PVA-Glutaraldehyde as support for lectin immobilization and affinity chromatography
Acta Scientiarum: Biological Sciences, 2016 Immobilized lectins are a powerful biotechnological tool for separation and isolation of glycoconjugates. In the present study, polyvinyl alcohol (PVA) and glutaraldehyde (GA) were used as a support for Concanavalin A (Con A) covalent immobilization and ...
Moacyr Jesus Barreto de Melo Rêgo +5 more
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A Monomer-to-Trimer Transition of the Human Mitochondrial Transcription Termination Factor (mTERF) Is Associated with a Loss of in Vitro Activity [PDF]
, 2004 The human mitochondrial transcription termination factor (mTERF) is a nuclear-encoded 39-kDa protein that recognizes a mtDNA segment within the mitochondrial tRNALeu(UUR) gene immediately adjacent to and downstream of the 16 S rRNA gene. Binding of mTERF
Asin-Cayuela, Jordi +2 more
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Advance chromatin extraction improves capture performance of protein A affinity chromatography.
Journal of Chromatography A, 2016 Practical effects of advance chromatin removal on performance of protein A affinity chromatography were evaluated using a caprylic acid-allantoin-based extraction method.
Rui Nian +7 more
semanticscholar +1 more source
Journal of Chromatography A, 2016 Affinity chromatography is a convenient way of purifying proteins, as a high degree of purity can be reached in one step. The use of tags has greatly contributed to the popularity of this technique.
G. Béhar +3 more
semanticscholar +1 more source
Purification of phage display-modified bacteriophage T4 by affinity chromatography
BMC Biotechnology, 2011 Background Affinity chromatography is one of the most efficient protein purification strategies. This technique comprises a one-step procedure with a purification level in the order of several thousand-fold, adaptable for various proteins, differentiated
Figura Grzegorz +6 more
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Conformational plasticity of IgG during protein A affinity chromatography.
Journal of Chromatography A, 2016 Single step elution of a protein A column with 100mM acetate pH 3.5 produced a curvilinear gradient with pH dropping steeply at first then more gradually as it approached endpoint. IgG with a native hydrodynamic diameter of 11.5 nm began to elute at pH 6.
P. Gagnon, Rui Nian
semanticscholar +1 more source
Effect of Q Chromatography on the Recovery of Human Plasminogen in Affinity Chromatography
Zhongguo shuxue zazhi[Objective] To compare quality control (relative purity and specific activity) and process control [plasminogen (Pg) antigen recovery and potency recovery] indexes of samples before and after adding the Q chromatography step to the full chromatography ...
YUE Shenglan +12 more
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Endogenous mammalian lectin localized extracellularly in lung elastic fibers. [PDF]
, 1984 An affinity-purified antibody preparation raised against a beta-galactoside-binding lectin from bovine lung was used to localize a similar lectin in rat lung by immunofluorescence and by electron microscopy after on-grid staining visualized with ...
Barondes, SH +2 more
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Purification of rabbit serum histidine-proline-rich glycoprotein via preparative gel electrophoresis and characterization of its glycosylation patterns. [PDF]
PLoS ONE, 2017 Histidine-Proline-rich Glycoprotein (HPRG) is a plasma protein of vertebrates and several marine bivalves. Due to its multidomain structure consisting of several regions HPRG can interact with a variety of ligands, however the exact physiological role ...
Anna Katharina Weyrauch +4 more
doaj +1 more source
Surface Mutation Thr34His Facilitates Purification of Haemophilus influenza Carbonic Anhydrase via Metal Affinity Chromatography [PDF]
, 2017 In order to pursue Haemophilus influenza carbonic anhydrase (HICA) as a potential drug target, easy and efficient purification methods must be developed. While immobilized metal affinity chromatography (IMAC) may be used, complications with polyhistidine
Cornely, Dr. Kathleen, Foley, Caroline
core +1 more source