Results 11 to 20 of about 1,195,639 (276)

Monoclonal antibody (mAb) purification by counter current chromatography (CCC) [PDF]

open access: yes, 2011
This thesis was submitted for the degree of Doctor of Philosophy and awarded by Brunel University.Counter current chromatography (CCC) is a form of liquid liquid chromatography, which the Brunel Institute for Bioengineering (BIB) team have developed to ...
Fernando, Samantha
core   +7 more sources

Glycoform-resolved FcɣRIIIa affinity chromatography–mass spectrometry

open access: yesmAbs, 2019
Determination of the impact of individual antibody glycoforms on FcɣRIIIa affinity, and consequently antibody-dependent cell-mediated cytotoxicity (ADCC) previously required high purity glycoengineering.
Steffen Lippold   +8 more
doaj   +1 more source

Dimerization capacities of FGF2 purified with or without heparin-affinity chromatography. [PDF]

open access: yesPLoS ONE, 2014
Fibroblast growth factor-2 (FGF2) is a pleiotropic growth factor exhibiting a variety of biological activities. In this article, we studied the capacity of FGF2 purified with or without heparin affinity chromatography to self-associate. Analyzing the NMR
Natalia Platonova   +8 more
doaj   +1 more source

Affinity Chromatography of Lipoxygenases [PDF]

open access: yesEuropean Journal of Biochemistry, 1977
A number of aminohexyl agarose derivatives of unsaturated fatty acids have been prepared and evaluated as materials for the affinity chromatography of soybean and pea lipoxygenases. A practical method for a one-stage purification of soybean lipoxygenase-1, with a purification factor of 16, is described, using either linolenate or docosa-4,7,10,13,16,19-
J C, Allen, C, Eriksson, J R, Galpin
openaire   +2 more sources

The use of phenyl-Sepharose for the affinity purification of proteinases [PDF]

open access: yes, 1993
Phenyl-Sepharose is most often used as an adsorbent for hydrophobic interaction chromatography (HIC). We report on its effective use for the affinity purification of some extracellular thermostable proteinases from bacterial sources.
Prescott, Mark   +3 more
core   +1 more source

Purification of baculovirus vectors using heparin affinity chromatography

open access: yesMolecular Therapy: Methods & Clinical Development, 2016
Baculoviruses are commonly used for recombinant protein and vaccine production. Baculoviruses are nonpathogenic to vertebrates, have a large packaging capacity, display broad host and cell type tropism, infect both dividing and nondividing cells, and do ...
Md Nasimuzzaman   +3 more
doaj   +1 more source

Non-targeted characterization of attributes affecting antibody-FcγRIIIa V158 (CD16a) binding via online affinity chromatography-mass spectrometry

open access: yesmAbs, 2022
Antibodies facilitate targeted cell killing by engaging with immune cells such as natural killer cells through weak binding interactions with Fcγ receptors on the cell surface.
Daniel W. Woodall   +6 more
doaj   +1 more source

Enhanced production of recombinant HALT-1 pore-forming toxin using two-step chromatographic procedure

open access: yesMethodsX, 2023
Hydra actinoporin-like toxin-1 (HALT-1) has been isolated from Hydra magnipapillata and is highly cytolytic against various human cells including erythrocyte.
Wei Yuen Yap   +3 more
doaj   +1 more source

Continuous Affinity Purification of Adeno-Associated Virus Using Periodic Counter-Current Chromatography

open access: yesPharmaceutics, 2022
Replacing batch unit operations of biopharmaceuticals by continuous manufacturing is a maturing concept, with periodic counter-current chromatography (PCC) favoured to replace batch chromatography.
João P. Mendes   +5 more
doaj   +1 more source

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