Results 41 to 50 of about 44,236 (261)

Attenuation of a Field Strain of Infectious Laryngotracheitis Virus in Primary Chicken Culture Cells and Adaptation to Secondary Chicken Embryo Fibroblasts

Poultry, 2023
The establishment of commercial infectious laryngotracheitis virus (ILTV) live-modified vaccines has relied on serial passaging in chicken embryo (CEO) and tissue culture (TCO) for attenuation.
Victor A. Palomino-Tapia, Guillermo Zavala, Sunny Cheng, Maricarmen Garcia   +3 more
doaj   +1 more source

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Longitudinal volumetric analysis of in ovo compartments in chicken eggs using ultra-high-field magnetic resonance imaging

Frontiers in Veterinary Science
IntroductionThe chicken egg, with its in ovo compartments, is a widely used and popular animal model in experimental studies. This study aimed to quantify the volumes of the yolk/yolk sac, amniotic fluid, and chicken embryo in ovo using non-invasive ...
Felix Streckenbach, Felix Streckenbach, Hanna Schön, Hanna Schön, Julia König, Marcus Frank, Marcus Frank, Inga Langner, Oliver Stachs, Oliver Stachs, Anika Jonitz-Heincke, Anika Jonitz-Heincke, Sönke Langner, Tobias Lindner, Tobias Lindner, Jana Schätzel   +15 more
doaj   +1 more source

Research on the appropriate way to transfer exogenous substances into chicken embryos

Journal of Integrative Agriculture, 2017
: In biological research, chicken embryos are a classic experimental model for the exploration of the embryonic development and cell differentiation. Transferring exogenous substances into chicken embryos for producing medical antibodies has been widely ...
Yi-lin WANG, Kai JIN, Na-na HE, Shao-ze CHENG, Qi-sheng ZUO, Dong LI, Ying-jie WANG, Fei WANG, Yan-qing JI, Zhen-yu LU, Chen ZHANG, Man WANG, Rui-feng ZHAO, Xin-jian YU, Ya-ni ZHANG, Wen-ming ZHAO, Bi-chun LI   +16 more
doaj   +1 more source

An Aberrant Parthenogenetic Chicken Embryo

Poultry Science, 1972
Abstract A virgin Dark Cornish hen laid an egg which began to develop parthenogenetically. The embryo thus formed was harvested for chromosomal analysis after twenty-two hours of incubation. A unique translocation between one member of each of the largest and third largest pairs of chromosomes was found in all thirty-seven metaphase cells observed ...
openaire   +2 more sources

Acute caffeine treatment protects the developing retina from ischemia‐induced cell death

FEBS Open Bio, EarlyView.
Caffeine reduces cell death in the developing retina under ischemia (OGD). This effect does not involve BDNF upregulation or antioxidant pathways (NRF2/VEGF). Neuroprotection occurs mainly through adenosine A2A receptor antagonism, decreasing glutamate release and excitotoxicity, highlighting caffeine's potential as an acute neuroprotective agent in ...
Amanda Alves Nascimento, Danniel Pereira‐Figueiredo, Gabriella Grossi de Lima Dias, Gabriel Ferreira dos Santos, Mariana Rodrigues Pereira, Rafael Brito, Karin C Calaza   +6 more
wiley   +1 more source

Chicken Embryo Malpositions and Deformities

EDIS, 2003
The objective of this study was to determine the relative incidences of malpositions and deformities, and their economic impacts. Major factors affecting their occurrence will be explained.
Gary D. Butcher, Amir H. Nilipour
doaj   +5 more sources

IDENTIFICATION OF SUITABLE CULTURE SYSTEM FOR FIELD FOWL ADENOVIRUS ISOLATION [PDF]

Exploratory Animal and Medical Research
The Present study was aimed to find out the suitable culture system for field fowl adenovirus isolation. Liver tissues were collected from fowl aviadenovirus suspected commercial broiler flocks (n=100 f locks) showing inclusion body hepatitis (IBH ...
S. Chitradevi
doaj   +1 more source

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

FEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim, Marco Corazzari, Iman Osman, Jane Armstrong, Noel Carter   +4 more
wiley   +1 more source