Results 81 to 90 of about 1,439,714 (304)
Dataset of gene cloning and gel filtration chromatography of R-est6
The data presented in this article are connected to the research article entitled “Expression, purification and biochemical characterization of a family 6 carboxylesterase from Methylococcus capsulatus (bath)” (Soni et al., 2016 [1]).
Surabhi Soni +3 more
doaj +1 more source
Application of gel chromatography [PDF]
Abstract
openaire +1 more source
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey +4 more
wiley +1 more source
The use of immunoglobulin Y (IgY) instead of polyclonal antibodies prepared in mammals has been reported recently. These antibodies are found to have better properties in terms of specificity and ease of large scale production.
M. Pour-Amir +3 more
doaj
Chromatography of lipids on commercial silica gel loaded filter paper
Commercial silica gel loaded filter paper has been found to give good separations of neutral lipids and phospholipids. Unidimensional or two-dimensional chromatography can be used. Rat liver lipids and C14-algae lipids were studied.
G.V. Marinetti
doaj +1 more source
A minimal cellulosome‐like system in Cellulosilyticum lentocellum
Cellulose‐degrading bacteria typically use cellulosomes, large multi‐enzyme complexes on a scaffold protein. In Cellulosilyticum lentocellum, we characterise a far smaller arrangement, a single scaffold bound to one cellulase through a single cohesin‐dockerin interaction.
John Allan +2 more
wiley +1 more source
Gel-filtration chromatographic analysis.
Gel-filtration chromatography was carried out by the AKTA-FPLC system. The column was calibrated with proteins of known molecular weight: thyroglobulin (670 kDa), γ-globulin (158 kDa), ovalbumin (44 kDa), and myoglobin (17 kDa).
Yen-Hua Huang (409275) +3 more
core +1 more source
Characterization of the key membrane protein cytochrome Cyc2 from Ghiorsea bivora
This study reports the first detailed biochemical analysis of Cyc2TAG1_1, a cytochrome protein involved in microbial iron oxidation. We produced and purified this enzyme in E. coli, then measured its iron‐oxidizing efficiency, and characterized its electrical and structural properties.
Josiane Azizi +6 more
wiley +1 more source
A wide range screening method for multiresidue analysis of seventy-seven pesticides (twelve organohalogens, forty-five organonitrogens, eleven organophosphorus and nine pyrethroids) in agricultural products is proposed.
Gelsomino A +4 more
core +1 more source
Extraction and purification of Ferritin from liver tissue
Background and Objective: Ferritin with molecular weight of 450 kDa is the most important iron storage protein and is made of 24 subunits consisting of light and heavy chains. Each Ferritin molecule is able to store 4500 Fe3+ molecules.
R Behjati Ardakani +4 more
doaj

