Results 141 to 150 of about 1,193,888 (281)

Purification and Characterization of α-Amylase from <i>Bacillus simplex</i> BCHCNZ282B. [PDF]

Curr Issues Mol Biol
Karakoç N, Fincan SA, Enez B, Ortakaya V.   +3 more
europepmc   +1 more source

A Single‐Cell Transcriptomic Atlas of the Ovine Rumen Microbiome Characterizes Lineage‐Specific Metabolic Shifts Associated with Host Heat Tolerance

Advanced Science, EarlyView.
An optimized single‐cell transcriptomic framework profiles over 60 000 cells to map the ovine rumen microbiome, partitioning the ecosystem into seven cross‐species functional clusters. In heat‐resistant hosts, a lineage‐specific metabolic shift in Anaerovibrio lipolyticus toward a highly glycolytic phenotype contributes to a “nutritional sparing ...
Sanbao Zhang, Qinyang Jiang, Junjie Ma, Jianwei Zou, Fan Wang, Feifei Lv, Yanna Huang, Yongcheng Wang, Ziye Xu   +8 more
wiley   +1 more source

Removal of Empty AAV Capsids to Undetectable Levels Using Orthogonal Purification Steps. [PDF]

Biotechnol Bioeng
Sheth RD, Boutigny S, Perez J, Silberstein D, Zhang Y, Karim O, Gil G, Rishi A, Vora H, Gold D, Maga J.   +10 more
europepmc   +1 more source

Radiation‐Induced Tumor‐Intrinsic LTβR N‐Glycosylation Suppresses Pyroptosis Through TRIM28‐Mediated PCBP2 SUMOylation to Promote Gastric Cancer Radioresistance

Advanced Science, EarlyView.
Radiotherapy triggers LTβR N‐glycosylation, enhancing its overall protein stability and nuclear retention. This accumulation drives TRIM28‐mediated PCBP2 SUMOylation, suppressing pyroptosis and conferring gastric cancer radioresistance. Therapeutically, a targeted nanoplatform (cRGD‐Lipo@EMD) effectively disrupts this regulatory axis, offering a highly
Weijie Zang, Yunlong Ji, Chunwang Zhou, Xiwen Li, Zhuang Lu, Guangze Zhang, Yu Chen, Wanjiang Xue, Yilin Hu   +8 more
wiley   +1 more source

Fractionation and analysis of Artemisia capillaris Thunb. by affinity chromatography with human serum albumin as stationary phase

, 2000
A method for the screening and analysis of biologically active compounds in traditional Chinese medicine is proposed. Affinity chromatography using a human serum albumin (HSA) stationary phase was applied to separate and analyze the bioactive compounds ...
Kong, L, Zou, HF, Guo, BC, Ni, JY, Gao, S, Wang, HL   +5 more
core  

Affinity purification contaminants identified by cryo-EM and mass spectrometry. [PDF]

Biosci Rep
Belcher ER, Hardwick SW, Maia de Oliveira T, Hyvönen M.   +3 more
europepmc   +1 more source

Tandem Duplication‐Driven Neofunctionalization of UDP‐Glycosyltransferases Shapes the Diversification of Triterpenoid Saponins in the Cucurbitaceae

Advanced Science, EarlyView.
We present a chromosome‐level genome assembly of Siraitia grosvenorii and, through comparative genomics, uncover a conserved UGT73 tandem array driving triterpenoid saponin diversification in Cucurbitaceae. Crystalized SgUGT73AM30 further reveals the regioselectivity mechanism underlying its catalytic activity.
Guangyi Wang, Mengmeng Li, Xuehui Dai, Yanchen Zhang, Yuhan Wu, Zhaotao Yan, Chenfei Tian, Hongkai Fan, Haili Liu, Xiaowei Zhang, Yiming Yang, Jiayu Lu, Yuwei Sun, Yong Wang   +13 more
wiley   +1 more source

The Content and Location of 3-<i>O</i>-Sulfated Glucosamine Enable Differentiation of Ovine, Bovine and Porcine Heparins. [PDF]

Biomolecules
Somma A, Urso E, Parafioriti M, Guerrini M, Bisio A.   +4 more
europepmc   +1 more source

Conformational Transduction Amplification in a Biomimetic Polyelectrolyte for Ultrasensitive Imaging of Iron Metabolism

Advanced Science, EarlyView.
This work designs a peptide‐free polyelectrolyte with stable, switchable conformations and near‐infrared fluorescence in water. It harnesses a cooperative Fe3+‐triggered sheet‐to‐helix transition to generate nonlinear fluorescence quenching, enabling the ultrasensitive, real‐time visualization of Fe3+ ion distribution and metabolic trafficking at ...
Yeqiang Zhou, Danqi Yang, Yiwei Wang, Shuangyan Li, Fan Fan, Jiayu Zou, Cheng Zhang, Yang Liu, Hong Tan, Mingming Ding   +9 more
wiley   +1 more source

Facile, high-throughput protein purification enabled by high-efficiency protease elution. [PDF]

Protein Sci
Cárcamo Noriega EN, Truebridge IS, Teets FD, Bowman JW, Rodriguez N, Howard TA, Bahl CD.   +6 more
europepmc   +1 more source