Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Antioxidant and Anticancer Potentials of Apple Peel and Fruit Extracts: A Combined Docking and Chemical Composition Study. [PDF]
Hançer A +5 more
europepmc +1 more source
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Chemical Composition, Physicochemical Properties, and Antimicrobial Activity of Stingless Bee Geopropolis from the Baturité Massif, Northeastern Brazil. [PDF]
Teixeira JVL +12 more
europepmc +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Chemical composition and bioinsecticidal activity of bioinputs produced by Saccharopolyspora spinosa. [PDF]
Costa LFR +12 more
europepmc +1 more source
Comparative evaluation of irrigation techniques on dentin nanohardness, flexural strength, and chemical composition: an in vitro study. [PDF]
Ahmed RA, AbuMostafa A.
europepmc +1 more source
Effect of Distillation Time on the Yield and Chemical Composition of Leaf Essential Oil from <i>Abies koreana</i>. [PDF]
Park C, Kim N, Jang SK, Park MJ.
europepmc +1 more source

