Results 21 to 30 of about 1,195,639 (276)

Produção, purificação e aumento da performance de ciclodextrina glicosiltransferases para produção de ciclodextrinas Production, purification and performance improve of cyclodextrin glycosyl transferases to cyclodextrins production

open access: yesQuímica Nova, 2009
Biospecific affinity chromatography was used to purify three cyclodextrin glycosyl transferases (CGTases) obtained from microorganisms isolated of soil.
Cristiane Moriwaki   +3 more
doaj   +1 more source

Rapid determination of serum albumin partition coefficients using affinity chromatography

open access: yesEnvironmental Advances, 2022
Sorption to serum albumin is essential for characterizing the distribution of chemicals in organisms. It impacts the concentration of the freely dissolved chemical in vivo and in vitro, and needs to be considered when effect concentrations at the target ...
Nadin Ulrich, Alexander Böhme
doaj   +1 more source

Matrices and Affinity Ligands for Antibody Purification and Corresponding Applications in Radiotherapy

open access: yesBiomolecules, 2022
Antibodies have become an important class of biological products in cancer treatments such as radiotherapy. The growing therapeutic applications have driven a demand for high-purity antibodies. Affinity chromatography with a high affinity and specificity
Aiying Xue, Saijun Fan
doaj   +1 more source

Single-Step Purification of Monomeric l-Selectin via Aptamer Affinity Chromatography

open access: yesSensors, 2017
l-selectin is a transmembrane receptor expressed on the surface of white blood cells and responsible for the tethering of leukocytes to vascular endothelial cells.
Christian Kuehne   +2 more
doaj   +1 more source

Cyclodextrin production by Bacillus lehensis isolated from cassava starch: Characterisation of a novel enzyme

open access: yesCzech Journal of Food Sciences, 2014
The properties of a previously unknown enzyme, denominated cyclodextrin glycosyltransferase, produced from Bacillus lehensis, were evaluated using affinity chromatography for protein purification.
Kate Cristina Blanco   +5 more
doaj   +1 more source

High-grade extracellular vesicles preparation by combined size-exclusion and affinity chromatography

open access: yesScientific Reports, 2021
Extracellular vesicles (EVs) have recently gained growing interest for their diagnostic and therapeutic potential. Despite this, few protocols have been reported for the isolation of EVs with preserved biological function.
Cristina Bellotti   +4 more
doaj   +1 more source

Cryogels with Affinity Ligands as Tools in Protein Purification

open access: yes, 2015
Affinity chromatography is one of the well-known separation techniques especially if high purity is desired. Introducing ligands on monolithic structure gives the possibility for purifying complex media such as plasma and crude extract.
Bo Mattiasson   +4 more
core   +1 more source

PVA-Glutaraldehyde as support for lectin immobilization and affinity chromatography

open access: yesActa Scientiarum: Biological Sciences, 2016
Immobilized lectins are a powerful biotechnological tool for separation and isolation of glycoconjugates. In the present study, polyvinyl alcohol (PVA) and glutaraldehyde (GA) were used as a support for Concanavalin A (Con A) covalent immobilization and ...
Moacyr Jesus Barreto de Melo Rêgo   +5 more
doaj   +1 more source

Purification of phage display-modified bacteriophage T4 by affinity chromatography

open access: yesBMC Biotechnology, 2011
Background Affinity chromatography is one of the most efficient protein purification strategies. This technique comprises a one-step procedure with a purification level in the order of several thousand-fold, adaptable for various proteins, differentiated
Figura Grzegorz   +6 more
doaj   +1 more source

Effect of Q Chromatography on the Recovery of Human Plasminogen in Affinity Chromatography

open access: yesZhongguo shuxue zazhi
[Objective] To compare quality control (relative purity and specific activity) and process control [plasminogen (Pg) antigen recovery and potency recovery] indexes of samples before and after adding the Q chromatography step to the full chromatography ...
YUE Shenglan   +12 more
doaj   +1 more source

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