Results 181 to 190 of about 5,463,250 (252)

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

From Waste Paper to Regenerated Nanocellulose: Purification Strategy Controls the Residual Chemical Fingerprint. [PDF]

Polymers (Basel)
Raclavská H, Švédová B, Šafář M, Kucbel M, Růžičková J, Matýsek D, Slamová K, Kantor P.   +7 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Dynamics of Rare Earth Element Uptake and Partitioning in Two Soybean Cultivars Under Background Soil Conditions. [PDF]

Plants (Basel)
Chen Y, Lu J, Zhao X, Lu P, Cui J, Zhang K, Qu J, Hou Y.   +7 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Comparative study of the impacts of three common fractionation methods on the physicochemical features of lignin. [PDF]

RSC Adv
Wang Y, Seabright K, Zhu H, Zhou H, Harper DP, Li M.   +5 more
europepmc   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Oxidative Bagasse Fractionation for Its Efficient Utilization Based on a Lignin-First Approach. [PDF]

ChemSusChem
Kong J, Zhang X, Hu H, Lian L, Liu S, Li X.   +5 more
europepmc   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source

Antioxidant, Antibacterial, and Antivirulence Activities of a Bioactive Fraction from <i>Lycopus lucidus</i> Against <i>Porphyromonas gingivalis</i>. [PDF]

Antioxidants (Basel)
Park JM, Park S, Hwang DY, Lee H, Park J.   +4 more
europepmc   +1 more source