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Adsorption, Chromatography, Ion Exchange
2011Adsorption is the loading of solid surfaces with substances present in a surrounding fluid phase or, in other words, it is a surface effect between a solid and a fluid phase. Sometimes molecules of the fluid phase are not only fixed on the surface but can additionally enter the bulk of the nonporous solid phase according to a volume effect.
Alfons Mersmann +2 more
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Current Protocols in Protein Science, 1998
AbstractThis unit outlines the basic steps in planning and carrying out ion‐exchange chromatography to separate proteins. Protocols describe both batch adsorption and column chromatography in conjunction with either step‐ or linear elution gradients. Support protocols describe (1) pilot experiments to determine initial conditions for batch or column ...
Richard J. FitzGerald, David Sheehan
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AbstractThis unit outlines the basic steps in planning and carrying out ion‐exchange chromatography to separate proteins. Protocols describe both batch adsorption and column chromatography in conjunction with either step‐ or linear elution gradients. Support protocols describe (1) pilot experiments to determine initial conditions for batch or column ...
Richard J. FitzGerald, David Sheehan
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Ion-exchange chromatography [PDF]
Analytical Chemistry, 1964 Ion-exchange chromatography is a versatile method of separation and concentration which is applicable to many kinds of analysis, inorganic, organic, and biochemical. The experimental technique is simple and in most cases fairly rapid. In the great majority of inorganic applications, all that is needed is a tube about 25 cm long and 1 cm i.d ...
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Electrically polarized ion-exchange beds in ion chromatography: ion reflux.
Analytical Chemistry, 1998All forms of liquid chromatography use eluents that must be prepared and replenished; a widely applied form of ion chromatography (IC) also requires a suppressor and a means for regenerating it.
H. Small, J. Riviello
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, 2002
Introduction Background Factors Affecting the Ion Exchange of Proteins Stationary-Phase Materials Particle Size Ion Exchange Functional Groups Capacity and Porosity Novel and Traditional ...
S. Cramer, V. Natarajan
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Introduction Background Factors Affecting the Ion Exchange of Proteins Stationary-Phase Materials Particle Size Ion Exchange Functional Groups Capacity and Porosity Novel and Traditional ...
S. Cramer, V. Natarajan
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Analytical Chemistry, 1962
Chromatographic methods using either the synthetic ion-exchange resins or other ion-exchange materials are widely used for the separation of many classes of ionized or ionizable substances. Substances of both high and low molecular weight have been successfully chromatographed on ion-exchange materials.
Robert Kunin, Francis X. McGarvey
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Chromatographic methods using either the synthetic ion-exchange resins or other ion-exchange materials are widely used for the separation of many classes of ionized or ionizable substances. Substances of both high and low molecular weight have been successfully chromatographed on ion-exchange materials.
Robert Kunin, Francis X. McGarvey
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Analytical Chemistry, 2017
Combining process analytical technology (PAT) with continuous production provides a powerful tool to observe and control monoclonal antibody (mAb) fermentation and purification processes.
Bhumit A. Patel +12 more
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Combining process analytical technology (PAT) with continuous production provides a powerful tool to observe and control monoclonal antibody (mAb) fermentation and purification processes.
Bhumit A. Patel +12 more
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Journal of Separation Science, 2016
The mobile phase pH is a key parameter of every ion exchange chromatography process. However, mechanistic insights into the pH influence on the ion exchange chromatography equilibrium are rare.
Simon Kluters +3 more
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The mobile phase pH is a key parameter of every ion exchange chromatography process. However, mechanistic insights into the pH influence on the ion exchange chromatography equilibrium are rare.
Simon Kluters +3 more
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[3] Ion-exchange chromatography
1996Publisher Summary Over the years, electrophoretic techniques were the leading bioanalytical tools for biopolymers. Slab gel electrophoresis has yielded spectacular results, in resolving closely related nucleic acids of high molecular mass. Isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) have served well ...
Gargi Choudhary, Csaba Horváth
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