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isolation of Wheat Chloroplasts and Chloroplast DNA

2003
Chloroplasts in common with other plastids and mitochondria have their own nucleic material, in the form of multiple copies of a circular DNA molecule, normally of 120-160 kbp.
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Nicotiana chloroplast genome III. Chloroplast DNA evolution

Theoretical and Applied Genetics, 1982
Nicotiana chloroplast genomes exhibit a high degree of diversity and a general similarity as revealed by restriction enzyme analysis. This property can be measured accurately by restriction enzymes which generate over 20 fragments. However, the restriction enzymes which generate a small number (about 10) of fragments are extremely useful not only in ...
S D, Kung, Y S, Zhu, G F, Shen
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Chloroplast biosystematics: Chloroplast DNA as a molecular probe

Biosystems, 1985
The classification of plants has traditionally been dependent upon the comparative analysis of morphological and biochemical data. In this paper the use of molecular probe analysis of chloroplast DNA (ctDNA) is used to expand the data base used in taxonomic studies.
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Hybridization of bean chloroplast transfer RNAs to chloroplast DNA

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1976
Bean (Phaseolus vulgaris) chloroplast tRNAsLeu and tRNAsPhe hybridize to chloroplast DNA, whereas the corresponding cytoplasmic tRNA species do not, suggesting that chloroplast transfer RNAs are coded for by chloroplast DNA. The hybridization of the three chloroplast tRNAsLeu or of the two tRNAsPhe isoacceptors is not additive, and the isoacceptors ...
A, Steinmetz, J H, Well
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The Structure of Chloroplast DNA

Annual Review of Plant Physiology, 1979
INTRODUCTION . PHYSICAL STUDIES ON THE STRUCTURE OF ctDNA . Kinetic Comple;city of ctDNA . Circularity of ctDNA .
J R Bedbrook, R Kolodner
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DNA Synthesis in Chloroplasts

Journal of Experimental Botany, 1989
The influence of two DNA gyrase inhibitors, nalidixic acid and novobiocin on DNA synthesis in isolated pea chloroplasts was examined by autoradiography and ...
W. RONALD MILLS   +3 more
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Chloroplast ribosomal RNA genes in the chloroplast DNA of Euglena gracilis

Journal of Molecular Biology, 1973
Euglena chloroplast DNA has a buoyant density in CsCI of 1.686. Shearing this DNA produces a satellite band at density 1.700. The satellite, easily lost during preparative CsCI gradient centrifugation of chloroplast DNA, contains the genes for chloroplast ribosomal RNA. Pure Euglena chloroplast DNA is shown to contain one set of ribosomal RNA genes for
J R, Rawson, R, Haselkorn
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Isolation of Maize Chloroplasts and Chloroplast DNA

1994
This procedure is for the isolation of chloroplasts and highly purified chloroplast DNA from 1 kg of maize seedling leaves. Because starch reduces the yield of intact chloroplasts, light-grown seedlings are placed into darkness for 1–2 days prior to DNA isolation. All manipulations should be performed at 4°C, unless otherwise noted.
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The characteristics of spinach chloroplast DNA polymerase

Archives of Biochemistry and Biophysics, 1969
Purified chloroplast preparations from spinach incorporate [3H]-dTTP1 into an acid-insoluble product. The incorporation reaction is highly dependent on all four deoxynucleoside triphosphates, Mg2+ and a sulphydryl reagent, and is further stimulated by KCl.
D, Spencer, P R, Whitfeld
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Chloroplast DNA from three archegoniates

Planta, 1980
1. DNA from female and male Sphaerocarpos donnellii (liverwort) plants exhibits at least two species with buoyant densities of 1.703 (main band) and 1.691 (satellite) g cm(-3) in CsCl equilibrium gradients. At least part, if not all, of the satellite DNA is localized in plastids.
R G, Herrmann, H K, Palta, K V, Kowallik
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