Results 101 to 110 of about 1,326,002 (312)

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum

FEBS Open Bio, EarlyView.
Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik, Russell Pickford, Hannah C. Timmins, Olivier Piguet, Glenda M. Halliday, Matthew C. Kiernan, Woojin Scott Kim   +6 more
wiley   +1 more source

Separation of whey proteins for chromatography liquid

Scientia Agropecuaria, 2010
This paper describes and compares three chromatographic methods for the analysis and quantification of most abundant proteins in cheese whey, -lactalbumin and -lactoglobulin. The methods were: Reverse-phase high performance liquid chromatography, anion
Abraham D. Giraldo Zuñiga, Edwin E.García Rojas, Jane S. R.Coimbra, Wilmer E. Luera Peña   +3 more
doaj   +1 more source

Use of high pressure liquid chromatography in the study of liquid lubricant oxidation [PDF]

The general principles of classical liquid chromatography and high-pressure liquid chromatography (HPLC) are reviewed, and their advantages and disadvantages are compared.
Morales, W.
core   +1 more source

Competitive adsorption of plasma proteins at solid—liquid interfaces [PDF]

, 1986
The competitive adsorption of human serum albumin (HSA), human immuno-γ-globulin (HIgG) and human fibrinogen (HFb) onto polystyrene (PS) at 20° C and a pH of 7.35 (phosphate-buffered saline) was studied.
Breemhaar, W., Feijen, J., Lensen, H.G.W., Smolders, C.A.   +3 more
core   +3 more sources

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Tryptic peptide analysis of protein binders in works of art by liquid chromatography–tandem mass spectrometry [PDF]

, 2008
Comprehension of the materials, such as binding media, used by artists is of uttermost importance in restoration and in art historical studies. The most frequently used binders are drying oils and proteins; in this study focus is placed on proteins ...
Deforce, Dieter, Dhaenens, Maarten, Fremout, Wim, Moens, Luc, Sanyova, Jana, Saverwyns, Steven, Vandenabeele, Peter   +6 more
core  

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

RECENT ADVANCES IN GAS, LIQUID AND SUPERCRITICAL FLUID CHROMATOGRAPHY IN THE CONTEXT OF SUSTAINABLE/ GREEN CHEMISTRY

International Journal of Pharmacy & Integrated Health Sciences
Background: Chromatographic techniques such as gas chromatography (GC), liquid chromatography (LC) and supercritical fluid chromatography (SFC) have long been employed in analytical chemistry. However, using or producing certain organic solvents and non-
Nimra Sohail, Bushra Naureen, Mehmood Asghar, Aziza Sarwar, Aisha Altaf   +4 more
doaj   +1 more source

Analysis of Cordyceps by multi-column liquid chromatography

Acta Pharmaceutica Sinica B, 2017
Cordyceps is a famous traditional Chinese medicine (TCM) that has been used in China for hundreds of years. In the present study a multi-column liquid chromatography (MC-LC) system was developed for the qualitative analysis of macromolecules and ...
Zhengming Qian, Shaoping Li
doaj   +1 more source