Results 51 to 60 of about 1,439,714 (304)

VGF C-terminus fragments found by gel chromatography in human.

open access: yes, 2016
VGF C-terminus fragments found by gel chromatography in human.
Roberta Puddu (3219888)   +15 more
core   +1 more source

Purification of rat pancreatic lipase

open access: yesJournal of Lipid Research, 1968
A procedure for the isolation of lipase (glycerolester hydrolase, EC 3.1.1.3) from rat pancreas is described. The purification scheme includes homogenization of the pancreas. centrifugation at 3,000 rpm, centrifugation at 40,000 rpm.
Lewis I. Gidez
doaj   +1 more source

Structural and biochemical analysis of a B12 superbinder

open access: yesFEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices   +3 more
wiley   +1 more source

Gel permeation chromatography process for highly oriented Cs3Cu2I5 nanocrystal film

open access: yesScientific Reports, 2022
The emergence of green materials has attracted considerable attention in the field of optoelectronics. Copper-based lead-free metal halide (with a near-unity quantum yield) obtained from Cs3Cu2I5 nanocrystals (NCs) can exhibit blue emission with a ...
Yu-Hong Cheng   +8 more
doaj   +1 more source

Structures of mycobacterial 3‐methylcrotonyl‐CoA carboxylase reveal carrier‐domain translocation between catalytic sites

open access: yesFEBS Letters, EarlyView.
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav   +2 more
wiley   +1 more source

Analytical gel filtration chromatography.

open access: yes, 2016
Analytical gel filtration chromatography of BcGR was performed in the absence of substrates (solid line) and in the presence of 10 mM D-Glu (dashed line) or L-glutamate (dotted line). The inset represents the molecular weight calibration curve, performed
Silvia Buroni (65276)   +6 more
core   +1 more source

Purification of a 19-kDa pore-forming cytolysin from the sea anemone Heteractis magnifica

open access: yesJournal of Venomous Animals and Toxins including Tropical Diseases, 2010
Pore-forming cytolysins of 19 kDa from sea anemones present a remarkable cytolytic property. In the present work, a purified 19-kDa cytolysin was obtained from the sea anemone Heteractis magnifica.
S Karthikayalu, V Rama, R Venkatesan
doaj   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

open access: yesFEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge   +6 more
wiley   +1 more source

Test No. 119: Determination of the Low Molecular Weight Content of a Polymer Using Gel Permeation Chromatography [electronic resource] /

open access: yes, 1996
This Test guideline describes the Gel Permeation Chromatography (GPC). This method permits to determine the molecular weight distribution and the average molecular weights (Mn, Mw).
Organisation for Economic Co-operation and Development.   +1 more
core   +1 more source

Purification and partial characterization of superoxide dismutase from the thermophilic bacteria Thermothrix sp. [PDF]

open access: yesJournal of the Serbian Chemical Society, 2004
Superoxide dismutase (SOD; EC 1.15.1.1.), a high molecular weight component of the antioxidant defense system, provided promising results in the treatment of oxidative damage.
RATKO M. JANKOV   +3 more
doaj   +3 more sources

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