Results 371 to 380 of about 11,449,307 (399)

An in-silico study of the regulation of CHO cells glycolysis

Journal of Theoretical Biology, 2014
In this work, a kinetic-metabolic model previously developed for CHO cells is used to study glycolysis regulation. The model is assessed for its biological relevance by analyzing its ability to simulate metabolic events induced following a hypoxic perturbation.
Olivier Henry, Mario Jolicoeur, Atefeh Ghorbaniaghdam   +2 more
openaire   +4 more sources

NF-κB, CRE and YY1 elements are key functional regulators of CMV promoter-driven transient gene expression in CHO cells.

Biotechnology Journal, 2015
Transient gene expression (TGE) in CHO cells is utilized to produce material for use in early stage drug development. These systems typically utilize the cytomegalovirus (CMV) promoter to drive recombinant gene transcription.
Adam J. Brown, Bernie Sweeney, David O. Mainwaring, D. James   +3 more
semanticscholar   +1 more source

Disruption of cytoskeleton by methylmercury in cultured CHO cells

Toxicology in Vitro, 1992
The effect of methylmercury (MM) on three main cytoskeletal components [i.e. microtubules (MT), microfilaments (MF) and intermediate filaments (IF)] and on specific biochemical parameters (i.e. glutathione transferase (GST), glutathione reductase (RED), glutathione peroxidase (GSH-Px), glyoxalase 1 (GLY 1) and total -SH groups (TSH) of the cytosolic ...
Vignani, R., Milanesi, C., Di Simplicio, P.   +2 more
openaire   +4 more sources

A CHO cell line resistant to deoxycholic acid

Cancer Letters, 1987
Spontaneous mutants resistant to deoxycholic acid (DCA) have been selected from a CHO cell line AuxBl. One of the colonies or 'lines' selected was subsequently mutagenized by ethylmethanesulfonate (250 micrograms/ml) and a more resistant cell, named alpha 3, has been selected. When AuxBl and alpha 3 were exposed to graded concentrations of DCA for 1 h,
CADERNI, GIOVANNA, Stuart E, Anderson AM, Bruce WR   +3 more
openaire   +4 more sources

Regulated autocrine growth of CHO cells.

Cytotechnology, 2000
The goal of this work was to engineer a CHO cell line capable ofautocrine growth in a fully defined protein-free medium. Thiswas accomplished by stable integration of the genes encodinginsulin-like growth factor I (IGF-I) and transferrin into thegenome of a CHO-K1 cell line. Thelac operator/repressorsystem was used to regulate the expression of the IGF-
Merilyn Sleigh, Masood Baig, Noelle-Anne Sunstrom, Sybille Marie Hunt, Sugiyono, Charles G. Bailey, Peter P. Gray   +6 more
openaire   +3 more sources

Effects of thialysine on CHO cells growth.

Microbiologica, 1985
Thialysine, the lysine isolog with the 4-methylene group substituted by a sulfur atom, inhibits the growth rate and plating efficiency of Chinese Hamster Ovary (CHO) cells. The inhibition can be reversed by lysine, when added to the culture medium together with thialysine or shortly after; to have a complete reversion a lysine concentration five times ...
M. D. Girolamo, A. D. Girolamo, COCCIA, Raffaella, C. Foppoli, BLARZINO, Carla   +4 more
openaire   +2 more sources

Dominance of colchicine resistance in hybrid CHO cells

Somatic Cell Genetics, 1978
Intraspecific hybrids of colchicine-sensitive with colchicine-resistant (CHR) Chinese hamster ovary cells were constructed, using six different colchicine-resistant clones from two independent series. In each instance, colchicine resistance was expressed in an incompletely dominant manner.
Raymond M. Baker, V. Ling
openaire   +3 more sources

The Genetic Consequences of the Thy- Mutation to CHO Cells

1985
The behavior and regulation of dNTP pools in somatic cells has long led to speculation that they may play a role in DNA synthesis besides that of simple precursors [2, 20]. While controversy has developed concerning the relationship of dNTP pools to DNA synthesis [18, 19], observations from several laboratories provide convincing evidence that pool ...
Otelinda Gonçalves, Marie Trudel, Mark Meuth   +2 more
openaire   +3 more sources

Cultivation of CHO Cells on Novel Microcarriers

2005
CHO cells were successfully cultured on a novel type of microcarriers (ImmobaSil FS) in the bioreactors. Various factors influencing cell attachment, including serum concentration, cell to microcarrier ratio, agitation speed and agitation profile were investigated and the results were compared with those obtained using CultiSpher-G, and glass beads. It
A. Y. Hu, M. Al-Rubeal, Z. Zhang
openaire   +2 more sources

A Single‐Cell Model for CHO Cellsa

Annals of the New York Academy of Sciences, 1992
P. Wu, Michael L. Shuler, N. G. Ray
openaire   +3 more sources