Results 51 to 60 of about 1,439,714 (304)
VGF C-terminus fragments found by gel chromatography in human.
VGF C-terminus fragments found by gel chromatography in human.
Roberta Puddu (3219888) +15 more
core +1 more source
Purification of rat pancreatic lipase
A procedure for the isolation of lipase (glycerolester hydrolase, EC 3.1.1.3) from rat pancreas is described. The purification scheme includes homogenization of the pancreas. centrifugation at 3,000 rpm, centrifugation at 40,000 rpm.
Lewis I. Gidez
doaj +1 more source
Structural and biochemical analysis of a B12 superbinder
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices +3 more
wiley +1 more source
Gel permeation chromatography process for highly oriented Cs3Cu2I5 nanocrystal film
The emergence of green materials has attracted considerable attention in the field of optoelectronics. Copper-based lead-free metal halide (with a near-unity quantum yield) obtained from Cs3Cu2I5 nanocrystals (NCs) can exhibit blue emission with a ...
Yu-Hong Cheng +8 more
doaj +1 more source
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav +2 more
wiley +1 more source
Analytical gel filtration chromatography.
Analytical gel filtration chromatography of BcGR was performed in the absence of substrates (solid line) and in the presence of 10 mM D-Glu (dashed line) or L-glutamate (dotted line). The inset represents the molecular weight calibration curve, performed
Silvia Buroni (65276) +6 more
core +1 more source
Purification of a 19-kDa pore-forming cytolysin from the sea anemone Heteractis magnifica
Pore-forming cytolysins of 19 kDa from sea anemones present a remarkable cytolytic property. In the present work, a purified 19-kDa cytolysin was obtained from the sea anemone Heteractis magnifica.
S Karthikayalu, V Rama, R Venkatesan
doaj +1 more source
The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge +6 more
wiley +1 more source
This Test guideline describes the Gel Permeation Chromatography (GPC). This method permits to determine the molecular weight distribution and the average molecular weights (Mn, Mw).
Organisation for Economic Co-operation and Development. +1 more
core +1 more source
Purification and partial characterization of superoxide dismutase from the thermophilic bacteria Thermothrix sp. [PDF]
Superoxide dismutase (SOD; EC 1.15.1.1.), a high molecular weight component of the antioxidant defense system, provided promising results in the treatment of oxidative damage.
RATKO M. JANKOV +3 more
doaj +3 more sources

