Glycoform-resolved FcɣRIIIa affinity chromatography–mass spectrometry
mAbs, 2019 Determination of the impact of individual antibody glycoforms on FcɣRIIIa affinity, and consequently antibody-dependent cell-mediated cytotoxicity (ADCC) previously required high purity glycoengineering.
Steffen Lippold +8 more
doaj +4 more sources
Affinity chromatography: A review of trends and developments over the past 50 years. [PDF]
J Chromatogr B Analyt Technol Biomed Life Sci, 2020 The field of affinity chromatography, which employs a biologically-related agent as the stationary phase, has seen significant growth since the modern era of this method began in 1968.
Rodriguez EL +9 more
europepmc +4 more sources
Removal of dsRNA byproducts using affinity chromatography [PDF]
Molecular Therapy: Nucleic AcidsDouble-stranded RNA (dsRNA) molecules are immunogenic byproducts of in vitro transcription of single-stranded RNA (ssRNA). Removal of dsRNA from ssRNA is difficult because the byproducts have similar sizes, sequences, and charges to the desired ssRNA ...
Nathaniel E. Clark +8 more
doaj +2 more sources
High-efficiency purification of divergent AAV serotypes using AAVX affinity chromatography [PDF]
Molecular Therapy: Methods & Clinical Development, 2023 The adeno-associated viral vector (AAV) provides a safe and efficient gene therapy platform with several approved products that have marked therapeutic impact for patients.
Michael Florea +8 more
doaj +2 more sources
Kinetic Analysis by Affinity Chromatography [PDF]
Frontiers in Chemistry, 2019 Important information on chemical processes in living systems can be obtained by the rates at which these biological interactions occur. This review will discuss several techniques based on traditional and high-performance affinity chromatography that ...
Sazia Iftekhar +2 more
doaj +2 more sources
Extending the Affinity Range of Weak Affinity Chromatography for the Identification of Weak Ligands Targeting Membrane Proteins [PDF]
Molecules, 2023 The identification of weak-affinity ligands targeting membrane proteins is of great interest in Fragment-Based Drug Design (FBDD). Recently, miniaturized weak affinity chromatography (WAC) has been proposed as a valuable tool to study interactions ...
Adrien Deloche +5 more
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Ligand Screening of Membrane Proteins Embedded in Nanodiscs: How to Manage Non-Specific Interactions in Weak Affinity Chromatography? [PDF]
MoleculesMiniaturized weak affinity chromatography is emerging as an interesting alternative to conventional biophysical tools for performing fragment-screening studies in the context of fragment-based drug discovery.
François-Xavier Vidal +6 more
doaj +2 more sources
Snorkel‐tag based affinity chromatography for recombinant extracellular vesicle purification [PDF]
Journal of Extracellular VesiclesExtracellular vesicles (EVs) are lipid nanoparticles and play an important role in cell‐cell communications, making them potential therapeutic agents and allowing to engineer for targeted drug delivery.
Madhusudhan Reddy Bobbili +16 more
doaj +2 more sources
Antibody Fragments and Their Purification by Protein L Affinity Chromatography
Antibodies, 2015 Antibodies and related proteins comprise one of the largest and fastest-growing classes of protein pharmaceuticals. A majority of such molecules are monoclonal antibodies; however, many new entities are antibody fragments.
Gustav Rodrigo +2 more
doaj +2 more sources
High-grade extracellular vesicles preparation by combined size-exclusion and affinity chromatography [PDF]
Scientific Reports, 2021 Extracellular vesicles (EVs) have recently gained growing interest for their diagnostic and therapeutic potential. Despite this, few protocols have been reported for the isolation of EVs with preserved biological function.
Cristina Bellotti +4 more
doaj +2 more sources