Results 231 to 240 of about 370,485 (298)
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Analytica Chimica Acta, 1967
Abstract A review is given of variables which affect the separation factors in ion-exchange chromatography. In favorable cases the influence of complex formation, pH, and eluant concentration can be predicted by simple calculations, whereas the interaction forces inside the resin phase are still largely unexplored.
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Abstract A review is given of variables which affect the separation factors in ion-exchange chromatography. In favorable cases the influence of complex formation, pH, and eluant concentration can be predicted by simple calculations, whereas the interaction forces inside the resin phase are still largely unexplored.
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Chapter 22 Ion-Exchange Chromatography
2009Ion-exchange chromatography is the most popular chromatographic method for separation of proteins. It is a versatile and generic tool and is suited for discovery of proteins, high-resolution purification, and industrial production of proteins. Separation conditions are within physiological range of salt and pH and in the most cases a native protein can
Alois Jungbauer, Rainer Hahn
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Modeling of salt and pH gradient elution in ion-exchange chromatography.
Journal of Separation Science, 2014The separation of proteins by internally and externally generated pH gradients in chromatofocusing on ion-exchange columns is a well-established analytical method with a large number of applications.
Michael Schmidt +2 more
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Ion chromatography on methacrylate ion exchangers
Journal of Chromatography A, 1989Abstract The preparation and properties of ion chromatography sorbents based on glycidyl methacrylate—ethylene dimethacrylate macroporous copolymers are described. Anion exchangers were suitable for the separation of inorganic anions and possessed a high selectivity towards the NO - 3 anion.
L.D. Beljakova +5 more
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THE PURIFICATION OF TRYPSINOGEN BY ION-EXCHANGE CHROMATOGRAPHY
Canadian Journal of Biochemistry and Physiology, 1962The purification of trypsinogen by several methods of ion-exchange chromatography has been investigated. The use of phosphate–urea buffers on Bio-Rex 70 was not a suitable preparative procedure because of considerable inactivation of the zymogen. Chromatography on carboxymethyl cellulose at pH 3.2 produced no significant increase in the potential ...
G. Y. Mabbott +2 more
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[24] Ion-exchange chromatography
1990Publisher Summary This chapter introduces ion-exchange chromatography (IEC) as a method for the purification of proteins and enzymes. When proteins are to be purified by IEC, some problems arise because proteins have unique origins. Proteins are derived from biological sources and their extraction often requires the use of detergents and chaotropic ...
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Ion Exchange in Paper Chromatography
Nature, 1953IT has been shown by Schute that several mixtures of alkaloids can be separated by chromatography on paper using aqueous ammonia1. Because the chromatograms have the characteristics of a partition chromatogram, it is probable that the above separation is due to partition between an aqueous imbibition phase and an aqueous mobile phase.
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Model‐Based Process Challenge of an Industrial Ion‐Exchange Chromatography Step
, 2012The separation of one weakly and one strongly binding impurity from a therapeutic protein by ion-exchange chromatography was studied with a mechanistic model.
Karin Westerberg +4 more
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Ion-Exchange Chromatography in Alcohol
Nature, 1955IN the technique of ion-exchange chromatography, the order in which compounds are eluted from a column depends basically upon differences in their ionization constants; but other factors modify and in many instances reverse the expected order1. This is illustrated in Fig.
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Robust mechanistic modeling of protein ion-exchange chromatography.
Journal of Chromatography A, 2021Vijesh Kumar +6 more
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