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The European Physical Journal E, 2006
Nucleosome is the basic subunit of the chromatin, which organizes the genomic DNA within the cell nucleus. It was understood in the last decade that beside the DNA compaction it plays an important role in the regulation of the gene expression. In its intact form, the nucleosome represents an important mechanical barrier and, among others, it prevents ...
C, Claudet, J, Bednar
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Nucleosome is the basic subunit of the chromatin, which organizes the genomic DNA within the cell nucleus. It was understood in the last decade that beside the DNA compaction it plays an important role in the regulation of the gene expression. In its intact form, the nucleosome represents an important mechanical barrier and, among others, it prevents ...
C, Claudet, J, Bednar
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International Journal for Parasitology, 1994
The nuclear chromatin of trypanosomes is organised in the form of nucleosome filaments. When soluble chromatin is prepared under suitable conditions, a regular array of nucleosomes can be shown by electron microscopy. Chromatin of blood stream as well as procyclic culture forms of Trypanosoma brucei brucei and of T.
H, Hecker +4 more
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The nuclear chromatin of trypanosomes is organised in the form of nucleosome filaments. When soluble chromatin is prepared under suitable conditions, a regular array of nucleosomes can be shown by electron microscopy. Chromatin of blood stream as well as procyclic culture forms of Trypanosoma brucei brucei and of T.
H, Hecker +4 more
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Cold Spring Harbor Protocols, 2020
Chromatin immunoprecipitation, commonly referred to as ChIP, is a powerful technique for the evaluation of in vivo interactions of proteins with specific regions of genomic DNA. Formaldehyde is used in this technique to cross-link proteins to DNA in vivo, followed by the extraction of chromatin from cross-linked cells and tissues.
James, DeCaprio, Thomas O, Kohl
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Chromatin immunoprecipitation, commonly referred to as ChIP, is a powerful technique for the evaluation of in vivo interactions of proteins with specific regions of genomic DNA. Formaldehyde is used in this technique to cross-link proteins to DNA in vivo, followed by the extraction of chromatin from cross-linked cells and tissues.
James, DeCaprio, Thomas O, Kohl
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Current Opinion in Plant Biology, 2001
Epigenetic mechanisms are heritable traits that are mediated by changes in a genetic locus that do not involve a modification at the nucleotide level. As eukaryotic DNA is organised in chromatin units, epigenetic modifications can be mediated by chromatin remodelling.
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Epigenetic mechanisms are heritable traits that are mediated by changes in a genetic locus that do not involve a modification at the nucleotide level. As eukaryotic DNA is organised in chromatin units, epigenetic modifications can be mediated by chromatin remodelling.
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2013
Recent studies have elucidated molecular mechanisms underlying the transcriptional control of metabolism in complex metabolic disorders such as metabolic syndrome and atherosclerosis. Chromatin immunoprecipitation (ChIP) is an important technique to study protein-DNA interactions in vivo.
Grant D, Barish, Rajenda K, Tangirala
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Recent studies have elucidated molecular mechanisms underlying the transcriptional control of metabolism in complex metabolic disorders such as metabolic syndrome and atherosclerosis. Chromatin immunoprecipitation (ChIP) is an important technique to study protein-DNA interactions in vivo.
Grant D, Barish, Rajenda K, Tangirala
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Immunoprecipitation of chromatin
1996Publisher Summary This chapter discusses the immunoprecipitation of chromatin. The chapter discusses three standard protocols, all of which are used to analyze the role of specific proteins within defined regions of the eukaryotic genome. It analyzes both the DNA and the protein after immunoprecipitation.
L P, O'Neill, B M, Turner
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The FASEB Journal, 1996
The compaction of DNA into chromatin in the eukaryotic nucleus poses many obstacles to transcription. Individual nucleosomes as well as higher order structures limit access of cis‐acting regulatory elements to trans‐acting factors.
D G, Edmondson, S Y, Roth
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The compaction of DNA into chromatin in the eukaryotic nucleus poses many obstacles to transcription. Individual nucleosomes as well as higher order structures limit access of cis‐acting regulatory elements to trans‐acting factors.
D G, Edmondson, S Y, Roth
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Nature New Biology, 1971
Physico-chemical experiments show that histones are not evenly distributed in chromatin. About half of the DNA is “open” and not covered with proteins.
R J, Clark, G, Felsenfeld
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Physico-chemical experiments show that histones are not evenly distributed in chromatin. About half of the DNA is “open” and not covered with proteins.
R J, Clark, G, Felsenfeld
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Annual Review of Genetics, 2006
Active and silenced chromatin domains are often in close juxtaposition to one another, and enhancer and silencer elements operate over large distances to regulate the genes in these domains. The lack of promiscuity in the function of these elements suggests that active mechanisms exist to restrict their activity.
Lourdes, Valenzuela +1 more
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Active and silenced chromatin domains are often in close juxtaposition to one another, and enhancer and silencer elements operate over large distances to regulate the genes in these domains. The lack of promiscuity in the function of these elements suggests that active mechanisms exist to restrict their activity.
Lourdes, Valenzuela +1 more
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2016
Chromatin immunoprecipitation (ChIP) is a valuable method to investigate protein-DNA interactions in vivo. Since its discovery it has been indispensable to identify binding sites and patterns of a variety of DNA-interacting proteins, such as transcription factors and regulators, modified histones, and epigenetic modifiers.
Laura, Wiehle, Achim, Breiling
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Chromatin immunoprecipitation (ChIP) is a valuable method to investigate protein-DNA interactions in vivo. Since its discovery it has been indispensable to identify binding sites and patterns of a variety of DNA-interacting proteins, such as transcription factors and regulators, modified histones, and epigenetic modifiers.
Laura, Wiehle, Achim, Breiling
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