Results 31 to 40 of about 32,833 (259)
Chromogranins, widespread in endocrine and nervous tissue, bind Ca2+ [PDF]
The proteinaceous components of the secretory vesicle contents isolated from bovine adrenal medulla bind Ca2+ (number of binding sites, 152 ± 52 nmol Ca2+ per mg protein; dissociation constant, 54 ± 8 μM (n = 5)).
Gratzl, Manfred, Reiffen, F. U.
core +1 more source
Adrenal chromaffin cells have been utilized for several neural grafting applications, but limitations in allogeneic donor availability and dangers inherent in auto-grafting limit the widespread use of this approach clinically.
Kimberly A. Czech+3 more
doaj +1 more source
Ca2+-Stimulated Catecholamine Release from alpha-Toxin Permeabilized PC12 Cells [PDF]
Two possible cellular pathways of catecholamines from the chromaffin vesicles of PC 12 cells to the surrounding medium are explored in this study. The direct one circumventing the cytoplasm can be activated in a-toxin-permeabilized cells with micromolar ...
Ahnert-Hilger, G.+2 more
core +1 more source
We have found that immunosuppression is necessary for the survival of xenogeneic adrenal medullary transplants. Because chromaffin cells are essentially nonimmunogenic, it is likely that the highly immunogenic “passenger” cells in the transplant ...
P. Michalewicz+4 more
doaj +1 more source
Molecular Aspects of Secretory Granule Exocytosis by Neurons and Endocrine Cells [PDF]
Neuronal communication and endocrine signaling are fundamental for integrating the function of tissues and cells in the body. Hormones released by endocrine cells are transported to the target cells through the circulation.
Ahnert-Hilger G.+28 more
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GABA signaling and neuroactive steroids in adrenal medullary chromaffin cells
GABA is produced not only in the brain, but also in endocrine cells by the two isoforms of glutamic acid decarboxylase (GAD), GAD65 and GAD67. In rat adrenal medullary chromaffin cells only GAD67 is expressed, and GABA is stored in large dense core ...
Keita eHarada+5 more
doaj +1 more source
Microelectrode arrays of diamond-insulated graphitic channels for real time detection of exocytotic events from cultured chromaffin cells and slices of adrenal glands [PDF]
A microstructured graphitic 4x4 multielectrode array was embedded in a single crystal diamond substrate (4x4 {uG-SCD MEA) for real-time monitoring of exocytotic events from cultured chromaffin cells and adrenal slices.
Battiato, A.+7 more
core +3 more sources
Mouse Adrenal Chromaffin Cell Isolation [PDF]
Adrenal medullary chromaffin cell culture systems are extremely useful for the study of excitation-secretion coupling in an in vitro setting. This protocol illustrates the method used to dissect the adrenals and then isolate the medullary region by stripping away the adrenal cortex.
D. Spencer Currle+4 more
openaire +3 more sources
Immunological characterization of chromogranins A and B and secretogranin II in the bovine pancreatic islet [PDF]
Antisera against chromogranin A and B and secretogranin II were used for analysing the bovine pancreas by immunoblotting and immunohistochemistry. All three antigens were found in extracts of fetal pancreas by one dimensional immunoblotting. A comparison
A Iacangelo+44 more
core +1 more source
Generation of Adrenal Chromaffin-like Cells from Human Pluripotent Stem Cells
Summary: Adrenomedullary chromaffin cells are catecholamine (CA)-producing cells originating from trunk neural crest (NC) via sympathoadrenal progenitors (SAPs). We generated NC and SAPs from human embryonic stem cells (ESCs) and induced pluripotent stem
Kwaku Dad Abu-Bonsrah+4 more
doaj +1 more source