Results 81 to 90 of about 29,610 (215)

Ion exchange chromatography – basic principles and application [PDF]

open access: yes, 2011
Ion-Exchange Chromatography (IEC) allows for the separation of ionizable molecules on the basis of differences in charge properties. Its large sample-handling capacity, broad applicability (particularly to proteins and enzymes), moderate cost, powerful ...
Cummins, Phil   +2 more
core  

Purification, Characterization and Antioxidant Activities <em>in Vitro</em><em> </em>and <em>in Vivo</em> of the Polysaccharides from <em>Boletus edulis</em> Bull

open access: yesMolecules, 2012
A water-soluble polysaccharide (BEBP) was extracted from <em>Boletus edulis </em>Bull using hot water extraction followed by ethanol precipitation.
Yijun Fan   +3 more
doaj   +1 more source

Purification of algnic acid by Local isolate of Azotobacter Vinelandi

open access: yesIbn Al-Haitham Journal for Pure and Applied Sciences, 2017
The bacteria Azotobacter Vinelandii  was taken from a central research in Baghdad, The purification of alginic acid which produced from the bacteria by several steps starting with precipitation with isopropanol (3:1) v/v , Washing by ppt with 100ml of ...
J.I. Mahdi, M.R. Ali, H.M. Hamza
doaj  

Isolation, Purification, Fractionation, and Hepatoprotective Activity of Polygonatum Polysaccharides

open access: yesMolecules
In this study, three homogeneous fractions, PSP-N-b-1, PSP-N-b-2, and PSP-N-c-1, were obtained from an aqueous extract of Polygonatum using DEAE cellulose column chromatography, CL-6B agarose gel chromatography, and Sephadex G100 chromatography.
Yutong Wang   +3 more
doaj   +1 more source

Purification of phospholipase B from Penicillium notatum by hydrophobic chromatography on palmitoyl cellulose.

open access: yesJournal of Lipid Research, 1980
Phospholipase B (lysolecithin acyl-hydrolase, EC 3.1.1.5) from the mycelia of Penicillium notatum (Institute for Fermentation, Osaka, Japan; No.4640) was adsorbed from a crude solution to palmitoyl cellulose. Adsorption was efficient at pH 4 at low ionic
S Imamura, Y Horiuti
doaj   +1 more source

Purification and characterization of alginate lyase from locally isolated marine Pseudomonas stutzeri MSEA04 [PDF]

open access: yes, 2016
An alginate lyase with high specific enzyme activity was purified from Pseudomonas stutzeri MSEA04, isolated from marine brown algae. The alginate lyase was purified by precipitation with ammonium sulphate, acetone and ethanol individually.
Beltagy, Ehab A.   +3 more
core   +1 more source

Measurement and purification of Alanine aminotransferase (ALT) enzyme activity in patients with celiac disease

open access: yesمجلة بغداد للعلوم, 2017
Celiac disease (CD) is the most common genetically - based disease in correlation with food intolerance. The aim of this study is to measure the activity of ALT enzyme and purify enzyme from sera women with celiac disease.
Taghreed U. Mohammed   +2 more
doaj   +1 more source

Electron microscopic visualization of tRNA genes with ferritin-avidin: biotin labels [PDF]

open access: yes, 1978
A method is described for indirect electron microscopic visualization and mapping of tRNA and other short transcripts hybridized to DNA. This method depends upon the attachment of the electron-dense protein ferritin to the RNA, the binding being mediated
Angerer, Lynne M.   +4 more
core   +2 more sources

Cloned mouse cells with natural killer function and cloned suppressor T cells express ultrastructural and biochemical features not shared by cloned inducer T cells. [PDF]

open access: yes, 1983
We have examined the morphology, cytochemistry, and biochemistry of mouse leukocyte subsets by analyzing cloned leukocyte populations specialized to perform different immunologic functions.
Cantor, H   +10 more
core  

PURIFICATION OF PEROXIDASE-LABELED ANTIBODY BY USING DEAE-CELLULOSE CHROMATOGRAPHY

open access: yesACTA HISTOCHEMICA ET CYTOCHEMICA, 1976
A method which facilitates the separation of the conjugated γ-globulin from both non-labeled antibody and unreacted peroxidase by use of DEAE-cellulose chromatography was shown in this paper. Using the new method of conjugation reported by Nakane and Kawaoi (1974), the isoelectric point of the peroxidase-labeled antibody was found appreciably to shift ...
S. YAMASHITA, N. YAMAMOTO, K. YASUDA
openaire   +2 more sources

Home - About - Disclaimer - Privacy