Results 21 to 30 of about 2,190,614 (293)

Dimerization capacities of FGF2 purified with or without heparin-affinity chromatography. [PDF]

open access: yesPLoS ONE, 2014
Fibroblast growth factor-2 (FGF2) is a pleiotropic growth factor exhibiting a variety of biological activities. In this article, we studied the capacity of FGF2 purified with or without heparin affinity chromatography to self-associate. Analyzing the NMR
Natalia Platonova   +8 more
doaj   +1 more source

Separation of virus-like particles and extracellular vesicles by flow-through and heparin affinity chromatography.

open access: yesJournal of Chromatography A, 2019
Separation of enveloped virus-like particles from other extracellular vesicles is a challenging separation problem due to the similarity of these bionanoparticles.
Katrin Reiter   +5 more
semanticscholar   +1 more source

Effects of antibodies against tubulin on the movement of reactivated sea urchin sperm flagella [PDF]

open access: yes, 1980
Antibodies binding to sea urchin flagellar outer-doublet tubulin have been isolated from rabbit sera by tubulin-affinity chromatography employing electrophoretically purified tubulin as the immobilized substrate.
Asai, David J., Brokaw, Charles J.
core   +1 more source

The use of phenyl-Sepharose for the affinity purification of proteinases [PDF]

open access: yes, 1993
Phenyl-Sepharose is most often used as an adsorbent for hydrophobic interaction chromatography (HIC). We report on its effective use for the affinity purification of some extracellular thermostable proteinases from bacterial sources.
Daniel, Roy M.   +3 more
core   +2 more sources

Affinity Chromatography: An Enabling Technology for Large‐Scale Bioprocessing

open access: yesBiotechnology Journal, 2019
Affinity chromatography (AC) has been used in large‐scale bioprocessing for almost 40 years and is considered the preferred method for primary capture in downstream processing of various types of biopharmaceuticals.
K. Łącki, F. Riske
semanticscholar   +1 more source

Purification of baculovirus vectors using heparin affinity chromatography

open access: yesMolecular Therapy: Methods & Clinical Development, 2016
Baculoviruses are commonly used for recombinant protein and vaccine production. Baculoviruses are nonpathogenic to vertebrates, have a large packaging capacity, display broad host and cell type tropism, infect both dividing and nondividing cells, and do ...
Md Nasimuzzaman   +3 more
doaj   +1 more source

Enhanced production of recombinant HALT-1 pore-forming toxin using two-step chromatographic procedure

open access: yesMethodsX, 2023
Hydra actinoporin-like toxin-1 (HALT-1) has been isolated from Hydra magnipapillata and is highly cytolytic against various human cells including erythrocyte.
Wei Yuen Yap   +3 more
doaj   +1 more source

Non-targeted characterization of attributes affecting antibody-FcγRIIIa V158 (CD16a) binding via online affinity chromatography-mass spectrometry

open access: yesmAbs, 2022
Antibodies facilitate targeted cell killing by engaging with immune cells such as natural killer cells through weak binding interactions with Fcγ receptors on the cell surface.
Daniel W. Woodall   +6 more
doaj   +1 more source

Matrices and Affinity Ligands for Antibody Purification and Corresponding Applications in Radiotherapy

open access: yesBiomolecules, 2022
Antibodies have become an important class of biological products in cancer treatments such as radiotherapy. The growing therapeutic applications have driven a demand for high-purity antibodies. Affinity chromatography with a high affinity and specificity
Aiying Xue, Saijun Fan
doaj   +1 more source

Molecular mechanisms of the non-coenzyme action of thiamin in brain. Biochemical, structural and pathway analysis [PDF]

open access: yes, 2015
Thiamin (vitamin B1) is a pharmacological agent boosting central metabolism through the action of the coenzyme thiamin diphosphate (ThDP). However, positive effects, including improved cognition, of high thiamin doses in neurodegeneration may be ...
Andrey, Vovk   +9 more
core   +1 more source

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