Results 121 to 130 of about 7,468,932 (307)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins
FEBS Open Bio, EarlyView.Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley +1 more source
Acreage Abandonment, Moral Hazard and Crop Insurance [PDF]
Empirical evidence for the existence of moral hazard in the U.S. crop insurance program has been inconclusive. Here we use a nested-dynamic programming framework to estimate an intra-seasonal dynamic model that explicitly incorporates a farmer's crop ...
Chen, Shu-Ling
core
Orapuh JournalIntroduction In Kinshasa, kaolin consumption is a deeply rooted cultural practice, particularly among women. However, it represents a major exposure pathway for chemical contaminants, while the toxicological risks associated with trace metal elements
Maria The First Ngangula +7 more
doaj +1 more source
FEBS Open Bio, EarlyView.Repair of neuronal DNA damage in Alzheimer's disease by KCL‐286. (A) Amyloid‐β oligomers and plaques impair neuronal DNA repair pathways, leading to DNA double‐strand breaks and glial activation. (B) KCL‐286 activates RARβ/RXR signalling via retinoic acid response elements (RAREs), associated with increased BRCA1 expression, enhanced DNA repair and ...
Natasha Hill +6 more
wiley +1 more source
FEBS Open Bio, EarlyView.Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas +6 more
wiley +1 more source
AgriculturePhosphogypsum (PG) is a bulk industrial solid waste generated during wet-process phosphoric acid production. Its agricultural use may improve specific soil properties but may also increase the risk of agricultural non-point-source pollution.
Qi Liu +5 more
doaj +1 more source
FEBS Open Bio, EarlyView.Lactoferrin promotes wound healing by activating MAPK, PI3K/Akt, and extracellular matrix pathways, enhancing fibroblast remodeling, proliferation, and re‐epithelialization. These mechanisms highlight its therapeutic potential in inflammation control, tissue repair, and remodeling.
Morgana Lüdtke Azevedo +4 more
wiley +1 more source
Integration of a Reactive Chemicals Program into the Process Hazard Analysis Process at The Dow Chemical Company [PDF]
, 2001 PresentationDow's Reactive Chemicals Program has been in place since 1967 and has been utilized globally by Dow to reduce the hazards associated with reactive chemical scenarios.
Overton, Tim, Phillips, Gary
core
Problems of Gathering, Treatment and Transportation of Oil and Oil Products, 2021 A.A. Aleksandrov +2 more
openaire +1 more source