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High-pressure liquid chromatography of steroids

Journal of Chromatography A, 1979
After a brief discussion of the merits and limitations of high-pressure liquid chromatography (HPLC) relative to other chromatographic methods, special problems in the application to steroids are discussed. Publications on HPLC of steroids are then discussed under the headings of individual classes, arranged generally in the order of increasing ...
E, Heftmann, I R, Hunter
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High-pressure liquid chromatography of peptides

Journal of Chromatography A, 1977
Peptides varying in size from di- to decapeptide have been subjected to high-pressure liquid chromatography on Phenyl-Corasil, Poragel PN, and Poragel PS under reversed-phase conditions with acetonitrile-water mixtures. It has been found that residual silanol groups in the Phenyl-Corasil and the functional groups in the Poragels significantly influence
J J, Hansen   +4 more
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Thimerosal Determination by High-Pressure Liquid Chromatography

Journal of Pharmaceutical Sciences, 1978
A sensitive and useful high-pressure liquid chromatographic method for the determination of intact thimerosal was developed. This method is extremely fast and reliable, and its inherent specificity makes it a breakthrough over other common wet chemical methods.
R C, Meyer, L B, Cohn
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Analysis of leukotrienes by high-pressure liquid chromatography

Analytical Biochemistry, 1981
Abstract A reversed-phase high-pressure liquid chromatographic procedure has been developed to separate and quantitate leukotrienes. This procedure is based on the ultraviolet absorption of the conjugated triene moiety of these compounds and is sensitive to the nanogram level.
W R, Mathews, J, Rokach, R C, Murphy
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Laboratory Automation of High‐Pressure Liquid Chromatography

Journal of Pharmaceutical Sciences, 1975
An automated system for high-pressure liquid chromatography was developed. The system is built around commercial modules wherever possible, modified to varying degrees. An automatic sampler, a sample pump, a high-pressure sampling valve, a recorder with an integrator, and a high-pressure liquid chromatograph comprise the commercial instruments. Relays,
W F, Beyer, D D, Gleason
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Determination of hydroxyproline by high pressure liquid chromatography

Analytical Biochemistry, 1992
A rapid, precise, and simple HPLC method provides an assay of hydroxyproline from tissue extracts or solutions of collagen. Samples are hydrolyzed with 6 N HCl, derivatized with phenyl isothiocyanate, and chromatographed on a small, C18 reverse-phase HPLC column. Hydroxyproline (Hyp) is separated from other amino acids and detected by absorption at 254
G D, Green, K, Reagan
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High pressure liquid chromatography of neutral glycosphingolipids

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1972
Abstract A system for the separation of neutral glycosphingolipids by high pressure liquid chromatography of their benzoylated derivatives has been devised. Serum mono-, di-, tri-and tetraglycosylceramides are completely resolved within 25 min. A full scale recorder response can be obtained with approximately 1.0 nmole of glycolipid.
J E, Evans, R H, McCluer
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Analysis of Trisulfapyrimidines by High-Pressure Liquid Chromatography

Journal of Pharmaceutical Sciences, 1973
Abstract: The application of high-pressure liquid chromatography to the separation and analysis of trisulfapyrimidines in pharmaceutical dosage forms is demonstrated. The preparation of samples of both tablet and suspension dosage forms is simple and rapid.
R B, Poet, H H, Pu
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Anthracycline Assay by High-pressure Liquid Chromatography

Journal of Pharmaceutical Sciences, 1981
A general method of analysis of anthracycline concentrations was developed. Drug is extracted from plasma with organic solvent and separated from metabolites by high-pressure liquid chromatography on an aminocyanosilica column. Detection and quantitation are by the endogenous fluorescence of compounds having an intact tetracyclic ring structure. Limits
S D, Averbuch   +3 more
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Assay of Cefotaxime by High-Pressure-Liquid Chromatography

Chemotherapy, 2009
A high-pressure-liquid chromatographic (HPLC) procedure for quantitative assay of cefotaxime (CT) and its major metabolite in serum of normal individuals, desacetyl cefotaxime (DACT), is described. It employs Lichrosorb RP-8, elution with phosphoric-acid-methanol and UV absorption at 310 nm.
T, Bergan, R, Solberg
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