood flow that are linked to the level of ongoing neuronal and glial activity, yet the underlying mechanisms remain unclear.
Nadia A Scott, Timothy H Murphy
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Optogenetics in Alzheimer’s Disease: Focus on Astrocytes
Antioxidants, 2023 Alzheimer’s disease (AD) is the most common form of dementia, resulting in disability and mortality. The global incidence of AD is consistently surging.
Elena Mitroshina +2 more
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Frontiers in Molecular Biosciences, 2022 The translocon-unassisted folding process of transmembrane domains of the microbial rhodopsins sensory rhodopsin I (HsSRI) and II (HsSRII), channelrhodopsin II (CrChR2), and bacteriorhodopsin (HsBR) during cell-free expression has been investigated by ...
Kenichi Ataka +5 more
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Cryo-EM structures of the channelrhodopsin ChRmine in lipid nanodiscs
Nature Communications, 2021 Microbial channelrhodopsins are light-gated ion channels widely used for optogenetic manipulation of neuronal activity. ChRmine is a bacteriorhodopsin-like cation channelrhodopsin (BCCR) more closely related to ion pump rhodopsins than other ...
K. Tucker +3 more
semanticscholar +1 more source
Non-viral delivery and optimized optogenetic stimulation of retinal ganglion cells led to behavioral restoration of vision [PDF]
, 2012 Stimulation of retinal neurons using optogenetics via use of chanelrhodopsin-2 (ChR2) has opened up a new direction for restoration of vision for treatment of retinitis pigmentosa (RP).
Edward Wong +5 more
core +2 more sources
An engineered channelrhodopsin optimized for axon terminal activation and circuit mapping
Communications Biology, 2021 Optogenetic tools such as channelrhodopsin-2 (ChR2) enable the manipulation and mapping of neural circuits. However, ChR2 variants selectively transported down a neuron’s long-range axonal projections for precise presynaptic activation remain lacking. As
S. Hamada +6 more
semanticscholar +1 more source
Fast targeted gene transfection and optogenetic modification of single neurons using femtosecond laser irradiation [PDF]
, 2013 This work is supported by the UK Engineering Physical Sciences Research Council (EPSRC).A prevailing problem in neuroscience is the fast and targeted delivery of DNA into selected neurons.
Antkowiak, Maciej +5 more
core +1 more source
Frontiers in Physiology, 2021 Optogenetic defibrillation of hearts expressing light-sensitive cation channels (e.g., ChR2) has been proposed as an alternative to conventional electrotherapy.
Alexander R Ochs +3 more
semanticscholar +1 more source
Functional cardiac imaging by random access microscopy
Frontiers in Physiology, 2014 Advances in the development of voltage sensitive dyes and Ca2+ sensors in combination with innovative microscopy techniques allowed researchers to perform functional measurements with an unprecedented spatial and temporal resolution.
Claudia eCrocini +7 more
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Biophysical properties of optogenetic tools and their application for vision restoration approaches.
Frontiers in Systems Neuroscience, 2016 Optogenetics is the use of genetically encoded light-activated proteins to manipulate cells in a minimally invasive way using light. The most prominent example is channelrhodopsin2 (ChR2), which allows the activation of electrically excitable cells via ...
Simon Darius Klapper +3 more
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