Results 81 to 90 of about 846,106 (312)
Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10
FEBS Open Bio, EarlyView.Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee +6 more
wiley +1 more source
Ionic liquids in liquid chromatography
Journal of Chromatography OpenThe use of ionic liquids (ILs) in liquid chromatography (LC) is reviewed. ILs have several different types of functionalities, which means that they are prone to multiple simultaneous interactions with stationary phases in LC.
Sini Santasalo, Susanne K. Wiedmer
doaj +1 more source
A yeast model of 5‐oxoproline accumulation reveals a general toleration to 5‐oxoproline
FEBS Open Bio, EarlyView.Using a yeast model, we show that even high accumulation of 5‐oxoproline causes only mild cellular stress and does not trigger oxidative stress. Instead, cells adapt by activating efflux pumps and diverse protective pathways, suggesting that previously proposed harmful effects of 5‐oxoproline may arise from indirect metabolic imbalances rather than the
Pratiksha Dubey +4 more
wiley +1 more source
Application of liquid chromatography to the determination of emerging pollutants in environmental samples [PDF]
, 2013 Emerging contaminants are compounds that are not currently covered by existing regulations, that have not been previously studied, and that are thought to be a possible threat to environmental health and safety.
Mahugo Santana,Cristina Isabel +2 more
core
Determination of β-Galactooligosaccharides by Liquid Chromatography
International Journal of Analytical Chemistry, 2014 Beta-galactooligosaccharides (GOS) are oligosaccharides normally produced industrially by transgalactosylation of lactose. They are also present naturally in the milk of many animals including humans and cows. GOS are thought to be good for health, being
Sean Austin +4 more
doaj +1 more source
Journal of Open Research Software, 2016 This paper aimed to develop a standalone application for optimizing flow rates in liquid chromatography (LC), gas chromatography (GC) and supercritical fluid chromatography (SFC). To do so, Van Deemter’s equation, Knox’ equation and Golay’s equation were
Joris Meurs
doaj +1 more source
[Off-line comprehensive two-dimensional countercurrent chromatography-liquid chromatography separation of Curcuma volatile oil]. [PDF]
Se Pu, 2023 Tong X +5 more
europepmc +1 more source
FEBS Open Bio, EarlyView.Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata +13 more
wiley +1 more source
High-performance liquid chromatographic separation of metal carbonyl complexes substituted with bridging and chelating 1,1'-bis(diphenylphosphino)ferrocene [PDF]
, 1991 The high-performance liquid chromatography of metal carbonyl complexes including those containing the 1,1'-bis(diphenylphosphino)ferrocene ligand is reported. Separations were performed on a column packed with silica bonded with polar amino-cyano groups.
Phang, L. T. +3 more
core +1 more source
Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source