Results 1 to 10 of about 32,552 (229)
The cold case of state transition 7 (stt7) mutants of Chlamydomonas reinhardtii, solved by whole-genome sequencing. [PDF]
New PhytolLong‐read whole‐genome sequencing revealed extensive chromosomal rearrangements and aneuploidy in a widely used Chlamydomonas reinhardtii stt7‐1 mutant. The STT7 gene (shown in red) was found to be split between two rearranged chromosomes, providing a molecular explanation for the long‐standing failure of this mutant in genetic crosses.
Bujaldon S +3 more
europepmc +2 more sources
Estimation of chloroplast macromolecular complex copy numbers and subunit stoichiometries during the Chlamydomonas reinhardtii cell cycle [PDF]
Plant JSUMMARY An unbiased, quantitative view of biomolecules in a living cell is a prerequisite for accurate modeling approaches and informs our understanding of cellular metabolism at scale. In this work, we used the total protein approach (TPA), in which the total protein mass of a given proteomics sample is used as a calibrator for absolute protein ...
Schmollinger S +6 more
europepmc +2 more sources
Expression of recombinant Cap antigen of porcine circovirus type 2 in the chloroplast of Chlamydomonas reinhardtii [PDF]
Frontiers in Plant ScienceThe capsid (Cap) protein encoded by the ORF2 gene of porcine circovirus type 2 is the major immunogen for the development of vaccines and can effectively reduce the incidence of porcine circovirus-associated diseases.
Yiran Song +5 more
doaj +2 more sources
Selenoprotein: Potential Player in Redox Regulation in Chlamydomonas reinhardtii
Antioxidants, 2022 Selenium (Se) is an essential micro-element for many organisms, including Chlamydomonas reinhardtii, and is required in trace amounts. It is obtained from the 21st amino acid selenocysteine (Sec, U), genetically encoded by the UGA codon.
Sandip A. Ghuge +2 more
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Functional characterization of the Chlamydomonas reinhardtii ERG3 ortholog, a gene involved in the biosynthesis of ergosterol. [PDF]
PLoS ONE, 2010 The predominant sterol in the membranes of the alga Chlamydomonas reinhardtii is ergosterol, which is commonly found in the membranes of fungi, but is rarely found in higher plants.
Kristy M Brumfield +4 more
doaj +1 more source
To Divide or Not to Divide? How Deuterium Affects Growth and Division of Chlamydomonas reinhardtii
Biomolecules, 2021 Extensive in vivo replacement of hydrogen by deuterium, a stable isotope of hydrogen, induces a distinct stress response, reduces cell growth and impairs cell division in various organisms.
Veronika Kselíková +2 more
doaj +1 more source
MethodsX, 2020 A recently reported protocol demonstrates efficient CRISPR/Cas9 gene editing of Chlamydomonas reinhardtii [1]. The published protocol demonstrates transformation and editing of a wall-less strain of C.
Rudolph V. Park +2 more
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Biotechnology for Biofuels and Bioproducts, 2022 Background Astaxanthin is a highly valuable ketocarotenoid with strong antioxidative activity and is natively accumulated upon environmental stress exposure in selected microorganisms. Green microalgae are photosynthetic, unicellular organisms cultivated
Stefano Cazzaniga +3 more
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A two-phase protocol for ambient hydrogen production using Chlamydomonas reinhardtii
STAR Protocols, 2022 Summary: H2 production from green-microalgae, for energy purposes, is the ultimate goal of large-scale production. Here, we present a two-phase protocol for hydrogen production assay under ambient conditions using Chlamydomonas reinhardtii, which ...
Tamar Elman, Iftach Yacoby
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Bio-Protocol, 2013 This protocol describes the affinity purification of the chloroplast ClpP complex from Chlamydomonas reinhardtii. To this purpose, we have created a Chlamydomonas reinhardtii strain in which the chloroplast encoded ClpP1 subunit of the ClpP complex is ...
Benoit Derrien, Olivier Vallon
doaj +1 more source