Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Green spectrophotometric methods for the determination of metformin-curcumin combination: ICH validation and preliminary bioanalytical applications. [PDF]
El-Masry AA +2 more
europepmc +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
Oral Cavity Antibacterial Discovery Pipeline Driven by Advanced Analytical Techniques. [PDF]
Aresta AM +4 more
europepmc +1 more source
Research is strongest when conducted alongside patients, not just about them. Patient research organizations help integrate patient perspectives into research priorities, study design, and scientific meetings, leading to meaningful patient outcomes and development of relevant therapies.
Jenica H. Kakadia +9 more
wiley +1 more source
Multi-Metric Assessment of Liquid Chromatography-Mass Spectrometry Methods for Mycotoxin Determination in Human Urine: Advancing Sustainable, Practical, and Innovative Analytical Practices. [PDF]
Alqarni AM +8 more
europepmc +1 more source
Dynamic photobiology: A collection of experimental protocols for time-resolved structural studies on light-activated proteins. [PDF]
Kapetanaki SM, Royant A, Yorke BA.
europepmc +1 more source
Simple and cost-effective UV spectrophotometric platforms integrating advanced green and blue metrics for concurrent analysis of dapagliflozin and vildagliptin in diabetes therapy. [PDF]
Darweish E +3 more
europepmc +1 more source
Eco-friendly spectrofluorimetric determination of fenbendazole in pharmaceutical, plasma, and milk samples using erythrosine B as a fluorescent probe. [PDF]
Mohamad AAA +3 more
europepmc +1 more source
An Eco-Friendly N-Doped Carbon Quantum Dot-Based Fluorimetric Assay for Sensitive Betahistine Quantitation in Pharmaceuticals and Biological Fluids. [PDF]
Kamel MA +4 more
europepmc +1 more source

