Results 41 to 50 of about 284,335 (284)

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

Isotope effects of neodymium in different ligands exchange systems studied by ion exchange displacement chromatography

Journal of Advanced Research, 2013
The isotope effects of neodymium in Nd-glycolate ligand exchange system were studied by using ion exchange chromatography. The separation coefficients of neodymium isotopes, ε’s, were calculated from the observed isotopic ratios at the front and rear ...
Ibrahim Ismail, Ahmed S. Fawzy, Mohammad I. Ahmad, Hisham F. Aly, Masao Nomura, Yasuhiko Fujii   +5 more
doaj   +1 more source

Peptidomic and glycomic profiling of commercial dairy products: identification, quantification and potential bioactivities. [PDF]

, 2019
Peptidomics and glycomics are recently established disciplines enabling researchers to characterize functional characteristics of foods at a molecular level.
Barile, Daniela, Bhattacharya, Mrittika, Henrick, Bethany Michele, Robinson, Randall C, Salcedo, Jaime   +4 more
core   +2 more sources

Novel ionization reagent for the measurement of gas-phase ammonia and amines using a stand-alone atmospheric pressure gas chromatography (APGC) source. [PDF]

, 2020
RationaleContaminants present in ambient air or in sampling lines can interfere with the target analysis through overlapping peaks or causing a high background. This study presents a positive outcome from the unexpected presence of N-methyl-2-pyrrolidone,
Finlayson-Pitts, Barbara J, Li, Xiaoxiao, Perraud, Véronique, Smith, James N   +3 more
core   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Discovery and Targeted Proteomic Studies Reveal Striatal Markers Validated for Huntington's Disease

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Clinical trials for Huntington's disease (HD) enrolling persons before clinical motor diagnosis (CMD) lack validated biomarkers. This study aimed to conduct an unbiased discovery analysis and a targeted examination of proteomic biomarkers scrutinized by clinical validation. Methods Cerebrospinal fluid was obtained from PREDICT‐HD and
Daniel Chelsky, Cara Joyce, H. Jeremy Bockholt, Paul A. Rudnick, William H. Adams, Fiona McAllister, Justin W. Smock, Michael A. Newton, Jane S. Paulsen   +8 more
wiley   +1 more source

Simultaneous determination of several forms of phosphate in food by ion exchange chromatography

Tạp chí Kiểm nghiệm và An toàn thực phẩm
The phosphate group is a group of food additives commonly used for moisturizing, improving the structure, and retaining the color and flavor of products prepared from meat, fish, milk, baked goods, and beverages.
Do Thi Hong Thuy, Nguyen Nhu Thuong, Vu Thi Trang, Le Thi Hong Hao, Le Thi Thuy, Le Viet Ngan, Luu Thi Huyen Trang, Cao Cong Khanh, Dinh Viet Chien, Le Thi Phuong Thao, Nguyen Thi Anh Huong   +10 more
doaj   +1 more source

Formation of dimers in refolding of recombinant human interferon-γ by ion-exchange chromatography

浙江大学学报. 农业与生命科学版, 2006
The refolding process was performed by gradually decreasing the concentration of urea in the buffer after the denatured rhIFN-γ proteins had been bound onto the ion-exchange gel SP Sepharose Fast Flow.
JIN Ting, GUAN Yi-xin, YAO Shan-jing
doaj   +1 more source

Concanavalin A-Nonbinding Enzymes of Crotalus scutulatus scutulatus Venom [PDF]

, 1986
Crotalus scutulatus scutulatus crude venom was separated into two fractions by Concanavalin A Sepharose 4B affinity chromatography. The Concanavalin A-nonbinding fraction (F-l) exhibited phosphomonoesterase (orthophosphoric monoester phosphohydrolase EC ...
Childs, C. K., Hinson, Michael W., Johnson, Bob D., Sifford, Dewey H.   +3 more
core   +1 more source

Zein‐Based Adhesives: Sustainable Extraction and Application in Bioadhesive Technologies

Advanced Engineering Materials, EarlyView.
Zein is extracted from corn gluten meal using a simple and scalable process with high yield (~90%). The resulting protein is applied in bioadhesives modified with Ca2+ and Fe3+ ions, exhibiting substrate‐dependent adhesion. The findings demonstrate competitive bonding performance and highlight the role of ionic interactions in tuning adhesion ...
Paula Bertolino Sanvezzo, Daniela Winsley Valereto Friozi, Vinícius de Oliveira, Marcia Cristina Branciforti   +3 more
wiley   +1 more source