Results 51 to 60 of about 308,685 (162)

Reaction of Native and Denatured Brucella abortus (S19) Proteins with Antibody Using Affinity Chromatography and Immunoblotting

پزشکی بالینی ابن سینا, 2005
Western blotting or immunoblotting commonly use for study of reaction between antigens and antibodies. Denaturation of many proteins in immunoblotting can affect greatly the reactivity of antibodies and outcome of the procedure.
Ramin Karimi, Ali Mostafaie, Bahman Tabaraie, Yadollah Bahrami, Jalal Abdolalizadeh   +4 more
doaj  

Feasibility of preparing human plasminogen by full chromatography from precipitation of Fraction Ⅲ in low temperature ethanol method

Zhongguo shuxue zazhi
[Objective] To determine the feasibility of preparing plasminogen (Pg) with Fraction Ⅲ precipitation (hereinafter referred to as FⅢ-P) from low-temperature ethanol process by full chromatography (hereinafter referred to as FⅢ-P process). [Methods] The FⅢ-
ZHANG Jin, YUE Shenglan, ZHU Chen, PENG Yan, ZHOU Yanxiang, LIN Lianzhen, CHEN Kejin, FENG Lu, HU Yong, ZHOU Zhijun   +9 more
doaj   +1 more source

A New Affinity Gel Synthesized for Phenylalanine Ammonia Lyase Isolated from Red Clover (Trifolium pratense L.) Leaf and an Investigation into Its Kinetic Properties

Separations
Phenylalanine ammonia lyase (PAL) was first purified using affinity chromatography from the leaves of red-flowered clover, a highly antioxidant source.
Yavuz Selim Toksöz, Çiğdem Bilen, Emine Karakuş   +2 more
doaj   +1 more source

Evaluation of Caesalpinia pulcherrima endospermic gum as affinity matrices for galactose-binding lectins interaction

Brazilian Archives of Biology and Technology, 2011
Lectins are proteins or glycoproteins able to bind, specifically and reversibly carbohydrates and glycoconjugates. Considering this ability, the utilization of Caesalpinia pulcherrima seeds polysaccharides as an affinity matrix was tested.
Renata Chastinet Braga, Daniele Maria Alves Teixeira-Sá, Alexsandra Feitosa Ribeiro, Raquel Lima Miranda, Lia Magalhães de Almeida, Ana Cecília Góes Horta, Renato de Azevedo Moreira   +6 more
doaj   +1 more source

Characterization of the galactomannans from Parkinsonia aculeata seeds and their application on affinity chromatography

Polímeros, 2006
Successive aqueous (10 ºC, 25 ºC and 80 ºC) and alkaline (1M NaOH; 25 ºC) extractions of milled Parkinsonia aculeata endosperms gave rise to four galactomannan fractions. These extractions furnished viscous galactomannans with Man:Gal ratios ranging from
Ivone Garros-Rosa, Fany Reicher, Carmen L. O. Petkowicz, Maria Rita Sierakowski, Renato A. Moreira   +4 more
doaj   +1 more source

Solid-Supported Proteins in the Liquid Chromatography Domain to Probe Ligand-Target Interactions

Frontiers in Chemistry, 2019
Ligand-target interactions play a central role in drug discovery processes because these interactions are crucial in biological systems. Small molecules-proteins interactions can regulate and modulate protein function and activity through conformational ...
Marcela Cristina de Moraes, Carmen Lucia Cardoso, Quezia Bezerra Cass   +2 more
doaj   +1 more source

A streamlined tandem affinity purification of His-MBP-SpyCas9, without buffer exchange, suitable for in vitro cleavage applications

MethodsX
We present a streamlined protocol for the purification of recombinant doubly-tagged His-MBP-SpyCas9 protein utilizing a dual affinity chromatography approach whereby the elution volume from immobilized metal ion affinity chromatography (IMAC) is loaded ...
Filippo Fronza, Roberto Verardo, Claudio Schneider   +2 more
doaj   +1 more source

Developing a process of lentivirus purification from recombinant fluids using peptide affinity ligands

Bioengineering & Translational Medicine
Lentiviral vectors (LVVs) are emerging as an enabling tool in gene and cell therapies, yet the toolkit for purifying them at scale is still immature. A pivoting moment in LVV isolation technology was marked by the introduction of affinity ligands for ...
Eduardo Barbieri, Gina N. Mollica, Sobhana A. Sripada, Shrirarjun Shastry, Yuxuan Wu, Arianna Minzoni, Will Smith, Elena Wuestenhagen, Annika Aldinger, Heiner Graalfs, Michael S. Crapanzano, Oliver Rammo, Michael M. Schulte, Michael A. Daniele, Stefano Menegatti   +14 more
doaj   +1 more source

Polyclonal Antibody Production against Mouse Purified IgG2a towards Use in Basic Research

Research in Molecular Medicine, 2016
Background: The ability of polyclonal antibodies to react with many epitopes of an antigen makes them valuable reagents in research and diagnosis.
Zahra Moradi nebrin, Jafar Majidi, Leili Aghebati Maleki, Tohid Kazemi, Jalal Abdolalizadeh, Somaieh Dadashi, Sadeg Eyvazi, Majid Ahmadi, Naiemeh Majidi zolbanin   +8 more
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PURIFICATION AND ANALYSIS OF CARBOHYDRATE CLUSTER FROM ERYTHROPOIETIN RECOMBINANT EXPRESSION RESULT ON THE LEAVEN SYSTEM OF Pichia pastoris

Makara Seri Sains, 2011
For clinical purposes, pure protein and identification of carbohydrate structure from erythropoietin recombinant are needed. Purification was done with His-Trap affinity chromatography method and continued with gel filtration chromatography column to get
Adi Santoso, Andri Wardiana
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