Results 31 to 40 of about 2,190,614 (293)

Continuous Affinity Purification of Adeno-Associated Virus Using Periodic Counter-Current Chromatography

Pharmaceutics, 2022
Replacing batch unit operations of biopharmaceuticals by continuous manufacturing is a maturing concept, with periodic counter-current chromatography (PCC) favoured to replace batch chromatography.
João P. Mendes, Magnus Bergman, Anita Solbrand, Cristina Peixoto, Manuel J. T. Carrondo, Ricardo J. S. Silva   +5 more
doaj   +1 more source

Comparison of membrane affinity-based method with size-exclusion chromatography for isolation of exosome-like vesicles from human plasma

Journal of Translational Medicine, 2018
Plasma extracellular vesicles (EVs), especially exosome-like vesicles (ELVs), are being increasingly explored as a source of potential noninvasive disease biomarkers.
R. Stranska, Laurens Gysbrechts, Jens Wouters, P. Vermeersch, Katarzyna Bloch, D. Dierickx, G. Andrei, R. Snoeck   +7 more
semanticscholar   +1 more source

Produção, purificação e aumento da performance de ciclodextrina glicosiltransferases para produção de ciclodextrinas Production, purification and performance improve of cyclodextrin glycosyl transferases to cyclodextrins production

Química Nova, 2009
Biospecific affinity chromatography was used to purify three cyclodextrin glycosyl transferases (CGTases) obtained from microorganisms isolated of soil.
Cristiane Moriwaki, Cassiana Mazzer, Rúbia Pazzetto, Graciette Matioli   +3 more
doaj   +1 more source

Interaction of the 89K murine cytomegalovirus immediate-early protein with core histones [PDF]

, 1988
The conditions that permit the interaction of immediate-early proteins of murine cytornegalovirus (MCMV) with DNA were studied. Chromatography of extracts from infected cells on MCMV DNA cellulose and calf thymus DNA cellulose showed that pp89, the ...
Alberts, Bookout, Ebeling, Einck, Everett, Faber, Freeman, Green, Günther M. Keil, Hay, Hermiston, Isenberg, Kedes, Keil, Keil, Keil, Keil, Konrad Munch, Koszinowski, Koszinowski, Koszinowski, Kristie, Kristie, Martin Messerle, O'Hare, Ohlenbusch, Purifoy, Reddehase, Rigby, Stein, Tevethia, Ulrich H. Koszinowski, Weissbach   +32 more
core   +1 more source

Rapid determination of serum albumin partition coefficients using affinity chromatography

Environmental Advances, 2022
Sorption to serum albumin is essential for characterizing the distribution of chemicals in organisms. It impacts the concentration of the freely dissolved chemical in vivo and in vitro, and needs to be considered when effect concentrations at the target ...
Nadin Ulrich, Alexander Böhme
doaj   +1 more source

Minimal Information About Sample Preparation for Phosphoproteomics [PDF]

, 2009
This guideline describes parameters and conditions involved in phosphopeptide sample preparation. It covers from the description and preparation of the cells and tissues to the fractionation and specific enrichment of phosphopeptides for MS analysis. The
David Ovelleiro, Joaquin Abian, Marina Gay, Montserrat Carrascal   +3 more
core   +2 more sources

Novel ionization reagent for the measurement of gas-phase ammonia and amines using a stand-alone atmospheric pressure gas chromatography (APGC) source. [PDF]

, 2020
RationaleContaminants present in ambient air or in sampling lines can interfere with the target analysis through overlapping peaks or causing a high background. This study presents a positive outcome from the unexpected presence of N-methyl-2-pyrrolidone,
Finlayson-Pitts, Barbara J, Li, Xiaoxiao, Perraud, Véronique, Smith, James N   +3 more
core   +1 more source

Single-Step Purification of Monomeric l-Selectin via Aptamer Affinity Chromatography

Sensors, 2017
l-selectin is a transmembrane receptor expressed on the surface of white blood cells and responsible for the tethering of leukocytes to vascular endothelial cells.
Christian Kuehne, Stefanie Wedepohl, Jens Dernedde   +2 more
doaj   +1 more source

Cyclodextrin production by Bacillus lehensis isolated from cassava starch: Characterisation of a novel enzyme

Czech Journal of Food Sciences, 2014
The properties of a previously unknown enzyme, denominated cyclodextrin glycosyltransferase, produced from Bacillus lehensis, were evaluated using affinity chromatography for protein purification.
Kate Cristina Blanco, Flávio Faria De Moraes, Natalia Sozza Bernardi, Mary Helen Palmuti Braga Vettori, Rubens Monti, Jonas Contiero   +5 more
doaj   +1 more source

A general strategy for discovery of inhibitors and activators of RING and U-box E3 ligases with ubiquitin variants [PDF]

, 2017
RING and U-box E3 ubiquitin ligases regulate diverse eukaryotic processes and have been implicated in numerous diseases, but targeting these enzymes remains a major challenge.
Ahmed, Syed Feroj, Buetow, Lori, Gabrielsen, Mads, Huang, Danny T., Nakasone, Mark A., Sibbet, Gary J., Sidhu, Sachdev S., Smith, Brian O., Zhang, Wei   +8 more
core   +3 more sources