Results 361 to 370 of about 11,449,307 (399)

Genotoxic effects of ketamine on CHO cells

Archives of Toxicology, 1986
Ketamine, a non-barbiturate anaesthetic agent, was studied for its genotoxic potential using the SCE assay. It was genotoxic in the in vitro system at concentrations comparable to the plasma levels achieved during steady state anaesthesia. It had no effects on cellular kinetics in CHO cells.
A. H. Trivedi, Bhavana J. Dave, S. G. Adhvaryu, K. H. Jani, R. C. Vyas   +4 more
openaire   +3 more sources

Misonidazole-glutathione conjugates in CHO cells

International Journal of Radiation Oncology*Biology*Physics, 1984
Misonidazole, after reduction to the hydroxylamine derivative, reacts with glutathione (GSH) under physiological conditions. The reaction product has been identified as a mixture of two isomeric conjugates. When water soluble extracts of CHO cells exposed to misonidazole under hypoxic conditions are subjected to HPLC analysis, misonidazole derivatives,
G. F. Whitmore, A.J. Varghese
openaire   +3 more sources

Dynamic model for CHO cell engineering

Journal of Biotechnology, 2012
Industrial CHO cell fed-batch processes have progressed significantly over the past decade, with recombinant protein titer consistently reaching the gram per liter level. Such improvements have largely resulted from separate advances in process and cell line development.
Kyongbum Lee, Ryan P. Nolan
openaire   +3 more sources

Synthetic promoters for CHO cell engineering

Biotechnology and Bioengineering, 2014
ABSTRACTWe describe for the first time the creation of a library of 140 synthetic promoters specifically designed to regulate the expression of recombinant genes in CHO cells. Initially, 10 common viral promoter sequences known to be active in CHO cells were analyzed using bioinformatic sequence analysis programs to determine the identity and relative ...
Bernie Sweeney, David O. Mainwaring, David C. James, Adam J. Brown   +3 more
openaire   +3 more sources

Engineering Antibodies on the Surface of CHO Cells

2019
While antibody libraries are traditionally screened in phage, bacterial, or yeast display formats, they are produced in large scale for pharmaceutical and commercial use in mammalian cell lines. The simpler organisms used for screening have significantly different folding and glycosylation machinery than mammalian cells; consequently, clones resulting ...
Kevin C. Le, Annalee W. Nguyen, Jennifer A. Maynard   +2 more
openaire   +2 more sources

Genotoxicity of goniothalamin in CHO cell line

Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 2004
Goniothalamin (GTN) is a styrylpyrrone derivative from Goniothalamus umbrosus and other Annonaceae species. It has been shown to have anti-cancer and apoptosis-inducing properties against various human tumour and animal cell lines. The compound has also been shown to be active in vivo against DMBA-induced rat mammary tumours and was reported as an anti-
Leslie C Lai, Laily B. Din, Rozita Rosli, Nasir Umar-Tsafe, Mohamed Saifulaman Mohamed-Said   +4 more
openaire   +3 more sources

Targeted Gene Deletion Using DNA-Free RNA-Guided Cas9 Nuclease Accelerates Adaptation of CHO Cells to Suspension Culture.

ACS Synthetic Biology, 2016
Chinese hamster ovary (CHO) cells are the preferred host for the production of a wide array of biopharmaceuticals. Thus, efficient and rational CHO cell line engineering methods have been in high demand to improve quality and productivity.
Namil Lee, Jongoh Shin, Jin Hyoung Park, G. Lee, Suhyung Cho, Byung-Kwan Cho   +5 more
semanticscholar   +1 more source

Effects of selenalysine on CHO cells.

Microbiologica, 1986
Selenalysine, the lysine isolog with the 4-methylene group substituted by a Selenium atom, inhibits growth rate and plating efficiency of Chinese Hamster Ovary (CHO) cells. It does not affect DNA and RNA synthesis, but inhibits protein synthesis. Cells grown in the presence of selenalysine show a reduced viability and an increased cell volume.
CINI, Chiara, COCCIA, Raffaella, C. Foppoli, M. Canini, M. D. Girolamo   +4 more
openaire   +2 more sources

Selenalysine utilization by CHO cells.

Physiological chemistry and physics and medical NMR, 1987
CHO cells allowed to grow in a medium containing selenalysine can utilize it for protein synthesis. Selenalysine is incorporated into cell proteins in substitution of lysine: a maximum of 5% of protein lysine can be substituted. Protein lysine substitution by selenalysine can be correlated to the reduced viability of cells grown in its presence.
C. D. Marco, CINI, Chiara, BLARZINO, Carla, V. Busiello, M. D. Girolamo   +4 more
openaire   +2 more sources

Activation of transglutaminase during cell cycle in CHO cells

Journal of Cellular Physiology, 1982
AbstractTransglutaminase (TGase) activity was measured during cell cycle progression in Chinese hamster ovary (CHO) cells synchronized by release of quiescent cultures and in CHO cells synchronized by mitotic shake off. In cells released from quiescent cultures, a greater than 2‐fold increase in TGase activity occurred within 3 h of stimulation ...
Karen F. Frasier Scott, Diane Haddock Russell   +1 more
openaire   +3 more sources