Results 61 to 70 of about 3,527,182 (276)

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

A minimal cellulosome‐like system in Cellulosilyticum lentocellum

FEBS Open Bio, EarlyView.
Cellulose‐degrading bacteria typically use cellulosomes, large multi‐enzyme complexes on a scaffold protein. In Cellulosilyticum lentocellum, we characterise a far smaller arrangement, a single scaffold bound to one cellulase through a single cohesin‐dockerin interaction.
John Allan, Amias Alstrom‐Moore, Gary W Black   +2 more
wiley   +1 more source

ION EXCHANGE CHROMATOGRAPHY WITH CONDUCTIVITY DETECTION COMPARED TO SPECTROPHOTOMETRY TO ASSAY PHOSPHATE IN MILK AND MILK PRODUCTS

Jurnal Teknologi dan Industri Pangan, 2015
Phosphorus is a critical dietary mineral that is involved as phosphate in bone, tooth, nucleic acids or phospholipids and is mainly supplied through consumption of dairy products.
Tia Amina Setiawati, Fanny Guyomarc’h, Eric Beaucher, Frederic Gaucheron   +3 more
doaj   +1 more source

Production and Purification of a Polyclonal Antibody Against Purified Mouse IgG2b in Rabbits Towards Designing Mouse Monoclonal Isotyping Kits [PDF]

Advanced Pharmaceutical Bulletin, 2015
Purpose: Mouse IgG subclasses containing IgG1, IgG2a, IgG2b and IgG3 have been defined and described both physiochemically and immunologically. Methods: Sepharose beads conjugated with protein A affinity chromatography was used for purification of mouse
Sadeq Eivazi, Jafar Majidi, Leili Aghebati Maleki, Jalal Abdolalizadeh, Mehdi Yousefi, Majid Ahmadi, Somayeh Dadashi, Zahra Moradi, Elmira Zolali   +8 more
doaj   +1 more source

Identification and characterization of a gene conferring stress resistance in Escherichia coli and cyanobacteria

FEBS Open Bio, EarlyView.
Functional screening identified PcSyn14890, a cyanobacteria‐specific protein that enhances growth and stress resistance in E. coli and Synechocystis PCC6803. Although we expected it to function as a molecular chaperone, it was unable to protect against thermal aggregation of GAPDH.
Akiyo Yamada, Takaaki Tanaka, Manaka Uehara, Keigo Nakano, Ken Morishima, Rintaro Inoue, Masaaki Sugiyama, Masafumi Yohda   +7 more
wiley   +1 more source

Synthesis of Tripotassium Trimetaphosphate from Trisodium Trimetaphosphate via Solution/Precipitation Ion Exchange Cycles

Compounds
Cyclic trimetaphosphate (cTmp) has been used as a prebiotically plausible compound to generate chemically activated nucleoside 5′-phosphates. While cTmp is mainly available as a trisodium salt, some experiments benefit from different cations.
Ulrich F. Müller
doaj   +1 more source

Morphology, Transport, and Dynamics of Protein Adsorption in Open‐Cell Metal Foam

Advanced Engineering Materials, EarlyView.
Stainless steel (SS) open‐cell foams are shown to adsorb more protein per unit area than previously reported 316L SS and chromium oxide surfaces under static and flow conditions. An integrated approach combining 3D pore imaging, flow simulation, and protein adsorption experiments characterizes the foam’s performance.
Chinmaya Prerana Inguva, Joseph Lavoie, Afsaneh Rabiei   +2 more
wiley   +1 more source

Isomorphous Substitution of P(V) in Natural Clinoptilolite: Evalution of the Product for Removal of NO3-, NO2- and F - from Aqueous Solutions [PDF]

Iranian Journal of Chemistry & Chemical Engineering, 2008
Nitrate is an anion of major importance particularly in biological areas. Excessive amounts in water supplies indicate pollution from sewage or agricultural effluents. The effects of excessive quantities of nitrate in water are well known.
Hossein Faghihian, Mohammad Hadi Mohammadi   +1 more
doaj  

Conformational Switching of Proteins on Material Surfaces Enabled by Peptide‐Oriented Adsorption

Advanced Functional Materials, EarlyView.
Responsive biointerfaces could be achieved using conformationally responsive proteins; however, their interconversion can be lost upon surface adsorption. Herein, we demonstrate that the incorporation a Au binding peptide into calmodulin allows for binding control on Au wherein the protein retains conformational switching. This capability was confirmed
Sakthirupini Ramamurthy, Kyle B. Meerbott, Lorenzo Travaglini, Garima S. Dobhal, Artur Sawicki, Marc R. Knecht, Tiffany R. Walsh, Dominic J. Glover   +7 more
wiley   +1 more source

Recent advances in polymer monoliths for ion-exchange chromatography

, 2009
The use of polymeric materials in ion-exchange chromatography applications is advantageous because of their typically high mechanical stability and tolerance of a wide range of pH conditions.
Hilder, E., Nordborg, A.
core   +2 more sources