Results 81 to 90 of about 2,374,391 (306)

Modeling of Preparative Liquid Chromatography

, 2013
Preparative chromatography is today the best generic method for the purification of small drugs and valuable chemical components at the <10 kg-level. Recent progress in computer technology and the development of new nonchiral and chiral stationary
J. Samuelsson, P. Forssén, T. Fornstedt, Forssén, Patrik,, Samuelsson, Jörgen,, Fornstedt, Torgny,   +5 more
core   +1 more source

Optimization of seminal extracellular vesicle isolation protocols for cancer biomarker analysis using proteomic profiling

Molecular Oncology, EarlyView.
This paper shows that size‐exclusion chromatography and sequential centrifugation applied to seminal plasma enrich for different populations of extracellular vesicles. This may significantly affect the detection and analysis of extracellular vesicle‐associated cancer biomarkers.
Elen Telumyan, Beatriz Martín‐Gracia, Eva Bassols‐Citores, Krizia Sagini, Maria K. Andersen, Tuula A. Nyman, May‐Britt Tessem, Morten B. Rye, Alicia Llorente   +8 more
wiley   +1 more source

Plasma L-ergothioneine measurement by high-performance liquid chromatography and capillary electrophoresis after a pre-column derivatization with 5-iodoacetamidofluorescein (5-IAF) and fluorescence detection [PDF]

, 2013
Two sensitive and reproducible capillary electrophoresis and high-performance liquid chromatography-fluorescence procedures were established for quantitative determination of L-egothioneine in plasma.
Pintus, Gianfranco, Angelo Zinellu, Erre, Gian Luca, Carru, Ciriaco, Salvatore Sotgia, Ciriaco Carru, Sotgia, Salvatore, Erre GL, Gianfranco Pintus, Zinellu, Angelo, Deiana, Luca, Gerard Aime Pinna, Luca Deiana, Pisanu, Elisabetta, Elisabetta Pisanu, Gian Luca Erre, Pinna, Gérard Aimé, Sotgia S, Pisanu Elisabetta, Zinellu Angelo, Deiana Luca, Sotgia Salvatore, Pinna Gerard Aime, Erre Gian Luca, Pintus Gianfranco, Carru Ciriaco   +25 more
core   +1 more source

Tumour‐proximal deep serum proteomics identifies biomarkers associated with ovarian cancer

Molecular Oncology, EarlyView.
New biomarkers are needed for early ovarian cancer detection. Ovarian (tumour‐proximal) and peripheral vein serum from healthy women and patients with benign or malignant tumours underwent nanoparticle‐enriched deep proteomics. Ovarian serum yielded more protein identifications and revealed tumour‐reflective signatures.
Darren Lau, Daniel Pincher, Clifford Young, Nicholas Chappell, Noor A. Lokman, Parul Mittal, Leigh Donnellan, Martin K. Oehler, Doug A. Brooks, Peter Hoffmann   +9 more
wiley   +1 more source

Ion exchange chromatography – basic principles and application [PDF]

, 2011
Ion-Exchange Chromatography (IEC) allows for the separation of ionizable molecules on the basis of differences in charge properties. Its large sample-handling capacity, broad applicability (particularly to proteins and enzymes), moderate cost, powerful ...
Dowling, Oonagh, Cummins, Phil, O'Connor, Brendan   +2 more
core   +2 more sources

Application of Liquid Chromatography in Food Analysis

Foods, 2020
Food products are very complex mixtures consisting of naturally-occurring compounds and other substances, generally originating from technological processes, agrochemical treatments, or packaging materials [...]
Oscar Núñez, Paolo Lucci
doaj   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Automated processing of whole blood samples for the determination of immunosuppressants by liquid chromatography tandem-mass spectrometry [PDF]

, 2006
Background: Liquid chromatography tandem-mass spectrometry (LC-MS/MS) is an efficient technology for routine determination of immunosuppressants in whole blood; however, time-consuming manual sample preparation remains a significant limitation of this ...
Spöhrer, Ute, Vogeser, Michael
core   +1 more source

Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface

FEBS Open Bio, EarlyView.
HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana, Avigail Yariv, Tal Oppenheim, Hadar Amartely, Dana Reichmann, Oded Livnah   +5 more
wiley   +1 more source

Ionic liquids in liquid chromatography

Journal of Chromatography Open
The use of ionic liquids (ILs) in liquid chromatography (LC) is reviewed. ILs have several different types of functionalities, which means that they are prone to multiple simultaneous interactions with stationary phases in LC.
Sini Santasalo, Susanne K. Wiedmer
doaj   +1 more source