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Advantages of ion-exchange chromatography for oligonucleotide analysis.
Bioanalysis, 2011The rapid development of therapeutic oligonucleotides (ONs) has created a need for in-depth characterization of ONs, beyond previous requirements. The natural migration to LC-MS requires the use of chromatography with MS-compatible eluents to introduce ...
K. Cook, J. Thayer
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, 2012
Hydrophobic interaction and ion exchange chromatography are basic steps in purification of fermentative biopharmaceuticals. An optimization by statistical design of experiments requires a huge amount of feed. An alternative approach is the combination of
C. Helling, C. Borrmann, J. Strube
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Hydrophobic interaction and ion exchange chromatography are basic steps in purification of fermentative biopharmaceuticals. An optimization by statistical design of experiments requires a huge amount of feed. An alternative approach is the combination of
C. Helling, C. Borrmann, J. Strube
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Ion Exchange in Ion Chromatography
1989Of the many methods that have been applied to the chromatographic separation of ions, most employ ion exchangers. The purpose of this chapter is to describe some of the more important phenomena associated with the ion exchange process, to explain their physical causes, and to demonstrate how principles and theories of ion exchange relate to the ...
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Ion exchange paper chromatography
Journal of Chemical Education, 1961Describes an experiment/demonstration in ion exchange paper chromatography separating amino acids.
Alan T. Thomas, J. P. Phillips
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1979
Ion exchangers consist of an insoluble framework (matrix) containing covalently bonded dissociable functional groups at accessible sites. These are either sulfonic acid groups or, less importantly, carboxyl groups in the case of cation exchangers, and tertiary amino or quaternary ammonium groups for anion exchangers.
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Ion exchangers consist of an insoluble framework (matrix) containing covalently bonded dissociable functional groups at accessible sites. These are either sulfonic acid groups or, less importantly, carboxyl groups in the case of cation exchangers, and tertiary amino or quaternary ammonium groups for anion exchangers.
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Ion-Exchange Chromatography of Proteins
2003Ion-exchange chromatography is the most widely used technique in protein chromatography (1). This is because it is nearly always possible to develop successful ionexchange separations for proteins, and the materials required are relatively inexpensive.
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Ion-exchange chromatography of nitrogen compounds
Journal of Chromatography A, 1974Abstract The review presents a systematic survey of ion-exchange methods for the analysis of nitrogen-containing compounds. The main interest is in cation-exchange separations of amines, nitrogen-containing bases and related compounds by elution with solutions of acids, buffers and salts and aqueous organic solvents and in highspeed chromatography of
Pavel Jandera, Jaroslav Churáček
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Electromodulated ion exchange chromatography
Analytical Chemistry, 1986On montre que les proprietes de la colonne peuvent etre controlees par l'intermediaire de l'etat electrochimique de la ...
Charles R. Martin, Amiya R. Ghatak-Roy
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Analysis by Chromatography and Ion Exchange
Nature, 1954Inorganic Chromatography By Orsino C. Smith. Pp. x + 134 + 5 plates. (New York: D. Van Nostrand Company, Inc.; London: Macmillan and Co., Ltd., 1953.) 37s. 6d. net. Chromatographic Methods of Inorganic Analysis With Special Reference to Paper Chromatography. By Dr. F. H. Pollard and Dr. J. F. W. McOmie. Pp. viii + 192 + 4 plates.
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Ion-exchange chromatography of tissue nucleotides
Journal of Chromatography A, 1969Abstract A procedure has been devised for the isolation of soluble tissue nucleotides by ion-exchange chromatography on 1.0 × 90 cm columns of DEAE-Sephadex A-25, acetate form, using consecutive concave concentration gradients of the volatile salt triethylammonium acetate at pH 4.7.
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