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Removal of dsRNA byproducts using affinity chromatography [PDF]

Molecular Therapy: Nucleic Acids
Double-stranded RNA (dsRNA) molecules are immunogenic byproducts of in vitro transcription of single-stranded RNA (ssRNA). Removal of dsRNA from ssRNA is difficult because the byproducts have similar sizes, sequences, and charges to the desired ssRNA ...
Nathaniel E. Clark, Mateusz Kozarski, Sinem Demirel Asci, Jurgen Van den Heuvel, Matt R. Schraut, Roger A. Winters, Kelley Kearns, Thomas C. Scanlon, Senne Dillen   +8 more
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Kinetic Analysis by Affinity Chromatography [PDF]

Frontiers in Chemistry, 2019
Important information on chemical processes in living systems can be obtained by the rates at which these biological interactions occur. This review will discuss several techniques based on traditional and high-performance affinity chromatography that ...
Sazia Iftekhar, Susan T. Ovbude, David S. Hage   +2 more
doaj   +2 more sources

High-efficiency purification of divergent AAV serotypes using AAVX affinity chromatography [PDF]

Molecular Therapy: Methods & Clinical Development, 2023
The adeno-associated viral vector (AAV) provides a safe and efficient gene therapy platform with several approved products that have marked therapeutic impact for patients.
Michael Florea, Fotini Nicolaou, Simon Pacouret, Eric M. Zinn, Julio Sanmiguel, Eva Andres-Mateos, Carmen Unzu, Amy J. Wagers, Luk H. Vandenberghe   +8 more
doaj   +2 more sources

Extending the Affinity Range of Weak Affinity Chromatography for the Identification of Weak Ligands Targeting Membrane Proteins [PDF]

Molecules, 2023
The identification of weak-affinity ligands targeting membrane proteins is of great interest in Fragment-Based Drug Design (FBDD). Recently, miniaturized weak affinity chromatography (WAC) has been proposed as a valuable tool to study interactions ...
Adrien Deloche, François-Xavier Vidal, Lucile Jammas, Renaud Wagner, Vincent Dugas, Claire Demesmay   +5 more
doaj   +2 more sources

Ligand Screening of Membrane Proteins Embedded in Nanodiscs: How to Manage Non-Specific Interactions in Weak Affinity Chromatography? [PDF]

Molecules
Miniaturized weak affinity chromatography is emerging as an interesting alternative to conventional biophysical tools for performing fragment-screening studies in the context of fragment-based drug discovery.
François-Xavier Vidal, Adrien Deloche, Gabrielle Zeder-Lutz, Maria Hideux, Renaud Wagner, Vincent Dugas, Claire Demesmay   +6 more
doaj   +2 more sources

Snorkel‐tag based affinity chromatography for recombinant extracellular vesicle purification [PDF]

Journal of Extracellular Vesicles
Extracellular vesicles (EVs) are lipid nanoparticles and play an important role in cell‐cell communications, making them potential therapeutic agents and allowing to engineer for targeted drug delivery.
Madhusudhan Reddy Bobbili, André Görgens, Yan Yan, Stefan Vogt, Dhanu Gupta, Giulia Corso, Samir Barbaria, Carolina Patrioli, Sylvia Weilner, Marianne Pultar, Jaroslaw Jacak, Matthias Hackl, Markus Schosserer, Regina Grillari, Jørgen Kjems, Samir EL Andaloussi, Johannes Grillari   +16 more
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Affinity chromatography: A review of trends and developments over the past 50 years [PDF]

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 2020
Kyungah Suh, David S Hage
exaly   +2 more sources

Affinity and Pseudo-Affinity Membrane Chromatography for Viral Vector and Vaccine Purifications: A Review

Membranes, 2023
Several chromatographic approaches have been established over the last decades for the production of pharmaceutically relevant viruses. Due to the large size of these products compared to other biopharmaceuticals, e.g., proteins, convective flow media ...
Keven Lothert, Michael W. Wolff
doaj   +1 more source

Lectin Purification through Affinity Chromatography Exploiting Macroporous Monolithic Adsorbents

Separations, 2023
Growing medical, engineering, biochemical, and biological interest has led to a steady pace of research and development into polymeric monolithic structures with densely interconnected pores for purifying bio compounds.
Josiane F. da Silva, Clara M. G. Lima, Débora L. da Silva, Ivonea S. do Nascimento, Sarah de O. Rodrigues, Letícia A. Gonçalves, Renata F. Santana, Waseem Khalid, Silvani Verruck, Talha Bin Emran, Irwin R. A. de Menezes, Henrique D. M. Coutinho, Mayeen U. Khandaker, Mohammad R. I. Faruque, Rafael da C. I. Fontan   +14 more
doaj   +1 more source

RNA-Affinity Chromatography

Bio-Protocol, 2013
RNA-affinity chromatography assays are used to identify proteins binding specific RNA sequences. These proteins represent potential factors contributing to the function of RNA molecules. In our lab, we have used this protocol to identify proteins binding
Lucia Morales , Pedro Mateos-Gomez, Luis Enjuanes, Isabel Sola   +3 more
doaj   +1 more source