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Reproducibility of gel‐chromatography

International Journal of Peptide and Protein Research, 1984
We think it worthwhile to other experimenters to mention the following observations. Recently it was noted that gel chromatographic separations of the five fragments resulting from the degradation of cytochrome c by bromocyanogen became dependent on the batch number of the gel. This dependency concerns only separations of unprotected fragments, using 7%
Tesser, G.I.   +3 more
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Gel chromatography of heparin

Journal of Chromatography B: Biomedical Sciences and Applications, 1981
It is assumed that heparin is a heterogeneous substance. In order to further investigate the purification of heparin, a column chromatographic technique for the fractionation of heparin is described using various diameters of bead form cross-linked dextran gels and an automated apparatus. It was observed that Sephadex G-50 resulted in the separation of
R, Losito, H, Gattiker, G, Bilodeau
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Gel‐Filtration Chromatography

Current Protocols in Molecular Biology, 1998
AbstractGel filtration (GF) chromatography separates proteins solely on the basis of molecular size. Separation is achieved using a porous matrix to which the molecules, for steric reasons, have different degrees of access‐‐i.e., smaller molecules have greater access and larger molecules are excluded from the matrix. Hence, proteins are eluted from the
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Gel matrices for scanning gel chromatography

Biophysical Chemistry, 1979
I have examined the light-scattering behavior of a number of gel matrices used in gel filtration chromatography. The angular dependence of light scattering by Sephadexes is consistent with treatment of the particles as large scattering particles with a low refractive index increment (mu).
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Gel Chromatography

Angewandte Chemie International Edition in English, 1970
AbstractGel chromatography can be regarded as a network‐limited partitioning of substances. The theory of this method and the preparation and properties of various gel systems are reviewed. Optimization of the method is illustrated for the separation of oligomers.
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Gel Filtration Chromatography

Separation Science, 1971
Abstract The technique of gel filtration chromatography is described, with a guide to relevant literature on methods, theory, and applications, especially the use of GFC in analysis of molecular interactions, both macromolecular and small molecule-macromolecule aggregation.
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Exclusion Chromatography Gel Permeation Chromatography

1979
In contrast to the separation techniques discussed up to now, separation by exclusion chromatography involves a single, unambiguous mechanism. In the absence of interactions (by definition) between the sample and the stationary phase surface, the elution order (or elution volume) is solely a function of the molecular size (see IX.B). Hence, this method
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Gel permeation chromatography

Pigment & Resin Technology, 1977
Resins used in surface coatings are mostly polymers of one sort or another and although they differ in a number of ways from the high polymers used to make plastics and synthetic fibres, they nevertheless show some of those properties peculiar to polymers.
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Gel permeation chromatography

Journal of Chromatography A, 1976
Abstract An automated gel permeation chromatograph-low-angle laser light-scattering system capable of measuring the molecular-weight distribution of soluble polymers without the necessity of calibration is described. The automation system eliminates most of the tediousness associated with molecular-weight distribution measurement.
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