Results 1 to 10 of about 936,786 (259)
Nature, 1990 Weak-affinity chromatography is a new method using readily reversible biospecific recognition as the basis for chromatographic separations.
Sten Ohlson, David Zopf
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Membranes, 2023 Several chromatographic approaches have been established over the last decades for the production of pharmaceutically relevant viruses. Due to the large size of these products compared to other biopharmaceuticals, e.g., proteins, convective flow media ...
Keven Lothert, Michael W. Wolff
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Lectin Purification through Affinity Chromatography Exploiting Macroporous Monolithic Adsorbents
Separations, 2023 Growing medical, engineering, biochemical, and biological interest has led to a steady pace of research and development into polymeric monolithic structures with densely interconnected pores for purifying bio compounds.
Josiane F. da Silva +14 more
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Bio-Protocol, 2013 RNA-affinity chromatography assays are used to identify proteins binding specific RNA sequences. These proteins represent potential factors contributing to the function of RNA molecules. In our lab, we have used this protocol to identify proteins binding
Lucia Morales +3 more
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Kinetic Analysis by Affinity Chromatography
Frontiers in Chemistry, 2019 Important information on chemical processes in living systems can be obtained by the rates at which these biological interactions occur. This review will discuss several techniques based on traditional and high-performance affinity chromatography that ...
Sazia Iftekhar +2 more
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Glycoform-resolved FcɣRIIIa affinity chromatography–mass spectrometry
mAbs, 2019 Determination of the impact of individual antibody glycoforms on FcɣRIIIa affinity, and consequently antibody-dependent cell-mediated cytotoxicity (ADCC) previously required high purity glycoengineering.
Steffen Lippold +8 more
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The use of phenyl-Sepharose for the affinity purification of proteinases [PDF]
, 1993 Phenyl-Sepharose is most often used as an adsorbent for hydrophobic interaction chromatography (HIC). We report on its effective use for the affinity purification of some extracellular thermostable proteinases from bacterial sources.
Prescott, Mark +7 more
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Dimerization capacities of FGF2 purified with or without heparin-affinity chromatography. [PDF]
PLoS ONE, 2014 Fibroblast growth factor-2 (FGF2) is a pleiotropic growth factor exhibiting a variety of biological activities. In this article, we studied the capacity of FGF2 purified with or without heparin affinity chromatography to self-associate. Analyzing the NMR
Natalia Platonova +8 more
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Screening Tagged Proteins Using Tandem Affinity-Buffer Exchange Chromatography Online with Native Mass Spectrometry [PDF]
, 2021 Protein overexpression and purification are critical for in vitro structure-function characterization studies. However, some proteins are difficult to express robustly in heterologous systems due to host-related (e.g., codon usage, translation rate) and ...
Vicki, Wysocki +4 more
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Affinity Chromatography of Lipoxygenases [PDF]
European Journal of Biochemistry, 1977 A number of aminohexyl agarose derivatives of unsaturated fatty acids have been prepared and evaluated as materials for the affinity chromatography of soybean and pea lipoxygenases. A practical method for a one-stage purification of soybean lipoxygenase-1, with a purification factor of 16, is described, using either linolenate or docosa-4,7,10,13,16,19-
J C, Allen, C, Eriksson, J R, Galpin
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