Results 11 to 20 of about 1,195,639 (276)
Monoclonal antibody (mAb) purification by counter current chromatography (CCC) [PDF]
This thesis was submitted for the degree of Doctor of Philosophy and awarded by Brunel University.Counter current chromatography (CCC) is a form of liquid liquid chromatography, which the Brunel Institute for Bioengineering (BIB) team have developed to ...
Fernando, Samantha
core +7 more sources
Glycoform-resolved FcɣRIIIa affinity chromatography–mass spectrometry
Determination of the impact of individual antibody glycoforms on FcɣRIIIa affinity, and consequently antibody-dependent cell-mediated cytotoxicity (ADCC) previously required high purity glycoengineering.
Steffen Lippold +8 more
doaj +1 more source
Dimerization capacities of FGF2 purified with or without heparin-affinity chromatography. [PDF]
Fibroblast growth factor-2 (FGF2) is a pleiotropic growth factor exhibiting a variety of biological activities. In this article, we studied the capacity of FGF2 purified with or without heparin affinity chromatography to self-associate. Analyzing the NMR
Natalia Platonova +8 more
doaj +1 more source
Affinity Chromatography of Lipoxygenases [PDF]
A number of aminohexyl agarose derivatives of unsaturated fatty acids have been prepared and evaluated as materials for the affinity chromatography of soybean and pea lipoxygenases. A practical method for a one-stage purification of soybean lipoxygenase-1, with a purification factor of 16, is described, using either linolenate or docosa-4,7,10,13,16,19-
J C, Allen, C, Eriksson, J R, Galpin
openaire +2 more sources
The use of phenyl-Sepharose for the affinity purification of proteinases [PDF]
Phenyl-Sepharose is most often used as an adsorbent for hydrophobic interaction chromatography (HIC). We report on its effective use for the affinity purification of some extracellular thermostable proteinases from bacterial sources.
Prescott, Mark +3 more
core +1 more source
Purification of baculovirus vectors using heparin affinity chromatography
Baculoviruses are commonly used for recombinant protein and vaccine production. Baculoviruses are nonpathogenic to vertebrates, have a large packaging capacity, display broad host and cell type tropism, infect both dividing and nondividing cells, and do ...
Md Nasimuzzaman +3 more
doaj +1 more source
Antibodies facilitate targeted cell killing by engaging with immune cells such as natural killer cells through weak binding interactions with Fcγ receptors on the cell surface.
Daniel W. Woodall +6 more
doaj +1 more source
Hydra actinoporin-like toxin-1 (HALT-1) has been isolated from Hydra magnipapillata and is highly cytolytic against various human cells including erythrocyte.
Wei Yuen Yap +3 more
doaj +1 more source
Replacing batch unit operations of biopharmaceuticals by continuous manufacturing is a maturing concept, with periodic counter-current chromatography (PCC) favoured to replace batch chromatography.
João P. Mendes +5 more
doaj +1 more source

