Results 301 to 310 of about 663,360 (333)

Dynamic model for CHO cell engineering

Journal of Biotechnology, 2012
Industrial CHO cell fed-batch processes have progressed significantly over the past decade, with recombinant protein titer consistently reaching the gram per liter level. Such improvements have largely resulted from separate advances in process and cell line development.
Ryan P, Nolan, Kyongbum, Lee
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Synthetic promoters for CHO cell engineering

Biotechnology and Bioengineering, 2014
ABSTRACTWe describe for the first time the creation of a library of 140 synthetic promoters specifically designed to regulate the expression of recombinant genes in CHO cells. Initially, 10 common viral promoter sequences known to be active in CHO cells were analyzed using bioinformatic sequence analysis programs to determine the identity and relative ...
Adam J, Brown, Bernie, Sweeney, David O, Mainwaring, David C, James   +3 more
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CRISPR‐interceded CHO cell line development approaches

Biotechnology and Bioengineering, 2023
AbstractFor industrial production of recombinant protein biopharmaceuticals, Chinese hamster ovary (CHO) cells represent the most widely adopted host cell system, owing to their capacity to produce high‐quality biologics with human‐like posttranslational modifications.
Shahin Amiri, Setare Adibzadeh, Samaneh Ghanbari, Behnaz Rahmani, Mohammad H. Kheirandish, Aref Farokhi‐Fard, Mansoureh S. Dastjerdeh, Fatemeh Davami   +7 more
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Selenalysine utilization by CHO cells.

Physiological chemistry and physics and medical NMR, 1987
CHO cells allowed to grow in a medium containing selenalysine can utilize it for protein synthesis. Selenalysine is incorporated into cell proteins in substitution of lysine: a maximum of 5% of protein lysine can be substituted. Protein lysine substitution by selenalysine can be correlated to the reduced viability of cells grown in its presence.
C. D. Marco, CINI, Chiara, BLARZINO, Carla, V. Busiello, M. D. Girolamo   +4 more
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Genotoxic effects of ketamine on CHO cells

Archives of Toxicology, 1986
Ketamine, a non-barbiturate anaesthetic agent, was studied for its genotoxic potential using the SCE assay. It was genotoxic in the in vitro system at concentrations comparable to the plasma levels achieved during steady state anaesthesia. It had no effects on cellular kinetics in CHO cells.
S G, Adhvaryu, A H, Trivedi, K H, Jani, R C, Vyas, B J, Dave   +4 more
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cAMP-regulated chloride currents in CHO cells

Biochemical and Biophysical Research Communications, 1992
We examined whether elevations in cAMP levels increase membrane chloride permeability in native CHO cells by measuring whole cell chloride currents and efflux of 125I and 36Cl. With 20 microM forskolin, no significant effect was seen on whole cell currents.
A W, Mangel, S, Basavappa, J R, Raymond, J G, Fitz   +3 more
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Effects of selenalysine on CHO cells.

Microbiologica, 1986
Selenalysine, the lysine isolog with the 4-methylene group substituted by a Selenium atom, inhibits growth rate and plating efficiency of Chinese Hamster Ovary (CHO) cells. It does not affect DNA and RNA synthesis, but inhibits protein synthesis. Cells grown in the presence of selenalysine show a reduced viability and an increased cell volume.
CINI, Chiara, COCCIA, Raffaella, C. Foppoli, M. Canini, M. D. Girolamo   +4 more
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CONTINUOUS SUSPENSION CHO CELL CULTURE

1994
ABSTRACT A recombinant DNA CHO cell line which produces t-PA was cultivated continuously in suspension (D = 0.5 h−1). The cultivation consisted of four phases with different ammonium chloride concentrations (0,2.5,5 and 7.5 mM) in the feed medium, causing reactor ammonium levels up to 8 mM.
Henrik Albahn Hansen, Claus Emborg
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Activation of transglutaminase during cell cycle in CHO cells

Journal of Cellular Physiology, 1982
AbstractTransglutaminase (TGase) activity was measured during cell cycle progression in Chinese hamster ovary (CHO) cells synchronized by release of quiescent cultures and in CHO cells synchronized by mitotic shake off. In cells released from quiescent cultures, a greater than 2‐fold increase in TGase activity occurred within 3 h of stimulation ...
K F, Scott, D H, Russell
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Genotoxicity of goniothalamin in CHO cell line

Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 2004
Goniothalamin (GTN) is a styrylpyrrone derivative from Goniothalamus umbrosus and other Annonaceae species. It has been shown to have anti-cancer and apoptosis-inducing properties against various human tumour and animal cell lines. The compound has also been shown to be active in vivo against DMBA-induced rat mammary tumours and was reported as an anti-
Nasir, Umar-Tsafe, Mohamed Saifulaman, Mohamed-Said, Rozita, Rosli, Laily Bin, Din, Leslie C, Lai   +4 more
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