Results 51 to 60 of about 729,544 (290)

Purification of rat pancreatic lipase

Journal of Lipid Research, 1968
A procedure for the isolation of lipase (glycerolester hydrolase, EC 3.1.1.3) from rat pancreas is described. The purification scheme includes homogenization of the pancreas. centrifugation at 3,000 rpm, centrifugation at 40,000 rpm.
Lewis I. Gidez
doaj   +1 more source

Isolation and characterisation of metallothionein from the clam Ruditapes decussatus [PDF]

, 2003
Metallothioneins (MT) were obtained after purification from metal-exposed clams (Ruditapes decussatus) using gel-permeation and ion-exchange chromatography.
Bebianno, Bebianno, Bebianno, Berger, Bernhard, Blum, Braun, Brouwer, Chan, Chubatsu, Coleman, Dabrio, Dallinger, Hammer, Kagi, Kagi, Kagi, Kagi, Kagi, Khoo, Kille, Langston, Lin, Lipman, Mackay, Mitra, Narula, Nordberg, Olafson, Pedersen, Raspor, Riek, Robbins, Roesijadi, Roesijadi, Schmidt, Unger, Vasak, Vasak, Wang, Winge, Zeng, Zeng   +42 more
core   +2 more sources

Production of antibacterial compounds by a Steely hybrid polyketide synthase in Dictyostelium

FEBS Open Bio, EarlyView.
By selecting appropriate production species under optimised culture conditions, new chlorinated dibenzofuran compounds, which previously could not be analysed in sufficient quantities, were identified from cellular slime mould fruiting bodies. The strong antibacterial activity exhibited by these compounds suggests a link to the ecological function of ...
Tomoaki R. Yamashita, Toyonobu Usuki, Robert R. Kay, Tamao Saito   +3 more
wiley   +1 more source

Gel permeation chromatography process for highly oriented Cs3Cu2I5 nanocrystal film

Scientific Reports, 2022
The emergence of green materials has attracted considerable attention in the field of optoelectronics. Copper-based lead-free metal halide (with a near-unity quantum yield) obtained from Cs3Cu2I5 nanocrystals (NCs) can exhibit blue emission with a ...
Yu-Hong Cheng, Rikuo Suzuki, Narumi Shinotsuka, Hinako Ebe, Naoaki Oshita, Ryohei Yamakado, Takayuki Chiba, Akito Masuhara, Junji Kido   +8 more
doaj   +1 more source

Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein

FEBS Open Bio, EarlyView.
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley   +1 more source

Stereoisomer libraries: Total synthesis of all 16 stereoisomers of the pine sawfly sex pheromone by a fluorous mixture-synthesis approach [PDF]

, 2004
All 16 stereoisomers of the sex pheromone of pine sawfly (3,7,11-trimethylundecanol propanoate ester) have been synthesized on a 10- to 20-mg scale by a split-parallel fluorous mixture-synthesis approach.
Curran, D. P. Curran, Hedenstr  m, Houghten, Luo, M. Jeske, S. Dandapani, Zhang, Zhang, Zhang   +9 more
core   +3 more sources

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Purification of a 19-kDa pore-forming cytolysin from the sea anemone Heteractis magnifica

Journal of Venomous Animals and Toxins including Tropical Diseases, 2010
Pore-forming cytolysins of 19 kDa from sea anemones present a remarkable cytolytic property. In the present work, a purified 19-kDa cytolysin was obtained from the sea anemone Heteractis magnifica.
S Karthikayalu, V Rama, R Venkatesan
doaj   +1 more source

ATG4B is required for mTORC1‐mediated anabolic activity and is associated with clinical outcomes in non‐small cell lung cancer

FEBS Open Bio, EarlyView.
The relationship between anabolic and catabolic processes governing lung cancer cell growth is nuanced. We show that ATG4B, an autophagy regulator, is elevated in lung cancer and that high ATG4B is associated with worse patient outcomes. Targeting ATG4B in cells reduces growth, protein synthesis, and mTORC1 activity, demonstrating a new relationship ...
Patrick J. Ryan, Bethany C. Guerra, Selina Uranga, Jessica M. Cardin, Steven E. Riechman, Mariana Janini Gomes, James D. Fluckey   +6 more
wiley   +1 more source

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source