Lentiviral Vector Purification Using Nanofiber Ion-Exchange Chromatography [PDF]
Molecular Therapy: Methods & Clinical Development, 2019 Lentiviral vectors (LVs) are used in cell and gene therapies due to their ability to transduce both dividing and non-dividing cells while carrying a relatively large genetic payload and providing long-term gene expression via gene integration.
Jelena Ruscic +4 more
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Ion-Exchange Chromatography Coupled to Mass Spectrometry in Life Science, Environmental, and Medical Research. [PDF]
Anal Chem, 2023 anion-exchange chromatography. The column’s stationary phase is positively charged and equilibrated with a mobile phase containing negative ions (e.g., OH − or another anion) at a minimal concentration. When a sample is introduced with negatively charged
Ngere JB +5 more
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Total plasma homocysteine measurement: Evaluation of the Abbott immunoassay, comparison with the JEOL ion exchange chromatography and investigation of its clinical utility [PDF]
Practical Laboratory Medicine, 2022 Objectives: Homocysteine is an intermediary amino acid formed in methionine metabolism, with elevated total homocysteine (tHCY) being a biomarker of cardiovascular and cerebrovascular diseases. We evaluated the Abbott ARCHITECT tHCY immunoassay, compared
Kai Fung Kevin Suen +6 more
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Ion-exchange chromatography purification of extracellular vesicles
BioTechniques, 2017 Despite numerous studies, isolating pure preparations of extracellular vesicles (EVs) has proven challenging. Here, we compared ion-exchange chromatography (IEC) to the widely used sucrose density gradient (SDG) centrifugation method for the purification
Maja Kosanović +4 more
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Water, electricity and ion exchange; how Hamish Small sustained the evolution of ion chromatography
Heliyon, 2021 Since the development of synthetic ion exchange resins in 1935, the electrochemical properties of these unique materials have intrigued researchers. In 1951, Hamish Small was introduced to synthetic ion exchange resin when he studied the electromigration
John M. Riviello
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Online ion-exchange chromatography for small-angle X-ray scattering. [PDF]
Acta Crystallogr D Struct Biol, 2016 SAXS coupled with online ion-exchange chromatography allows the collection of high-quality BioSAXS data.
Hutin S, Brennich M, Maillot B, Round A.
europepmc +2 more sources
Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline. [PDF]
J Vis Exp, 2017 Biological small angle X-ray scattering (BioSAXS) is a powerful technique in molecular and structural biology used to determine solution structure, particle size and shape, and surface-to-volume ratio of macromolecules.
Brennich ME, Round AR, Hutin S.
europepmc +2 more sources
Consecutive Affinity and Ion-Exchange Chromatography for AAV9 Vectors Purification [PDF]
BiomedicinesBackground: Irrespective of the rapid development of AAV-based gene therapy, the production of clinical-grade vectors has a bottleneck resulting from product-related impurities such as empty and partially filled capsids, which lack a functional ...
Ozgun Firat Duzenli, George Aslanidi
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Ion exchange membranes in ion chromatography and related applications
Talanta, 2019 This review is a broad survey of ion exchange membranes (IEMs) in chosen areas of analytical chemistry. The coverage is comprehensive in ion chromatography (IC) applications, this includes in-line water purification for IC use, chemical and ...
Purnendu Dasgupta
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Hydrophobicity of polymer based anion-exchange columns for ion chromatography
Heliyon, 2021 The regularities of the retention of alkanoic and alkanesulfonic acids homologues were investigated for the set of 36 anion-exchange columns produced by various manufacturers.
Pavel N. Nesterenko +1 more
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