Results 21 to 30 of about 38,527 (251)

Mouse Adrenal Chromaffin Cell Isolation [PDF]

Journal of Visualized Experiments, 2007
Adrenal medullary chromaffin cell culture systems are extremely useful for the study of excitation-secretion coupling in an in vitro setting. This protocol illustrates the method used to dissect the adrenals and then isolate the medullary region by stripping away the adrenal cortex.
Aaron, Kolski-Andreaco, Haijiang, Cai, D Spencer, Currle, K George, Chandy, Robert H, Chow   +4 more
openaire   +2 more sources

Long-Term Viability of Isolated Bovine Adrenal Medullary Chromaffin Cells following Intrastriatal Transplantation

Cell Transplantation, 1995
Adrenal medullary grafts generally exhibit poor viability when grafted into the striatum. Previous work in our laboratory demonstrated that chromaffin cells can survive well for up to 2 mo following grafting into the intact rat striatum after cells are ...
Sherry B. Schueler, Jacqueline Sagen, George D. Pappas, Jeffrey H. Kordower   +3 more
doaj   +1 more source

Targeted Cell Reprogramming Produces Analgesic Chromaffin-Like Cells from Human Mesenchymal Stem Cells

Cell Transplantation, 2013
Transplantation of allogeneic adrenal chromaffin cells demonstrated the promise of favorable outcomes for pain relief in patients. However, there is a very limited availability of suitable human adrenal gland tissues, genetically well-matched donors in ...
T. Qu M.D., Ph.D., G. Shi, K. Ma, H. N. Yang, W. M. Duan, G. D. Pappas   +5 more
doaj   +1 more source

Hypoxia in the Initiation and Progression of Neuroblastoma Tumours [PDF]

, 2020
Neuroblastoma is the most frequent extracranial solid tumour in children, causing 10% of all paediatric oncology deaths. It arises in the embryonic neural crest due to an uncontrolled behaviour of sympathetic nervous system progenitors, giving rise to ...
Gómez Muñoz, María Ángeles, Huertas Castaño, Carlos, Pardal Redondo, Ricardo, Vega Moreno, Francisco Manuel   +3 more
core   +1 more source

Intrathecal Grafting of Porcine Chromaffin Cells Reduces Formalin-Evoked c-Fos Expression in the Rat Spinal Cord

Cell Transplantation, 2005
Chromaffin cells from the adrenal gland secrete a combination of neuroactive compounds including catecholamines, opioid peptides, and growth factors that have strong analgesic effects, especially when administered intrathecally.
J. C. Sol M.D., R. Y. Li, B. Sallerin, S. Jozan, H. Zhou, V. Lauwers-Cances, F. Tortosa, P. Chaynes, C. Mascott, R. Bastide, Y. Lazorthes   +10 more
doaj   +1 more source

Introduction of Macromolecules into Bovine Adrenal Medullary Chromaffin Cells and Rat Pheochromocytoma Cells (PC12) by Permeabilization with Streptolysin O: Inhibitory Effect of Tetanus Toxin on Catecholamine Secretion [PDF]

, 1989
Conditions are described for controlled plasma membrane permeabilization of rat pheochromocytoma cells (PC12) and cultured bovine adrenal chromaffin cells by Streptolysin O (SLO).
Ahnert-Hilger G., Ahnert-Hilger G., Ahnert-Hilger G., Bader M. F., Bhakdi S., Bhakdi S., Bhakdi S., Bittner M. A., Brooks J. C., Cazalis M., Duncan J. L., Flodgaard H., Gudrun Ahnert-Hilger, Habermann E., Hugo F., Jahn R., Knight D. E, Manfred Gratzl, Marie-France Bader, Peppers S. C., Rehm H., Sarafian T., Sillen L. G., Simon W., Smyth C. I., Sontag J. M., Sternberger A., Sucharit Bhakdi, Walton K. M., Wilson S. P.   +29 more
core   +1 more source

Initial Characterization of the Transplant of Immortalized Chromaffin Cells for the Attenuation of Chronic Neuropathic Pain

Cell Transplantation, 2000
Cultures of embryonic day 17 (E17) rat adrenal and neonatal bovine adrenal cells were conditionally immortalized with the temperature-sensitive allele of SV40 large T antigen (tsTag) and chromaffin cell lines established.
M. J. Eaton Ph.D., M. Martinez, S. Karmally, T. Lopez, J. Sagen   +4 more
doaj   +1 more source

Chromaffin cells: the peripheral brain [PDF]

Molecular Psychiatry, 2012
Chromaffin cells probably are the most intensively studied of the neural crest derivates. They are closely related to the nervous system, share with neurons some fundamental mechanisms and thus were the ideal model to study the basic mechanisms of neurobiology for many years.
Bornstein, SR, Ehrhart-Bornstein, Monika, Androutsellis-Theotokis, A, Eisenhofer, G, Vukicevic, V, Licinio, Julio, Wong, Ma-Li, Calissano, P, Nistico, G, Preziosi, P, Levi-Montalcini, R   +10 more
openaire   +5 more sources

Matrix-free calcium in isolated chromaffin vesicles [PDF]

, 1985
Isolated secretory vesicles from bovine adrenal medulla contain 80 nmol of Ca2+ and 25 nmol of Mg2+ per milligram of protein. As determined with a Ca2+-selective electrode, a further accumulation of about 160 nmol of Ca2+/mg of protein can be attained ...
Bashford L. C., Borowitz J. L., Borowitz J. L., Bradford M., Coll K. E., Debono M., Department of Biochemistry, Flodgaard H., Gratzl M., Grinstein S., Hansford R. G., Johnson R. G., Joseph S. K., Knight D. E., Krieger-Brauer H., Krieger-Brauer H., Krieger-Brauer H., Lowry O. H., MacLennan D. M., Nordmann J. J., Pfeiffer D. R., Pfeiffer D. R., Phillips J. H., Phillips J. H., Pollard B., Pollard B., Ravazzola M., Reed P. W., Reed P. W., Reinhart P. H., Saermark T., Saermark T., Serck-Hanssen G., Sillen L. G., Simon W., Wada A.   +35 more
core   +2 more sources

Immunohistochemical evidence for the presence of a vasoactive intestinal peptide, neuropeptide Y and substance P in rat adrenal medulla after heat stress [PDF]

Archives of Biological Sciences, 2012
Immunohistochemistry revealed the presence of VIP-, NPY- and SP-immunoreactivity in the rat adrenal medulla. VIP- and NPY-immunoreactivity was detected in chromaffin and ganglion cells and in nerve fibers, but SP-immunoreactivity was found only in ...
Petrović-Kosanović Dragana, Koko Vesna
doaj   +1 more source