Results 191 to 200 of about 109,469 (312)

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Isotopic Fractionation and Masking Effects during Biotransformation of Chlorinated Ethanes. [PDF]

Environ Sci Technol
Phillips E, De Vera J, Kümmel S, Bulka O, Chen W, Edwards E, Nijenhuis I, Gehre M, Sherwood Lollar B.   +8 more
europepmc   +1 more source

Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids

FEBS Open Bio, EarlyView.
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão, Diogo M. Engrácia, Graça Soveral, Filipa Mendes   +3 more
wiley   +1 more source

Uncovering Bioactive Compounds in Propolis Extracts Prior to Isolation Through NMR Chemometric Analysis. [PDF]

Molecules
Stavropoulou MI, Cheilari A, Graikou K, Chinou I, Aligiannis N.   +4 more
europepmc   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

As above, not so below: Ion fractionation in planetary analog ices. [PDF]

Sci Adv
Buffo JJ, Fox-Powell MG, Murdza A, Tomlinson TC, Schultz A, Barton T, Gurd C, McEwen A, Wolfenbarger NS, Chivers CJ, Schmidt BE, Meyer CR.   +11 more
europepmc   +1 more source

Small RNA pathways in mammalian oocytes

FEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

Separation of saponins from quinoa bran by a novel single-column three-stage foam fractionation technology. [PDF]

Food Chem X
Jia L, Liu W, Ge X, Miao X, Zhang K.
europepmc   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source

Climatic imprint on interfacially controlled platinum-palladium resources. [PDF]

PNAS Nexus
Wright EG, Wang I, Fang Y, Flynn ED, Catalano JG.   +4 more
europepmc   +1 more source