Results 11 to 20 of about 2,190,614 (293)

Selection of imprinted nanoparticles by affinity chromatography [PDF]

Biosensors and Bioelectronics, 2009
Soluble molecularly imprinted nanoparticles were synthesised via iniferter initiated polymerisation and separated by size via gel permeation chromatography.
Chianella, Iva, Guerreiro, A., Piletska, Elena V., Piletsky, Sergey A., Whitcombe, M. J.   +4 more
core   +3 more sources

Affinity chromatography for vaccines manufacturing: Finally ready for prime time?

Vaccine, 2019
Affinity chromatography is among the most powerful separation techniques, achieving the finest separation with high yields even in the most challenging feed streams. Incorporating affinity chromatography in vaccine purification has long been attempted by
Mochao Zhao, Melissa Vandersluis, James Stout, U. Haupts, M. Sanders, R. Jacquemart   +5 more
semanticscholar   +3 more sources

Protein purification by affinity chromatography [PDF]

, 2012
Affinity chromatography is a method which depends essentially on the interaction between the molecule to be purified and a solid phase that will allow the separation of contaminants.
Coelho, L. C. B. B., Correia, M. T. S., Napoleão, Thiago H., Paiva, Patrícia M. G., Santos, Andréa F. S.   +4 more
core   +2 more sources

Affinity monolith chromatography: A review of general principles and recent developments

Electrophoresis, 2021
Affinity monolith chromatography (AMC) is a liquid chromatographic technique that utilizes a monolithic support with a biological ligand or related binding agent to isolate, enrich, or detect a target analyte in a complex matrix.
S. Poddar, Sadia Sharmeen, D. Hage
semanticscholar   +1 more source

Affinity and Pseudo-Affinity Membrane Chromatography for Viral Vector and Vaccine Purifications: A Review

Membranes, 2023
Several chromatographic approaches have been established over the last decades for the production of pharmaceutically relevant viruses. Due to the large size of these products compared to other biopharmaceuticals, e.g., proteins, convective flow media ...
Keven Lothert, Michael W. Wolff
doaj   +1 more source

Immobilized metal affinity chromatography optimization for poly-histidine tagged proteins.

Journal of Chromatography A, 2020
Immobilized metal affinity chromatography (IMAC) is a technique primarily used in research and development laboratories to purify proteins containing engineered histidine tags.
Valeria Riguero, R. Clifford, Michael Dawley, M. Dickson, Benjamin D. Gastfriend, Christopher Thompson, Sheau-Chiann Wang, Ellen O'Connor   +7 more
semanticscholar   +1 more source

Pharmaceutical and biomedical applications of affinity chromatography: recent trends and developments.

Journal of Pharmaceutical and Biomedical Analysis, 2012
D. Hage, Jeanethe A Anguizola, Cong Bi, Rong Li, Ryan Matsuda, E. Papastavros, E. Pfaunmiller, J. Vargas, Xiwei Zheng   +8 more
semanticscholar   +3 more sources

Lectin Purification through Affinity Chromatography Exploiting Macroporous Monolithic Adsorbents

Separations, 2023
Growing medical, engineering, biochemical, and biological interest has led to a steady pace of research and development into polymeric monolithic structures with densely interconnected pores for purifying bio compounds.
Josiane F. da Silva, Clara M. G. Lima, Débora L. da Silva, Ivonea S. do Nascimento, Sarah de O. Rodrigues, Letícia A. Gonçalves, Renata F. Santana, Waseem Khalid, Silvani Verruck, Talha Bin Emran, Irwin R. A. de Menezes, Henrique D. M. Coutinho, Mayeen U. Khandaker, Mohammad R. I. Faruque, Rafael da C. I. Fontan   +14 more
doaj   +1 more source

RNA-Affinity Chromatography

Bio-Protocol, 2013
RNA-affinity chromatography assays are used to identify proteins binding specific RNA sequences. These proteins represent potential factors contributing to the function of RNA molecules. In our lab, we have used this protocol to identify proteins binding
Lucia Morales , Pedro Mateos-Gomez, Luis Enjuanes, Isabel Sola   +3 more
doaj   +1 more source

Purification of Mg2+-dependent phosphatidate phosphohydrolase from rat liver: new steps and aspects [PDF]

, 2005
A new procedure for the partial purification of Mg2+-dependent, N-ethylmaleimide-sensitive phosphatidate phosphohydrolase (Mg2+-PAP; EC 3.1.3.4) from rat liver cytosol is described, using protein precipitation with MgCl2, gel filtration on Sephacryl S ...
Butterwith, Cascales, DeMattos, Hopewell, Hosaka, Martin, Mavis, Minami, Nemoto, Nishida, Pollard   +10 more
core   +1 more source